Nociceptive-like behavior and analgesia in silver catfish (Rhamdia quelen)

Rodrigues, Patrícia; Barbosa, Luísa Barichello; Bianchini, Adriane Erbice; Ferrari, Fabíola Tonelli; Baldisserotto, Bernardo; Heinzmann, Berta Maria

doi:10.1016/j.physbeh.2019.112648

Cited by 8 publications

(3 citation statements)

References 52 publications

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“…Typically, when introduced in novel conditions, larvae tend to fiercely investigate their surroundings until they acclimate, which is reflected in their locomotor activity [ 26 ]. However, immersion in AA alters this consistent behavioural response, strongly suggesting the induction of a painful stimulus and corroborating previous research [ 27 , 29 , 30 , 31 ]. In contrast, when paracetamol was tested, no alterations in behavioural parameters were observed, confirming its well-established analgesic properties [ 32 ].…”

Section: Discussionsupporting

confidence: 90%

Antinociceptive Analysis of Natural Monoterpenes Eugenol, Menthol, Carvacrol and Thymol in a Zebrafish Larval Model

Rocha,

Félix,

Monteiro

et al. 2024

Pharmaceuticals

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In the last decade, a considerable number of studies have broadened our knowledge of the nociceptive mechanisms of pain, a global health problem in both humans and animals. The use of herbal compounds such as eugenol, menthol, thymol, and carvacrol as analgesic agents has accompanied the growing interest in this area, offering a possible solution for this complex problem. Here, we aimed to explore how these natural substances—at three different concentrations (2, 5 and 10 mg/L)—affect the pain responses in zebrafish (Danio rerio) larvae exposed to 0.05% acetic acid (AA) for 1 min. By analysing the activity of acetylcholinesterase (AChE), 5′-ectonucleotidase and NTPDases, as well as aversion and exploratory behaviours, it was observed that that although all substances were effective in counteracting the pain stimulus, the concentration range within which they do so might be very limited. Eugenol, despite its acknowledged properties in fish anaesthesia, failed to alleviate the pain stimulus at low concentrations. Contrastingly, menthol exhibited the most promising results at the lowest concentrations tested. Overall, it is concluded that menthol might be a good analgesic for this species, qualifying it as a substance of interest for prospective studies.

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Section: Discussionsupporting

confidence: 90%

Antinociceptive Analysis of Natural Monoterpenes Eugenol, Menthol, Carvacrol and Thymol in a Zebrafish Larval Model

Rocha,

Félix,

Monteiro

et al. 2024

Pharmaceuticals

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“…As for physiological roles of this system, opioids are apparently involved in comparable functions to those known in mammals as supported by results obtained in several fish species. These include: anti-nociception in rainbow trout ( Jones et al, 2012 ; Gräns et al, 2014 ) and silver catfish Rhamdia quelen ( Rodrigues et al, 2019 ), analgesia in goldfish ( Kobayashi et al, 2014 ), addiction in zebrafish ( López-Bellido et al, 2012 ; Jimenez-Gonzalez et al, 2016 ), stress response in rainbow trout ( Rosengren et al, 2018 ) and gilthead sea bream ( Albrizio et al, 2014 ), immune response in spotted snakehead ( Singh and Rai, 2009 ), zebrafish ( Liu et al, 2019 ) and northern pike Esox lucius ( Bosi et al, 2020 ), reproduction in goldfish ( Liu et al, 2018 ), oxidative stress in zebrafish ( Liu et al, 2019 ), and aggressive/shy behavior in zebrafish ( Thörnqvist et al, 2019 ). However, in contrast to mammals, there is almost no information in fish regarding the putative role of opioids in food intake regulation ( Bao et al, 2019 ) with few studies demonstrating that β-endorphin stimulates food intake in goldfish ( De Pedro et al, 1995 ) and tench Tinca tinca ( Guijarro et al, 1999 ) apparently through MOP receptor ( De Pedro et al, 1996 ).…”

Section: Introductionmentioning

confidence: 99%

The Opioid System in Rainbow Trout Telencephalon Is Probably Involved in the Hedonic Regulation of Food Intake

et al. 2022

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We hypothesize that opioids are involved in the regulation of food intake in fish through homeostatic and hedonic mechanisms. Therefore, we evaluated in rainbow trout (Oncorhynchus mykiss) hypothalamus and telencephalon changes in precursors, endogenous ligands and receptors of the opioid system under different situations aimed to induce changes in the homeostatic (through fasted/fed/refed fish) and hedonic (through feeding fish a control or a palatable high-fat diet) regulation of food intake. No major changes occurred in parameters assessed related with the nutritional condition of fish (fasted/fed/refed), allowing us to suggest that the opioid system seems not to have an important role in the homeostatic regulation of food intake in rainbow trout. The responses observed in telencephalon of rainbow trout fed the palatable high-fat diet included a decrease in mRNA abundance of the opioid precursor penka, in a way similar to that known in mammals, and increased mRNA abundance of the opioid receptors oprd1 and oprk1 supporting a role for telencephalic opioid system in the hedonic regulation of food intake in fish.

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“…Given the importance of movement tracking of laboratory animals, numerous open-source programs and commercial systems have been developed for recording and analyzing animal behavior, such as Limelight (Actimetrics, USA) (Jimenez et al, 2018;Ishii et al, 2019;Takemoto and Song, 2019), ANY-maze (Stoelting Co, USA) (Morin and Studholme, 2011;Rodrigues et al, 2019;Feng et al, 2020;Scarsi et al, 2020), Ethovision R XT (Noldus, The Netherlands) (Noldus et al, 2001;Yu et al, 2020), TopScan (Clever Sys Inc., USA) (Grech et al, 2019;Griffiths et al, 2019), Super-maze (Shanghai Xinruan information Technology Co., China) (Hao et al, 2012;Qiao et al, 2017), and others (Samson et al, 2015;Gulyás et al, 2016;Bello-Arroyo et al, 2018;Hewitt et al, 2018). In previous studies, motion tracking in videos captured by the video camera is the most common and low-cost approach to achieve tracking of multiple parameters.…”

Section: Introductionmentioning

confidence: 99%

DeepBhvTracking: A Novel Behavior Tracking Method for Laboratory Animals Based on Deep Learning

Sun

Lyu

Cai

et al. 2021

Front. Behav. Neurosci.

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Behavioral measurement and evaluation are broadly used to understand brain functions in neuroscience, especially for investigations of movement disorders, social deficits, and mental diseases. Numerous commercial software and open-source programs have been developed for tracking the movement of laboratory animals, allowing animal behavior to be analyzed digitally. In vivo optical imaging and electrophysiological recording in freely behaving animals are now widely used to understand neural functions in circuits. However, it is always a challenge to accurately track the movement of an animal under certain complex conditions due to uneven environment illumination, variations in animal models, and interference from recording devices and experimenters. To overcome these challenges, we have developed a strategy to track the movement of an animal by combining a deep learning technique, the You Only Look Once (YOLO) algorithm, with a background subtraction algorithm, a method we label DeepBhvTracking. In our method, we first train the detector using manually labeled images and a pretrained deep-learning neural network combined with YOLO, then generate bounding boxes of the targets using the trained detector, and finally track the center of the targets by calculating their centroid in the bounding box using background subtraction. Using DeepBhvTracking, the movement of animals can be tracked accurately in complex environments and can be used in different behavior paradigms and for different animal models. Therefore, DeepBhvTracking can be broadly used in studies of neuroscience, medicine, and machine learning algorithms.

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Nociceptive-like behavior and analgesia in silver catfish (Rhamdia quelen)

Cited by 8 publications

References 52 publications

Antinociceptive Analysis of Natural Monoterpenes Eugenol, Menthol, Carvacrol and Thymol in a Zebrafish Larval Model

Antinociceptive Analysis of Natural Monoterpenes Eugenol, Menthol, Carvacrol and Thymol in a Zebrafish Larval Model

The Opioid System in Rainbow Trout Telencephalon Is Probably Involved in the Hedonic Regulation of Food Intake

DeepBhvTracking: A Novel Behavior Tracking Method for Laboratory Animals Based on Deep Learning

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