Noise analysis and reduction in solid-state nanopores

Tabard‐Cossa, Vincent; Trivedi, Dhruti; Wiggin, Matthew; Jetha, Nahid N.; Marziali, Andre

doi:10.1088/0957-4484/18/30/305505

Cited by 271 publications

(364 citation statements)

References 52 publications

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“…4À7 To suppress noise and further improve SNR, we suggest curing polydimethylsiloxane (PDMS) on the nanopore support chip while leaving the MoS 2 nanopore exposed. 32 …”

Section: Resultsmentioning

confidence: 99%

Atomically Thin Molybdenum Disulfide Nanopores with High Sensitivity for DNA Translocation

et al. 2014

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Atomically thin nanopore membranes are considered to be a promising approach to achieve single base resolution with the ultimate aim of rapid and cheap DNA sequencing. Molybdenum disulfide (MoS2) is newly emerging as a material complementary to graphene due to its semiconductive nature and other interesting physical properties that can enable a wide range of potential sensing and nanoelectronics applications. Here, we demonstrate that monolayer or few-layer thick exfoliated MoS2 with subnanometer thickness can be transferred and suspended on a predesigned location on the 20 nm thick SiN x membranes. Nanopores in MoS2 are further sculpted with variable sizes using a transmission electron microscope (TEM) to drill through suspended portions of the MoS2 membrane. Various types of double-stranded (ds) DNA with different lengths and conformations are translocated through such a novel architecture, showing improved sensitivity (signal-to-noise ratio >10) compared to the conventional silicon nitride (SiN x ) nanopores with tens of nanometers thickness. Unlike graphene nanopores, no special surface treatment is needed to avoid hydrophobic interaction between DNA and the surface. Our results imply that MoS2 membranes with nanopore can complement graphene nanopore membranes and offer potentially better performance in transverse detection.

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“…4À7 To suppress noise and further improve SNR, we suggest curing polydimethylsiloxane (PDMS) on the nanopore support chip while leaving the MoS 2 nanopore exposed. 32 …”

Section: Resultsmentioning

confidence: 99%

Atomically Thin Molybdenum Disulfide Nanopores with High Sensitivity for DNA Translocation

et al. 2014

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“…The overall design, minus the electrodes for clarity, is presented in Figure 1 [108] Alternatively, the nanopore system can be modeled as a simple circuit, as is represented in Figure 1.3. This allows for the utilization of electronics-based calculations to determine relevant information, such as the size of the nanopore.…”

Section: Typical Solid-state Nanopore Setupmentioning

confidence: 99%

Interfacing solid‐state nanopores with gel media to slow DNA translocations

et al. 2015

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We demonstrate the ability to slow DNA translocations through solid‐state nanopores by interfacing the trans side of the membrane with gel media. In this work, we focus on two reptation regimes: when the DNA molecule is flexible on the length scale of a gel pore, and when the DNA behaves as persistent segments in tight gel pores. The first regime is investigated using agarose gels, which produce a very wide distribution of translocation times for 5 kbp dsDNA fragments, spanning over three orders of magnitude. The second regime is attained with polyacrylamide gels, which can maintain a tight spread and produce a shift in the distribution of the translocation times by an order of magnitude for 100 bp dsDNA fragments, if intermolecular crowding on the trans side is avoided. While previous approaches have proven successful at slowing DNA passage, they have generally been detrimental to the S/N, capture rate, or experimental simplicity. These results establish that by controlling the regime of DNA movement exiting a nanopore interfaced with a gel medium, it is possible to address the issue of rapid biomolecule translocations through nanopores—presently one of the largest hurdles facing nanopore‐based analysis—without affecting the signal quality or capture efficiency.

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“…We used a polytetrafluoroethylene cell to mount the nanopore chip between two liquid reservoirs, as described in [12]. Custommade silicone gaskets sandwich the chip to ensure a gigaohm seal.…”

Section: Protocols and Instrumentationmentioning

confidence: 99%

Precise control of the size and noise of solid-state nanopores using high electric fields

et al. 2012

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We present a methodology for preparing silicon nitride nanopores that provides in situ control of size with sub-nanometer precision while simultaneously reducing electrical noise by up to three orders of magnitude through the cyclic application of high electric fields in an aqueous environment. Over 90% of nanopores treated with this technique display desirable noise characteristics and readily exhibit translocation of double-stranded DNA molecules. Furthermore, previously used nanopores with degraded electrical properties can be rejuvenated and used for further single-molecule experiments.

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Noise analysis and reduction in solid-state nanopores

Cited by 271 publications

References 52 publications

Atomically Thin Molybdenum Disulfide Nanopores with High Sensitivity for DNA Translocation

Atomically Thin Molybdenum Disulfide Nanopores with High Sensitivity for DNA Translocation

Interfacing solid‐state nanopores with gel media to slow DNA translocations

Precise control of the size and noise of solid-state nanopores using high electric fields

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