Nodulation, nitrogen uptake and growth of lima bean in a composted tannery sludge-treated soil

Abstract

This study evaluated the responses of lima bean (Phaseolus lunatus L.) to application of composted tannery sludge on nodulation, N uptake and plant growth. For eight years, the compost was applied at rates of: 0, 2.5, 5, 10, and 20 Mg ha-1 (dry basis). Plants of lima bean showed higher nodulation in treatments with compost; however, nodules reported in these treatments presented lower biomass, size and diversity than those reported in treatment without compost. Accumulation of N increased with the application of the highest rate (20 Mg ha-1), while there was an increase in chromium content in shoot with the increase in compost rates. Thus, the use of composted tannery sludge, in long-term, increases the accumulation of chromium in plants, increasing nodulation, while decrease rhizobia diversity in nodules.

Inoculation of rhizobia increases lima bean (Phaseolus lunatus) yield in soils from Piauí and Ceará states, Brazil

“…Afterward, nodules, shoots and roots were dried (65°C; 72 h) and weighed to determine nodules (NDW), shoot (SDW) and roots (RDW) dry weight. Nodules size (NS) was estimated by using the ratio between NDW and NN, expressed as mg per nodule (Rocha et al, 2019). Total N in plant was estimated by by Kjedahl method.…”

Diversity and biotechnological potential of rhizobia isolated from lima bean nodules collected at a semiarid region

“…Although lima bean has the important characteristic of establishing symbiosis with rhizobia (Araujo et al, 2015;Chibeba et al, 2020;Matsubara & Zúñiga-Dávila, 2015;Ormeño-Orrillo et al, 2006), the phenotypic and genotypic diversity and the symbiotic efficiency of rhizobia isolated from the nodules of this leguminous plant have not been widely studied. Only eight studies worldwide have genetically characterized isolates from lima bean nodules (Araujo et al, 2015;Chibeba et al, 2020;Durán et al, 2014;López-López et al, 2013;Matsubara & Zúñiga-Dávila, 2015;Ormeño-Orrillo et al, 2006;Rocha et al, 2020;Santos et al, 2011), and in Brazil, there have been only nine studies in relation to symbiosis between rhizobia and lima bean (Antunes et al, 2011;Araujo et al, 2015;Costa Amorim et al, 2019;Costa et al, 2021;Cubillos-Hinojosa et al, 2021;Neto et al, 2017;Rocha et al, 2019;Santos et al, 2011). However, only a few of these papers evaluated the efficiency of strains in symbiosis with lima bean.…”