Nodax™ Class PHA Copolymers: Their Properties and Applications

Noda, Isao; Lindsey, S. Blake; Caraway, Daniel

doi:10.1007/978-3-642-03287-5_10

Cited by 53 publications

(35 citation statements)

References 18 publications

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“…The monomer composition of PHA polymers greatly influences their physical and thermal properties [8,9]. Unlike the polymers produced by P. putida LS46, polymers produced by P. putida LS46123 on different substrates were semisolid.…”

Section: Discussionmentioning

confidence: 99%

Synthesis and Physical Properties of Polyhydroxyalkanoate Polymers with Different Monomer Compositions by Recombinant Pseudomonas putida LS46 Expressing a Novel PHA SYNTHASE (PhaC116) Enzyme

et al. 2017

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A recombinant of Pseudomonas putida LS461 (deletion of the phaC1phaZphaC2 genes) was constructed by introducing cosmid JC123 carrying a novel phaC1 16 gene from a metagenomic clone. The resulting strain, P. putida LS46123, was able to synthesize polyhydroxyalkanoate (PHA) polymers with novel monomer compositions when cultured on glucose or free fatty acids, and accumulated PHAs from 9.24% to 27.09% of cell dry weight. The PHAs synthesized by P. putida LS46123 contained up to 50 mol % short chain length subunits (3-hydroxybutyrate and 3-hydroxyvalerate), with the remaining monomers consisting of various medium chain length subunits. The PhaC1 16 protein expressed by P. putida LS46123 had an amino acid sequence similarity of 45% with the PhaC1 protein of the parent strain, P. putida LS46. Predicted 3D structures of the PhaC1 16 proteins from P. putida LS46123 and P. putida LS46 revealed several differences in the numbers and locations of protein secondary structures. The physical and thermal properties of the novel polymers synthesized by P. putida LS46123 cultured with glucose or free fatty acids differed significantly from those produced by P. putida LS46 grown on the same substrates. PHA polymers with different subunit compositions, and hence different physical and thermal properties, can be tailor-made using novel PHA synthase for specific applications.

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Section: Discussionmentioning

confidence: 99%

Synthesis and Physical Properties of Polyhydroxyalkanoate Polymers with Different Monomer Compositions by Recombinant Pseudomonas putida LS46 Expressing a Novel PHA SYNTHASE (PhaC116) Enzyme

et al. 2017

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“…A successful example is the PHBHHx that was produced on an industrial scale (Chen 2009b). US-based Procter & Gamble has trademarked scl and mcl PHA copolymers of C 4 and C 6 -C 12 as Nodax TM (Noda et al 2009). …”

Section: Copolymer Phamentioning

confidence: 99%

Plastics Completely Synthesized by Bacteria: Polyhydroxyalkanoates

Chen

2009

Microbiology Monographs

178

106

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Polyhydroxyalkanoates (PHA) produced by many bacteria have been investigated by microbiologists, molecular biologists, biochemists, chemical engineers, chemists, polymer experts, and medical researchers over the past many years. Applications of PHA as bioplastics, fine chemicals, implant biomaterials, medicines, and biofuels have been developed. Companies have been established or involved in PHA-related R&D as well as large-scale production. PHA synthesis has been found to improve the robustness of non-PHA-producing microorganisms and to regulate bacterial metabolism, leading to yield improvement for some bacterial fermentation products. In addition, amphiphilic proteins related to PHA synthesis including PhaP, PhaZ, and PhaC have been found to be useful for achieving protein

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“…The incorporation of a fraction of 3HV (or other monomers with a chain length from 3 to 14 carbon atoms) in the PHB backbone significantly increases the softness and flexibility of the biopolymer (Noda et al 2010), increases thermal stability (Zakaria et al 2010) and lowers the melting point (McChalicher and Srienc 2007). PHA copolymers, such as PHBV, are therefore often preferred for practical use, and a range of such materials has been available as commercial commodities (Dai et al 2008).…”

Section: Materials Propertiesmentioning

confidence: 99%

“…Although many types of PHA have been discovered (Figure 2.1), the homopolymer poly-3-hydroxybutyrate (PHB) is the best known and characterised (Lee 1996). Only a few types of PHAs have been produced at large scale, these being PHB and the copolymers of 3-hydroxybutyrate with 3-hydroxyvalerate (poly(3-hydroxybutyrate-co-3-hydroxyvalerate, PHBV) and with 3-hydroxyhexanoate (poly(3-hydroxybutyrate-co-3-hydrohexanoate, PHBHHx) (Noda et al 2010). …”

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confidence: 99%

“…Poly(hydroxyalkanoates) (PHAs), while presenting the benefit of being both bio-based and biodegradable polymers, have two disadvantages that may currently limit wider industrial scale applications in the polymer processing industry: their price is still high compared to commodity petroleum-based polymers, and the most widespread PHA polymer, PHB is a brittle material with high melting temperature (T m ) and crystallinity (Noda et al 2010). For PHB related copolymers such as PHBV containing low 3HV monomer content (less than 25%), PHB properties dominate, which limits the processing window and end-use applications (McChalicher and Srienc 2007).…”

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confidence: 99%

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Composition of mixed culture PHA biopolymers and implications for downstream processing

Montano-Herrera¹

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Polyhydroxyalkanoates (PHAs) are biopolymers synthesised and accumulated by most bacteria for carbon and energy storage. They have properties and applications comparable to petrochemical thermoplastics. Although PHAs are produced at high yields using pure biological cultures, the use of mixed cultures can significantly reduce the production costs and make use of waste streams to produce environmentally sustainable materials. In order to produce mixed culture PHAs of relevance for broader industry applications it is necessary to develop the ability to tailor polymers with diversified mechanical properties through understanding and controlling the monomer composition and compositional distribution.Diverse and complex PHA polymer structures have been achieved in mixed microbial cultures using time-based feeding strategies. However, PHA monomer composition and compositional distribution in PHA random and blocky copolymers are sensitive to substrate feeding history, making more complex the prediction of final PHA content and composition.In this sense, a better understanding of the changing cell physiologies that develop in response to different feeding strategies and substrates is necessary to design optimised feeding strategies and process control algorithms for PHA production.This thesis presents for the first time a comprehensive flux characterisation of monomer development during mixed culture PHA accumulation concurrent with biomass growth using Metabolic Flux Analysis (MFA). A dynamic trend in active biomass growth and in polymer composition was observed and was consistent over replicate accumulations. Monomer (3-hydroxybutyrate and 3-hydroxyvalerate) incorporation into poly(3-hydroxybutyate-co-3-hydroxyvalerate) copolymers in a pilot scale production system was evaluated based on published models that describe polymer production during PHA accumulation. However, it was found that these existing models could not describe the fluctuations in the proportion of Additionally, thermal degradation of mixed culture PHAs during melt processing was assessed by Near-Infrared (NIR) spectroscopy coupled to Multivariate Data Analysis. It was shown that, with correct pretreatment, a copolymeric product that was much more stable to extrusion processing than commercially available PHA was produced.Overall, this thesis explores both the fundamental and applied aspects of PHA production by mixed microbial cultures with concurrent active biomass growth. The use of computational iii tools to explore and help understand the underlying metabolic processes was explored.Polymer composition seems to follow a very complex regulation processes which can be described through the incorporation of more detailed reactions in current metabolic models.Furthermore, this thesis gives further insight into the fundamental properties of the materials produced and an assessment of their potential for degradation during processing.iv

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Nodax™ Class PHA Copolymers: Their Properties and Applications

Cited by 53 publications

References 18 publications

Synthesis and Physical Properties of Polyhydroxyalkanoate Polymers with Different Monomer Compositions by Recombinant Pseudomonas putida LS46 Expressing a Novel PHA SYNTHASE (PhaC116) Enzyme

Synthesis and Physical Properties of Polyhydroxyalkanoate Polymers with Different Monomer Compositions by Recombinant Pseudomonas putida LS46 Expressing a Novel PHA SYNTHASE (PhaC116) Enzyme

Plastics Completely Synthesized by Bacteria: Polyhydroxyalkanoates

Composition of mixed culture PHA biopolymers and implications for downstream processing

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