Nocardioides mangrovi sp. nov., a novel endophytic actinobacterium isolated from root of Kandelia candel

Abstract

A novel species of endophytic actinobacterium, designated strain GBK3QG-3T, was isolated from surface-sterilized root of Kandelia candel collected from Beilun Estuary, Guangxi Zhuang Autonomous Region, PR China. Cells of strain GBK3QG-3T were Gram-stain-positive, aerobic, non-spore-forming and rod-shaped. Growth of GBK3QG-3T was observed at 10–40 °C (optimum, 30 °C), pH 5.0–9.0 (optimum, pH 7.0) and in the presence of 0–3 % (w/v) NaCl (optimum, 0 %). The G+C content of genomic DNA was determined to be 72.2 mol%. On the basis of 16S rRNA gene sequence analysis, strain GBK3QG-3T was most related to the type strain of Nocardioides agrisoli CCTCC AB 2017058T (97.7 % 16S rRNA gene sequence similarity), followed by Nocardioides ginkgobilobae SYP-A7303T (97.0 %) and Nocardioides aquiterrae GW-9T (96.9 %). Average nucleotide identity and digital DNA–DNA hybridization values between strain GBK3QG-3T and some strains of the genus Nocardioides with publicly available genomes were below 84.3 and 30.0 %, respectively. The cell-wall peptidoglycan contained ll-diaminopimelic acid. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, an unknown phospholipid and an unknown lipid. The major menaquinone was identified as MK-8(H4), and the major fatty acids were identified as iso-C16 : 0, iso-C17 : 0 and anteiso-C17 : 0. Based on evidence collected from the phenotypic, genotypic and phylogenetic analyses, we propose a novel species named Nocardioides mangrovi sp. nov. The type strain is GBK3QG-3T (=CGMCC 4.7708T=JCM 34553T).

Cultivable Endophyte Resources in Medicinal Plants and Effects on Hosts

“…Actinomycetes of other genera can also be isolated from medicinal plants, but the number is relatively small compared with Streptomyces. Many studies have isolated new species of bioactive endophytic actinomycetes from medicinal plants, which greatly expanded microbial resources and laid a foundation for the industrial application of actinomycetes [ 52 , 53 , 58 , 60 , 61 ]. The results of endophyte isolation from medicinal plants in recent years are shown in Table 3 .…”

Nocardioides agri sp. nov., isolated from garden soil

“…Bacteria have numerous beneficial uses for human welfare [9, 10]. Members of the genus Nocardioides have various applications including the production of valuable enzymes, antibiotics, biodegradation of hazardous chemicals, and plant growth-promoting activities, etc [2–4, 8, 11]. In the present study, we describe a novel bacterial species of genus Nocardioides , Nocardioides agri sp.…”

“…At the time of writing, the genus Nocardioides includes 166 species with validly published names (https://lpsn.dsmz.de/genus/nocardioides). The common characteristics of the members of genus Nocardioides include Gram-stain-positive, aerobic, rod or cocci-shaped, and motile or non-motile cells with high DNA G+C content, in a range of 68.7-74.9 mol% [2][3][4][5][6][7][8]. Members of this genus contain menaquinone MK-8 (H 4 ) as the predominant isoprenoid quinone.…”

“…Members of this genus contain menaquinone MK-8 (H 4 ) as the predominant isoprenoid quinone. The major fatty acid of the members of this genus was determined to be C 16:0 iso [2][3][4][5][6][7][8]. Bacteria have numerous beneficial uses for human welfare [9,10].…”

Paenibacillus caseinilyticus sp. nov., isolated forest soil

“…Paenibacillus are abundantly distributed in environments such as soil, sediment, water, hot springs, freshwater wetlands, rhizosphere, root nodules, phyllosphere, food, insects and a necrotic wound [5][6][7][8][9][10]. Recently, two species, Paenibacillus rhizolycopersici and Paenibacillus mangrovi, were isolated from a plant habitat and a marine environment, respectively [11,12]. This study characterizes and details the taxonomic status of strain GW78 T , which was obtained from a forest soil environment during a study of cultivable microbial communities.…”