Nodulation of rooibos (Aspalathus linearis Burm. f.), an indigenous South African legume, by members of both the α-Proteobacteria and β-Proteobacteria

Hassen, Ahmed Idris; Bopape, Francina L.; Habig, Johannes H; Lamprecht, S. C.

doi:10.1007/s00374-011-0628-3

Cited by 42 publications

(30 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The endogenous soil factors mentioned here could account for the increased N contribution made by Bambara groundnut plants at those sites (Table 4). However, the differences in symbiotic performance by Bambara groundnut could also be due to its bacterial partner as legumes growing in nutrient-poor, low pH soils in Africa have been found to form root nodules with both α-and β-proteobacteria (Hassen et al 2012;Kanu and Dakora 2012;Angus et al 2013;Howieson et al 2013). It is important to note that even though Bambara groundnut plants derived as high as 98 % of their N nutrition from symbiotic fixation at Malekutu (field 12), the actual amount of N-fixed was very low, only 4 kg ha −1 (Table 4).…”

Section: Symbiotic N Nutrition By Bambara Groundnut In Farmers' Fieldsmentioning

confidence: 99%

Symbiotic N nutrition, C assimilation, and plant water use efficiency in Bambara groundnut (Vigna subterranea L. Verdc) grown in farmers’ fields in South Africa, measured using 15N and 13C natural abundance

2013

View full text Add to dashboard Cite

Bambara groundnut (Vigna subterranea L. Verdc) is the second most important indigenous food legume in Africa. The aim of this study was to evaluate plant growth, N 2 fixation, N contribution, C accumulation, and plant water relations of Bambara groundnut grown in 26 farmers' fields in Mpumalanga Province of South Africa. The data revealed marked (p ≤0.05) differences in plant dry matter (DM) yield, N concentration and content, δ

show abstract

Section: Symbiotic N Nutrition By Bambara Groundnut In Farmers' Fieldsmentioning

confidence: 99%

Symbiotic N nutrition, C assimilation, and plant water use efficiency in Bambara groundnut (Vigna subterranea L. Verdc) grown in farmers’ fields in South Africa, measured using 15N and 13C natural abundance

2013

View full text Add to dashboard Cite

show abstract

“…Moreover, a number of rare Bradyrhizobium taxa were also characteristic of particular community types, in particular those in the Skimmelberg mountain range. The role of Mesorhizobium as the core rhizobial symbionts of rooibos has been reported a number of times before (Hassen et al, 2012;Lemaire et al, 2015;Le Roux et al, 2017;Ramoneda et al, 2020), and the genus is considered generalist in terms of soil environmental tolerance, particularly towards pH (Dludlu et al, 2018). While edaphic factors (e.g.…”

Section: Discussionmentioning

confidence: 99%

“…an ecological insurance strategy, Yachi and Loreau, 1999; but see Werner et al, 2018). Examples of this include invasive species such as Australian acacias (Ndlovu et al, 2013;Keet et al, 2017;Le Roux et al, 2018;Dinnage et al, 2019), South African legumes in nutrient-poor soils (Hassen et al, 2012;Lemaire et al, 2015;Le Roux et al, 2017) and legumes in semi-arid regions of Brazil (Radl et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…Second, the species contains distinct ecotypes in the wild (Hawkins et al, 2011), often sympatric with plantations and in a highly heterogeneous landscape where physical barriers to dispersal are common (Cowling et al, 2009;Dludlu et al, 2017). Lastly, recent studies have found rooibos to associate with a wide diversity of rhizobia (Hassen et al, 2012;Le Roux et al, 2017;Ramoneda et al, 2020), particularly at the seedling stage. While the species has a preference for Mesorhizobium strains, it has weak control over its rhizobial partners.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Ecological patterns of root nodule diversity in cultivated and wild rooibos populations: a community prediction approach

Ramoneda

Roux

Frossard

et al. 2020

Preprint

View full text Add to dashboard Cite

There is interest in understanding the factors behind the biogeography of root-associated bacteria due to the joint effects that plant host, climate, and soil conditions can have on bacterial diversity. For legume crops with remaining wild populations, this is of even more importance, because the effects of cropping on undisturbed root-associated bacterial communities can be addressed. Here, we used a community prediction approach to describe the diversity of the root nodule bacterial communities of rooibos (Aspalathus linearis), an endemic legume crop from South Africa. The goal was to reveal whether patterns of root nodule community composition in paired cultivated and wild rooibos populations could be related to geographical distance, plant traits, and plant population type (i.e. cultivated or uncultivated). We identified a core of dominant and widespread Mesorhizobium ZOTUs that each defined one of 4 different root nodule community classes. Rooibos cultivation impacted root nodule bacterial diversity at regional and local scales, while the geographical origin of the root nodule communities was the strongest predictor of root nodule community structure. Beyond impacts of cultivation on root nodule bacterial diversity, this study suggests a mixture of dispersal limitation and ecological drift regionally, and selection by different plant populations locally, define the biogeography of rooibos root nodule bacterial communities.

show abstract

“…Aspalathus linearis (burm f.), commonly known by the name rooibos, is an economically important beverage legume endemic to the Cape floristic region in South Africa (Hassen et al 2012). Like in many other legumes, the presence of both the symbiotic root nodule rhizobia and the free-living indigenous soil microbes in the soil plays crucial roles in growth and normal functioning of this legume crop.…”

Section: Introductionmentioning

confidence: 99%

Assessing root nodule microsymbionts in healthy and declined rooibos (Aspalathus linearis burm f.) at a plantation in South Africa

Hassen¹,

Habig²,

Lamprecht³

2018

Journal of Plant Interactions

Self Cite

View full text Add to dashboard Cite

Aspalathus linearis (burm f.), commonly known as rooibos, grows in nutrient and organic matter poor sandy soils that limit its growth. In this study, samples of nodules from both declined and healthy rooibos plants were collected to determine the frequency of nodule nitrogen-fixing and endophytic bacteria. Standard microbiological procedures as well as sequence analysis of the 16S rRNA revealed that more than 75% of the bacterial isolates from the healthy plants contained microsymbionts belonging to the Rhizobium group and the remaining 25% were characterized as Pseudomonas and Burkholderia spp. The nodule from the declined plants lacks a sufficient number of rhizobia and was mostly white in color, small and contains the free-living endospore-forming Bacillus and other endophytic Burkholderia and Pseudomonas spp. The results provide a baseline data on the microsymbionts of rooibos nodules in Citrusdal and highlighted the need for further investigation using additional techniques. ARTICLE HISTORY

show abstract

Nodulation of rooibos (Aspalathus linearis Burm. f.), an indigenous South African legume, by members of both the α-Proteobacteria and β-Proteobacteria

Cited by 42 publications

References 26 publications

Symbiotic N nutrition, C assimilation, and plant water use efficiency in Bambara groundnut (Vigna subterranea L. Verdc) grown in farmers’ fields in South Africa, measured using 15N and 13C natural abundance

Symbiotic N nutrition, C assimilation, and plant water use efficiency in Bambara groundnut (Vigna subterranea L. Verdc) grown in farmers’ fields in South Africa, measured using 15N and 13C natural abundance

Ecological patterns of root nodule diversity in cultivated and wild rooibos populations: a community prediction approach

Assessing root nodule microsymbionts in healthy and declined rooibos (Aspalathus linearis burm f.) at a plantation in South Africa

Contact Info

Product

Resources

About