Nocardiopsis ansamitocini sp. nov., a new producer of ansamitocin P-3 of the genus Nocardiopsis

Abstract

Nocardiopsis ansamitocini sp. nov., a new producer of ansamitocin P-3 of the genus Nocardiopsis The genus Nocardiopsis was first proposed by Meyer (1976), and later affiliated with the family Nocardiopsaceae (Rainey et al., 1996). At time of writing, the genus comprised 38 recognized species and two subspecies (http://www. bacterio.net/nocardiopsis.html), although Nocardiopsis arabia was recently reclassified as Streptomonospora arabica (Zhang et al., 2013). Novel species of the genus Nocardiopsis are increasingly being reported, such as Nocardiopsis algeriensis (Bouras et al., 2015), Nocardiopsis mangrovei (Huang et al., 2015), and Nocardiopsis oceani and Nocardiopsis nanhaiensis (Pan et al., 2015). Nocardiopsis species harbour characteristic features: the substrate mycelium may fragment into rods or cocci, production of aerial hyphae varies from sparse to abundant, and the cell wall contains 2,6-mesodiaminopimelic acid (meso-DAP) but no diagnostic sugar (Meyer, 1976). Most members of the genus Nocardiopsis are from saline or alkaline environments, and may adopt complicated hyphal differentiations to adapt to these extreme environments, by which they acquire the ability to form some bioactive products (Horikoshi, 1999;Ding et al., 2010Ding et al., , 2012 Bérdy, 2012). During research into Actinobacteria with application in biotechnology, a 3-amino-5-hydroxybenzoate (AHBA) synthase gene PCR screen-positive strain, designated EGI 80425 T , was found to form ansamitocin P-3, an anti-tumour metabolite, which has been reported to be produced by Actinosynnema pretiosum subsp. pretiosum No. C-15003 (Higashide et al., 1977), 'Claopodium crispifolium', 'Anomodon attenuatus' and related actinomycetes (Suwanborirux et al., 1990). In this study, strain EGI Abbreviations: meso-DAP, 2,6-meso-diaminopimelic acid.

Kineococcus vitellinus sp. nov., Kineococcus indalonis sp. nov. and Kineococcus siccus sp. nov., Isolated Nearby the Tabernas Desert (Almería, Spain)

“…The genomic G + C content of strains T13 T , T90 T and R8 T was 75.4%, 76.3% and 75.1%, respectively. This genomic G + C content is in accordance with other Kineococcus species, and further confirms their adscription to the Kineococcus genus [ 2 , 7 , 8 ]. A total of 5039, 4690 and 4633 coding sequences (CDSs) were predicted for strains T13 T , T90 T and R8 T , respectively, of which 1894, 2906 and 2832 were proteins with functional assignments.…”

“…Furthermore, the synthesis of meso-diaminopimelic acid in the three strains was predicted based on the presence of the enzyme diaminopimelate epimerase (EC 5.1.1.7), and the synthesis of menaquinones was predicted based on the identification of the enzyme demethylmenaquinone methyltransferase (EC 2.1.1.163). Therefore, the chemotaxonomic profile of the three new strains is in accordance with other Kineococcus species, corroborating their inclusion into the genus Kineococcus [ 2 , 7 , 8 ].…”

“…The genus Kineococcus, belonging to the family Kineosporiaceae [ 1 ], was first proposed by Yokota et al in 1993 [ 2 ]. At the time of writing, the genus Kineococcus is comprised of 11 species with valid published names ( ) which were isolated from soil [ 2 , 3 , 4 , 5 ], saline environments [ 6 , 7 , 8 ], plants [ 9 , 10 ] and radioactive work areas [ 11 ]. This genus includes species with the ability to grow in a wide range of temperatures [ 5 ] and high salt concentrations [ 8 , 9 ].…”

Subtilisin-Involved Morphology Engineering for Improved Antibiotic Production in Actinomycetes

“…The antitumor agent ansamitocins, structurally similar to maytansines from Maytenus serrata [ 23 ], are produced by several genera of actinomycetes, including Actinosynnema [ 24 , 25 ], Amycolatopsis [ 26 , 27 ] and Nocardiopsis [ 28 ]. For large scale fermentation, ansamitocins are produced with A. pretiosum subsp.…”

Transcriptomic and Ectoine Analysis of Halotolerant Nocardiopsis gilva YIM 90087T Under Salt Stress

“…Members of this genus are widely distributed in hypersaline environments and produce very distinct secondary metabolites ( Tsujibo et al, 2003 ; Sun et al, 2015 , 2017 ; Sharma and Singh, 2016 ). In past few years, attempts to isolate novel Nocardiopsis strains were made and many novel species such as N. algeriensis ( Bouras et al, 2015 ), N. mangrovei ( Huang et al, 2015 ), N. oceani and N. nanhaiensis ( Pan et al, 2015 ), N. ansamitocini ( Zhang et al, 2016b ), N. sediminis ( Muangham et al, 2016 ), N. rhizosphaerae ( Zhang et al, 2016c ), and N. akesuensis ( Gao et al, 2016 ) have been reported. At present, the genus comprises 53 species and 5 subspecies ( 1 2017).…”