Nocardiopsis coralliicola sp. nov., isolated from the gorgonian coral, Menella praelonga

Li, Jie; Yang, Jian; Zhu, Wen‐Yong; He, Jie; Tian, Xinpeng; Xie, Qiang; Zhang, Si; Li, Wenjun

doi:10.1099/ijs.0.035402-0

Cited by 25 publications

(13 citation statements)

References 49 publications

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“…Among the 42 hitherto reported species, 13 are halophilic and six are halotolerant. They have been isolated from marine sediments (Fang et al 2011;Meena et al 2013), hypersaline solar salterns (Chun et al 2000;Jose and Jebakumar 2012), saline soils (Al-Tai and Ruan 1994;Li et al 2003;Li et al 2006;Chen et al 2010) and marine biological forms (Chen et al 2009;Li et al 2012). In addition, some species are alkali-tolerant being inherently present in alkali slag dumps, lake soils and sediments (Schippers et al 2002;Yang et al 2008;Mwirichia et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Nocardiopsis species as potential sources of diverse and novel extracellular enzymes

Bennur

Kumar

Zinjarde

et al. 2014

Appl Microbiol Biotechnol

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Members of the genus Nocardiopsis are generally encountered in locations that are inherently extreme. They are present in frozen soils, desert sand, compost, saline or hypersaline habitats (marine systems, salterns and soils) and alkaline places (slag dumps, lake soils and sediments). In order to survive under these severe conditions, they produce novel and diverse enzymes that allow them to utilize the available nutrients and to thrive. The members of this genus are multifaceted and release an assortment of extracellular hydrolytic enzymes. They produce enzymes that are cold-adapted (α-amylases), thermotolerant (α-amylases and xylanases), thermoalkalotolerant (cellulases, β-1,3-glucanases), alkali-tolerant thermostable (inulinases), acid-stable (keratinase) and alkalophilic (serine proteases). Some of the enzymes derived from Nocardiopsis species act on insoluble polymers such as glucans (pachyman and curdlan), keratin (feathers and prion proteins) and polyhydroxyalkanoates. Extreme tolerance exhibited by proteases has been attributed to the presence of some amino acids (Asn and Pro) in loop structures, relocation of multiple salt bridges to outer regions of the protein or the presence of a distinct polyproline II helix. The range of novel enzymes is projected to increase in the forthcoming years, as new isolates are being continually reported, and the development of processes involving such enzymes is envisaged in the future.

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Section: Introductionmentioning

confidence: 99%

Nocardiopsis species as potential sources of diverse and novel extracellular enzymes

Bennur

Kumar

Zinjarde

et al. 2014

Appl Microbiol Biotechnol

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“…The hemoglobin protease producing strain SCSIO 11720 was isolated from Porites lutea (18°13' N, 109°28' E, Luhuitou fringing reef, Sanya, Hainan province, China) [23]. Coral fragment measuring 2 × 2 cm was airbrushed with sterile seawater in order to obtain tissue slurry.…”

Section: Protease Producing Strain Detectionmentioning

confidence: 99%

Characterization of a thermophilic hemoglobin-degrading protease from Streptomyces rutgersensis SCSIO 11720 and its application in antibacterial peptides production

Yang

et al. 2015

Biotechnol Bioproc E

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High value utilization of byproduct of slaughterhouses is regarded as beneficial to both economy and environment. A hemoglobin degrading protease PA720 was purified from fermentation broth of a Porites lutea associated strain Streptomyces rutgersensis SCSIO 11720 (SCSIO 11720) and exhibited maximal activity at 70°C, pH 10.5. The isolated strain was identified by genotypic method and 16 s RNA gene sequence was deposited in GenBank under accession No. KC414842. The enzyme exhibited excellent thermostability since it was stable at 70°C with t 1/2 value of 1,386.3 min. Kinetic and thermodynamic study showed the activation energy (E a ), ∆G * (free energy), ∆H * (enthalpy) and ∆S * (entropy) for protease deactivation were 62.71 kJ/mol, 74.26 kJ/mol, 59.86 kJ/mol, and -41.97 J/mol/K, respectively. Highest specific activity of protease PA720 was observed when using casein (9,953 U/mg) and hemoglobin (9,854 U/mg) as substrates. Hemoglobin hydrolysate prepared by protease PA720 showed significant antibacterial activity towards Escherichia coli, Staphylococcus aureus and Bacillus subtilis, indicating this protease could be used as an instrumental enzyme for production of hemoglobin-based antibacterial peptides. Based on partial amino acid sequences of the enzyme PA720, the full gene encoding this protease was obtained by degenerate primer PCR and has been deposited in GenBank under accession No. KC414842. The deduced amino acid sequence exhibits homology with other microbial thermophilic proteases in subtilisin family.

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“…marine sediment (Fang et al 2011), gorgonian coral (Li et al 2012), antarctica soil (Xu et al 2014) and feather waste (Saha et al 2013). Many of them prefer moderately alkaline conditions (pH 8.5) (Kroppenstedt 1992) and some grow better on media supplemented with sodium chloride.…”

Section: Introductionmentioning

confidence: 99%

Nocardiopsis mangrovei sp. nov., isolated from mangrove sediment

Huang

Shen

Yuan

et al. 2015

Antonie van Leeuwenhoek

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Two Gram-positive actinobacterial strains, designated HA11166(T) and HA12420, were isolated from mangrove sediments in Hainan, China. The bacterial cells grew with 0-9 % (w/v) NaCl, at 15-40 °C and pH 5.0-10.0, with the optimum growth at 1 % NaCl, 30-37 °C and pH 7.0. The organisms had a range of chemical and morphological properties consistent with their classification in the genus Nocardiopsis. Phylogenetic analysis of the 16S rRNA gene sequences indicated that strains HA11166(T) and HA12420 can be affiliated to the genus Nocardiopsis and most closely related to Nocardiopsis trehalosi VKM Ac-942(T) (with the similarity of 97.2 and 97.5 %, respectively). The value of DNA-DNA relatedness between type strain HA11166(T), selected as the representative strain, and N. trehalosi VKM Ac-942(T) was 38.8 %. The DNA G+C content of strain HA11166(T) was 73.7 %. On the basis of these phenotypic and genotypic data, strains HA11166(T) and HA12420 are proposed to represent a novel species of the genus Nocardiopsis, for which the name Nocardiopsis mangrovei sp. nov. is proposed. The type strain is HA11166(T) (=CGMCC 4.7119(T)=DSM 46665(T)).

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Nocardiopsis coralliicola sp. nov., isolated from the gorgonian coral, Menella praelonga

Cited by 25 publications

References 49 publications

Nocardiopsis species as potential sources of diverse and novel extracellular enzymes

Nocardiopsis species as potential sources of diverse and novel extracellular enzymes

Characterization of a thermophilic hemoglobin-degrading protease from Streptomyces rutgersensis SCSIO 11720 and its application in antibacterial peptides production

Nocardiopsis mangrovei sp. nov., isolated from mangrove sediment

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