Nocardioides panacis sp. nov., isolated from soil of a ginseng field

Abstract

A bacterial strain designated as G188T was isolated from ginseng field soil in the Republic of Korea. Phylogenetic analysis of 16S rRNA gene sequences showed that strain G188T formed a distinct lineage within the genus Nocardioides, family Nocardioidaceae , order Propionibacteriales . Sequence similarity revealed that strain G188T was most closely related to Nocardioides iriomotensis IR27-S3T (97.7 % 16S rRNA similarity). The genome size of strain G188T was 4 901 775 bp, and the genomic DNA G+C content was 72.3 mol%. The average nucleotide identity and DNA–DNA hybridization values with other Nocardioides species were less than 75.6 and 20.1 %, respectively. The main fatty acids of strain G188T were C17 : 0, C17 : 1 ω8c and iso-C16 : 0. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylinositol, and the major respiratory quinone was menaquinone 8, supporting that strain G188T was affiliated with the genus Nocardioides . Based on biochemical, chemotaxonomic and phylogenetic analyses, the novel species Nocardioides panacis G188T (KACC 21695T=LMG 31733T) is proposed.

Nocardioides bizhenqiangii sp. nov. and Nocardioides renjunii sp. nov., isolated from soil and faeces of Tibetan antelope (Pantholops hodgsonii) on the Qinghai-Tibet Plateau

“…The genus Nocardioides, a member of the family Nocardioidaceae within the order Propionibacteriales in the class Actinomycetes, was first described by Prauser in 1976 with Nocardioides albus as the type species [1,2]. At the time of writing, 166 species have been validly named and published in LPSN (https://lpsn.dsmz.de/genus/Nocardioides, accessed May 2024) [3], which were isolated from diverse sources, such as faeces of wild animals [4,5], soil [6,7], plants [8,9], and other environments [10][11][12][13][14]. Members of the genus Nocardioides are aerobic, Gram-stain-positive, mesophilic, pH-neutral, coccus-or rod-shaped bacteria.…”