Nodule formation and mineralisation of human primary osteoblasts cultured on a porous bioactive glass scaffold

Gough, Julie E.; Jones, Julian R.; Hench, Larry L.

doi:10.1016/j.biomaterials.2003.07.001

Cited by 402 publications

(308 citation statements)

References 29 publications

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“…Figure 6a shows a cross-section of the scaffold obtained, in which the presence of a small number of macropores and diffuse microporosity may be seen. Microporosity is a key factor for nutrient flow inside the scaffold, and is also known to play a positive role on proteins and cellular adhesion [20]. Figure 6b shows the scaffold at higher magnification; the high degree of sintering of the pore struts and the pore interconnection can be observed.…”

Section: Resultsmentioning

confidence: 97%

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Biocompatible glass–ceramic materials for bone substitution

Brovarone

Verné

Robiglio

et al. 2007

J Mater Sci: Mater Med

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A new bioactive glass composition (CEL2) in the SiO 2 -P 2 O 5 -CaO-MgO-K 2 O-Na 2 O system was tailored to control pH variations due to ion leaching phenomena when the glass is in contact with physiological fluids. CEL2 was prepared by a traditional meltingquenching process obtaining slices that were heat-treated to obtain a glass-ceramic material (CEL2GC) that was characterized thorough SEM analysis. Pre-treatment of CEL2GC with SBF was found to enhance its biocompatibility, as assessed by in vitro tests. CEL2 powder was then used to synthesize macroporous glass-ceramic scaffolds. To this end, CEL2 powders were mixed with polyethylene particles within the 300-600 lm size-range and then pressed to obtain crack-free compacted powders (green). This was heat-treated to remove the organic phase and to sinter the inorganic phase, leaving a porous structure. The biomaterial thus obtained was characterized by X-ray diffraction, SEM equipped with EDS, density measurement, image analysis, mechanical testing and in vitro evaluation, and found to be a glass-ceramic macroporous scaffold with uniformly distributed and highly interconnected porosity. The extent and size-range of the porosity can be tailored by varying the amount and size of the polyethylene particles.

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Section: Resultsmentioning

confidence: 97%

“…Specifically, these biomaterials have found clinical applications as coating for prostheses, bone filler, vertebral substitution and, in a porous form, as bone substitutes [5][6][7][8][9][10][11][12]. Most of them are based on the SiO 2 -P 2 O 5 -CaO-Na 2 O system.…”

Section: Introductionmentioning

confidence: 99%

Biocompatible glass–ceramic materials for bone substitution

Brovarone

Verné

Robiglio

et al. 2007

J Mater Sci: Mater Med

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“…Indeed, numerous in vivo and in vitro studies have shown that 45S5 Bioglass ® can stimulate bone regeneration (Bosetti et al 2003;Gough et al 2004;Livingston et al 2002;Loty et al 2001;Tsigkou et al 2007;Xynos et al 2000). As a result, Bioglass ® is said to be both osteoproductive and osteoconductive.…”

Section: Introductionmentioning

confidence: 99%

Bone tissue engineering by using a combination of polymer/Bioglass composites with human adipose-derived stem cells

Kirkham

et al. 2014

Cell Tissue Res

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Translational research in bone tissue engineering is essential for 'bench to bedside' patient benefit. However, the idea combination of stem cells and biomaterial scaffolds for bone repair/regeneration is still unclear. The aim of this study was to investigate the osteogenic capacity of a combination of poly(DL-lactic acid) (PDLLA) porous foams containing 5 and 40 wt% of Bioglass ® particles with human adipose-derived stem cells (ADSCs) in vitro and in vivo. Live/dead fluorescent markers, confocal microscopy and scanning electron microscopy (SEM) showed that PDLLA/Bioglass ® porous scaffolds supported ADSCs attachment, growth and osteogenic differentiation, which were confirmed by enhanced alkaline phosphatase activity (ALP). Higher Bioglass ® content of the PDLLA foams increased more ALP activity compared to PDLLA only group. Extracellular matrix deposition after 8 weeks in in vitro culture was evident by Alcian blue/Sirius red staining.In vivo bone formation was assessed using scaffold/ADSCs constructs in diffusion chambers transplanted intraperitoneally into nude mice and recovered after 8 weeks.Histological and immunohistochemical assays indicated significant new bone formation in the 40 wt% and 5 wt% Bioglass ® constructs compared to the PDLLA only group. This study indicated the combination of a well-developed biodegradable bioactive porous PDLLA/Bioglass ® composite scaffold with a high potential stem cells source -human ADSCs could be a promising approach for bone regeneration in clinical setting.

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“…The use of human cells is another advantage—here an MG63 osteoblast‐like cell line (Chang et al, 2014; Gittens et al, 2011; Madhankumar et al, 2014; Tan et al, 2011; Yazici et al, 2013; Yeung et al, 2013) and primary human osteoblast cells (Balani et al, 2007; Gough et al, 2004; Jones et al, 2007; Rice et al, 2003) were used. Although the MG63 cell line is immortalized (Schmidt et al, 2001), such cells are suitable for establishing initial experimental conditions.…”

Section: Discussionmentioning

confidence: 99%

Combining 3D human in vitro methods for a 3Rs evaluation of novel titanium surfaces in orthopaedic applications

Stevenson

Rehman

Draper

et al. 2016

Biotech & Bioengineering

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In this study, we report on a group of complementary human osteoblast in vitro test methods for the preclinical evaluation of 3D porous titanium surfaces. The surfaces were prepared by additive manufacturing (electron beam melting [EBM]) and plasma spraying, allowing the creation of complex lattice surface geometries. Physical properties of the surfaces were characterized by SEM and profilometry and 3D in vitro cell culture using human osteoblasts. Primary human osteoblast cells were found to elicit greater differences between titanium sample surfaces than an MG63 osteoblast‐like cell line, particularly in terms of cell survival. Surface morphology was associated with higher osteoblast metabolic activity and mineralization on rougher titanium plasma spray coated surfaces than smoother surfaces. Differences in osteoblast survival and metabolic activity on titanium lattice structures were also found, despite analogous surface morphology at the cellular level. 3D confocal microscopy identified osteoblast organization within complex titanium surface geometries, adhesion, spreading, and alignment to the biomaterial strut geometries. Mineralized nodule formation throughout the lattice structures was also observed, and indicative of early markers of bone in‐growth on such materials. Testing methods such as those presented are not traditionally considered by medical device manufacturers, but we suggest have value as an increasingly vital tool in efficiently translating pre‐clinical studies, especially in balance with current regulatory practice, commercial demands, the 3Rs, and the relative merits of in vitro and in vivo studies. Biotechnol. Bioeng. 2016;113: 1586–1599. © 2015 The Authors. Biotechnology and Bioengineering Published by Wiley Periodicals, Inc.

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Nodule formation and mineralisation of human primary osteoblasts cultured on a porous bioactive glass scaffold

Cited by 402 publications

References 29 publications

Biocompatible glass–ceramic materials for bone substitution

Biocompatible glass–ceramic materials for bone substitution

Bone tissue engineering by using a combination of polymer/Bioglass composites with human adipose-derived stem cells

Combining 3D human in vitro methods for a 3Rs evaluation of novel titanium surfaces in orthopaedic applications

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