Nodulin Intrinsic Proteins: Facilitators of Water and Ammonia Transport across the Symbiosome Membrane

Routray, Pratyush; Masalkar, Pintu D.; Roberts, Daniel M.

doi:10.1002/9781119053095.ch69

Cited by 6 publications

(2 citation statements)

References 49 publications

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“…When seeds begin to germinate, they require the transportation of large amounts of compounds, such as water and nutrients, which are needed for the synthesis of numerous components. Transporters allow higher plants to uptake an abundance of compounds necessary for cellular component synthesis across specific cell membranes during germination (Routray et al, 2015). ABC transporters, for example, are critical for plant development, as they are involved in seed development, seed germination and secondary growth (Bruhn et al, 2020;Cai et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Proteomic characterization of the different stages of seed germination in Cupressus gigantea

Li,

Zhang,

Lei

et al. 2024

Seed Sci. Res.

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Seed germination is a pivotal period of plant growth and development. This process can be divided into four major stages, swelling absorption, seed coat dehiscence, radicle emergence and radicle elongation. Cupressus gigantea, a tree native to Tibet, China, is characterized by its resistance to stresses such as cold, and drought, and has a high economic and ecological value. Nevertheless, given its unique geographic location, its seeds are difficult to germinate. Therefore, it is crucial to explore the mechanisms involved in seed germination in this species to improve the germination efficiency of its seeds, thereby protecting this high-quality resource. Here, our findings indicate that seed germination was enhanced when exposed to a 6-h/8-h light/dark photoperiod, coupled with a temperature of 20°C. Furthermore, the application of exogenous GA3 (1 mg/ml, about 2.9 mM) stimulated the germination of C. gigantea seeds. Subsequently, proteomics was used to detect changes in protein expression during the four stages of seed germination. We identified 34 differentially expressed proteins (DEPs), including 13 at the radicle pre-emergence stage, and 17 at the radicle elongation stage. These DEPs were classified into eight functional groups, cytoskeletal proteins, energy metabolism, membrane transport, stress response, molecular chaperones, amino acid metabolism, antioxidant system and ABA signalling pathway. Most of them were found to be closely associated with amino acid metabolism. Combined, these findings indicate that, along with temperature and light, exogenous GA3 can increase the germination efficiency of C. gigantea seeds. Our study also offers insights into the changes in protein expression patterns in C. gigantea seeds during germination.

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Section: Discussionmentioning

confidence: 99%

Proteomic characterization of the different stages of seed germination in Cupressus gigantea

Li,

Zhang,

Lei

et al. 2024

Seed Sci. Res.

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“…Since the symbiosome represents the main organelle within these non-vacuolated infected plant cells and the SM osmotic water permeability is higher than normal membrane bilayer diffusion rates, the symbiosome can contribute to the regulation of the cell volume during nitrogen-xing symbiosis (Routray, et al 2015). Besides its osmoregulatory function in infected plant cells, the SM controls the movement of metabolites between the two symbiotic partners, including the transfer of ammonia from the bacteroid to the plant cytoplasm.…”

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confidence: 99%

The nodule-specific multifunctional channel NOD26 of Medicago truncatula is crucial for nitrogen-fixing symbiosis

Frare¹,

Gómez²,

López-Fernández³

et al. 2021

Preprint

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The multifunctional channel NOD26, identified and extensively studied (both biochemically and biophysically) in soybean, is a major protein component of the symbiosome membrane. The water and ammonia transport activities of NOD26 are thought to be important for nodule development, osmotic balance, and ammonia efflux from the symbiosome. However, the widely accepted relevance of NOD26 in nitrogen-fixing symbiosis has never been explored in planta. Recently, we have reported the emergence of NOD26 in the nitrogen-fixing clade of angiosperms via tandem duplication. Here, we characterized the two copies of NOD26 from Medicago truncatula (Medtr8g087710 and Medtr8g087720) in their transport abilities, and at gene expression and genetic levels. Similar to their homologous soybean gene, MtNOD26 genes encode water and ammonia transport activities in heterologous expression systems. By using multiple transcriptional studies (RT-qPCR, transcriptional fusion and RNA-Seq analyses), we found that the expression of MtNOD26 copies is restricted to the nodule and gradually increases from the bacteria-free meristematic region to the nitrogen-fixation zone. Under nitrogen-limiting soil conditions, the homozygous insertional mutant lines of these two MtNOD26 genes had the same aberrant nodulation phenotype and chlorosis. Similar to uninoculated wild-type plants, inoculated mutants were unable to grow in minimal medium without a nitrogen source. Collectively, our findings suggest functional equivalence between MtNOD26 copies and underline a crucial role of NOD26 in symbiotic nitrogen fixation.

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The Nodulin 26 Intrinsic Protein Subfamily

Roberts

Routray

2017

Signaling and Communication in Plants

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Nodulin Intrinsic Proteins: Facilitators of Water and Ammonia Transport across the Symbiosome Membrane

Cited by 6 publications

References 49 publications

Proteomic characterization of the different stages of seed germination in Cupressus gigantea

Proteomic characterization of the different stages of seed germination in Cupressus gigantea

The nodule-specific multifunctional channel NOD26 of Medicago truncatula is crucial for nitrogen-fixing symbiosis

The Nodulin 26 Intrinsic Protein Subfamily

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