Nodulation and nitrogen fixation by fast- and slow-growing rhizobia strains of soybean on several temperate and tropical legumes

Chamber, Manuel; Iruthayathas, E. E.

doi:10.1007/bf02140001

Cited by 18 publications

(6 citation statements)

References 23 publications

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“…Numerous other studies have also shown that soybeans inoculated with USDA110 showed greater N 2 fixation, which supports increased biomass accumulation (Schubert et al . , ; Chamber & Iruthayathas ; Lynch & Smith ; Soe et al . ).…”

Section: Discussionmentioning

confidence: 99%

Inoculation with an enhanced N₂‐fixing Bradyrhizobium japonicum strain (USDA110) does not alter soybean (Glycine max Merr.) response to elevated [CO₂]

Sanz‐Sáez

Heath

Burke

et al. 2015

Plant Cell & Environment

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This study tested the hypothesis that inoculation of soybean (Glycine max Merr.) with a Bradyrhizobium japonicum strain (USDA110) with greater N2 fixation rates would enhance soybean response to elevated [CO2 ]. In field experiments at the Soybean Free Air CO2 Enrichment facility, inoculation of soybean with USDA110 increased nodule occupancy from 5% in native soil to 54% in elevated [CO2 ] and 34% at ambient [CO2 ]. Despite this success, inoculation with USDA110 did not result in greater photosynthesis, growth or seed yield at ambient or elevated [CO2 ] in the field, presumably due to competition from native rhizobia. In a growth chamber experiment designed to study the effects of inoculation in the absence of competition, inoculation with USDA110 in sterilized soil resulted in nodule occupation of >90%, significantly greater (15) N2 fixation, photosynthetic capacity, leaf N and total plant biomass compared with plants grown with native soil bacteria. However, there was no interaction of rhizobium fertilization with elevated [CO2 ]; inoculation with USDA110 was equally beneficial at ambient and elevated [CO2 ]. These results suggest that selected rhizobia could potentially stimulate soybean yield in soils with little or no history of prior soybean production, but that better quality rhizobia do not enhance soybean responses to elevated [CO2 ].

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Section: Discussionmentioning

confidence: 99%

Inoculation with an enhanced N₂‐fixing Bradyrhizobium japonicum strain (USDA110) does not alter soybean (Glycine max Merr.) response to elevated [CO₂]

Sanz‐Sáez

Heath

Burke

et al. 2015

Plant Cell & Environment

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“…Strains related to USDA110 are found to nodulate cowpea in Africa (Pule-Meulenberg et al 2010). Most cowpea cultivars respond positively to USDA110 inoculation (Keyser et al 1982), and it provides substantial nitrogen fixation to cowpeas compared with other rhizobial strains (Yelton et al 1983;Chamber and Iruthayathas 1988). USDA110-ARS (hereafter, Fix+) is a spontaneous mutant of USDA110 arising from antibiotic selection on azide (10 μg mL −1 ), rifampicin (500 μg mL −1 ), and streptomycin (1000 μg mL −1 ; Kuykendall and Weber 1978) that was confirmed to efficiently fix nitrogen on six genotypes of soybeans (Kiers et al 2007).…”

Section: Cowpea Genotypesmentioning

confidence: 99%

“…Bradyrhizobium STRAINS USDA110 was isolated from soybean in the United States (Kaneko et al 2002) and is a broadly used inoculant for legume crops (Keyser et al 1982;Chamber and Iruthayathas 1988;Urtz and Elkan 1996;Musiyiwa et al 2005). Strains related to USDA110 are found to nodulate cowpea in Africa (Pule-Meulenberg et al 2010).…”

Section: Cowpea Genotypesmentioning

confidence: 99%

No disruption of rhizobial symbiosis during early stages of cowpea domestication

et al. 2022

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Modern agriculture intensely selects aboveground plant structures, while often neglecting belowground features, and evolutionary tradeoffs between these traits are predicted to disrupt host control over microbiota. Moreover, drift, inbreeding, and relaxed selection for symbiosis in crops might degrade plant mechanisms that support beneficial microbes. We studied the impact of domestication on the nitrogen-fixing symbiosis between cowpea and root-nodulating Bradyrhizobium. We combined genome-wide analyses with a greenhouse inoculation study to investigate genomic diversity, heritability, and symbiosis trait variation among wild and early-domesticated cowpea genotypes. Cowpeas experienced modest decreases in genome-wide diversity during early domestication. Nonetheless, domesticated cowpeas responded efficiently to variation in symbiotic effectiveness, by forming more root nodules with nitrogen-fixing rhizobia and sanctioning nonfixing strains. Domesticated populations invested a larger proportion of host tissues into root nodules than wild cowpeas. Unlike soybean and wheat, cowpea showed no compelling evidence for degradation of symbiosis during domestication. Domesticated cowpeas experienced a less severe bottleneck than these crops and the low nutrient conditions in Africa where cowpea landraces were developed likely favored plant genotypes that gain substantial benefits from symbiosis. Breeders have largely neglected symbiosis traits, but artificial selection for improved plant responses to microbiota could increase plant performance and sustainability.

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“…1994;Young 1996). However, strains were found to show a broad host-range and formed nodules on soybean as well as many other legumes, including cowpea, pigeon pea and mungbean (Scholla and Elkan 1984;Stowers and Eaglesham 1984;Chamber and Iruthayathas 1988). This contrasts with the restricted nodulation properties of strain J-TGS50 which did not nodulate mungbean.…”

Section: Escherichia Colimentioning

confidence: 99%

Characterization and Phylogenetic Analysis of Soybean Rhizobial Strains from Java and Sumatra

Waluyo

2011

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Twenty-seven and twenty-four soybean rhizobial isolates from Java and Sumatra, respectively,were characterized. Based on cross-inoculation, eight isolates from Java and nine from Sumatra could be grouped as soybean specific rhizobial species , while 19 isolates from Java and 15 from Sumatra were promiscuous. ARDRA of intergenic spacer region of 16S-23S rDNA showed that the isolates from Java were different from those from Sumatra. Six soybean specific isolates from Java and one from Sumatra were in the same cluster with the reference strain, Bradyrhizobium japonicum USDA 110, thus could be classified as B. japonicum. One soybean specific isolate from Java’s has a distinct position, while the other soybean specific isolate from Java was placed in another group dominated by isolates from Sumatra. The nineteen promiscuous isolates from Java were clustered in a different group. This group, together with the isolate with distinct position and the other group that were dominated by isolates from Sumatra, were distinct from B. japonicum USDA 110. Therefore it is tempting to speculate that they represent indigenous soybean rhizobial strains. Based on complete sequencing of the amplified 16S rDNA of 21 selected isolates, these isolates could be divided into three groups consisting of twelve Bradyrhizobium elkanii, eight Bradyrizobium japonicum and one Sinorhizobium fredii. Most of the B. elkanii strains were isolated from acid soils at Sitiung, West-Sumatra, while only two isolates were obtained from Java. Four isolates from Java, two isolates from Sitiung, and two isolates from Bukit Tinggi were identified as B. japonicum. One isolate from Java with a distinct position on the ARDRA was identified as S. fredii

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Nodulation and nitrogen fixation by fast- and slow-growing rhizobia strains of soybean on several temperate and tropical legumes

Cited by 18 publications

References 23 publications

Inoculation with an enhanced N₂‐fixing Bradyrhizobium japonicum strain (USDA110) does not alter soybean (Glycine max Merr.) response to elevated [CO₂]

Inoculation with an enhanced N₂‐fixing Bradyrhizobium japonicum strain (USDA110) does not alter soybean (Glycine max Merr.) response to elevated [CO₂]

No disruption of rhizobial symbiosis during early stages of cowpea domestication

Characterization and Phylogenetic Analysis of Soybean Rhizobial Strains from Java and Sumatra

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Nodulation and nitrogen fixation by fast- and slow-growing rhizobia strains of soybean on several temperate and tropical legumes

Cited by 18 publications

References 23 publications

Inoculation with an enhanced N2‐fixing Bradyrhizobium japonicum strain (USDA110) does not alter soybean (Glycine max Merr.) response to elevated [CO2]

Inoculation with an enhanced N2‐fixing Bradyrhizobium japonicum strain (USDA110) does not alter soybean (Glycine max Merr.) response to elevated [CO2]

No disruption of rhizobial symbiosis during early stages of cowpea domestication

Characterization and Phylogenetic Analysis of Soybean Rhizobial Strains from Java and Sumatra

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Product

Resources

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Inoculation with an enhanced N₂‐fixing Bradyrhizobium japonicum strain (USDA110) does not alter soybean (Glycine max Merr.) response to elevated [CO₂]

Inoculation with an enhanced N₂‐fixing Bradyrhizobium japonicum strain (USDA110) does not alter soybean (Glycine max Merr.) response to elevated [CO₂]