Field-effect transistor (FET) biosensors based on two-dimensional
(2D) materials have drawn significant attention due to their outstanding
sensitivity. However, the Boltzmann distribution of electrons imposes
a physical limit on the subthreshold swing (SS), and a 2D-material
biosensor with sub-60 mV/dec SS has not been realized, which hinders
further increase of the sensitivity of 2D-material FET biosensors.
Here, we report tunnel FETs (TFETs) based on a SnSe2/WSe2 heterostructure and observe the tunneling effect of a 2D
material in aqueous solution for the first time with an ultralow SS
of 29 mV/dec. A bilayer dielectric (Al2O3/HfO2) and graphene contacts, which significantly reduce the leakage
current in solution and contact resistance, respectively, are crucial
to the realization of the tunneling effect in solution. Then, we propose
a novel biosensing method by using tunneling current as the sensing
signal. The TFETs show an extremely high pH sensitivity of 895/pH
due to ultralow SS, surpassing the sensitivity of FET biosensors based
on a single 2D material (WSe2) by 8-fold. Specific detection
of glucose is realized, and the biosensors show a superb sensitivity
(3158 A/A for 5 mM), wide sensing range (from 10–9 to 10–3 M), low detection limit (10–9 M), and rapid response rate (11 s). The sensors also exhibit the
ability of monitoring glucose in complex biofluid (sweat). This work
provides a platform for ultrasensitive biosensing. The discovery of
the tunneling effect of 2D materials in aqueous solution may stimulate
further fundamental research and potential applications.