Non and Epigenetic Mechanisms in Regulation of Adaptive Thermogenesis in Skeletal Muscle

Sahu, Bijayashree; Pani, Sunil; Swalsingh, Gourabamani; Bal, Naresh C.

doi:10.3389/fendo.2019.00517

Cited by 9 publications

(8 citation statements)

References 68 publications

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“…In the case of the offspring at postnatal day 245, an increase in irisin but not in Pgc-1 α was observed, which could indicate that the regulation of thermogenesis in skeletal muscle is mediated by another pathway. 40 Sarcolipin plays a role in metabolism and muscle thermogenesis through Ca 2þ -ATPase of the endoplasmic reticulum (SERCA), 41 and in an animal model of obesity induced by a HFD, sarcolipin/ SERCA expression is decreased. However, irisin treatment increased the expression of both genes.…”

Section: Discussionmentioning

confidence: 99%

(-)-epicatechin treatment did not modify the thermogenic pathway in the gastrocnemius muscle of male rat offspring obeses by programming

Tejeda,

De los Santos,

Coral-Vázquez

et al. 2024

J Dev Orig Health Dis

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The aim of this study was to analyse the expression of genes related to the regulation of energy metabolism in skeletal muscle tissue by comparing male offspring in two age groups [at 110 and 245 postnatal days (pnd)] from a mother with obesity induced by a high-fat diet and (-)-epicatechin (Epi) administration. Four groups of six male offspring from different litters were randomly selected for the control groups [C and offspring of mothers with maternal obesity (MO)] or Epi intervention groups. We evaluated the effect of Epi on gastrocnemius tissue by analysing the mRNA and protein expression levels of Fndc5/irisin, Pgc-1α, Ucp3, and Sln. Epi significantly increased the Pgc-1α protein in the MO group of offspring at 110 pnd (p < 0.036, MO vs. MO+Epi), while at 245 pnd, Epi increased Fndc5/irisin mRNA expression in the MO+Epi group versus the MO group (p = 0.006). No differences were detected in Fndc5/irisin, Ucp3 or Sln mRNA or protein levels (including Pgc-1α mRNA) in the offspring at 110 pnd or in Pgc-1α, Ucp3, or Sln mRNA or protein levels (including Fndc5/irisin protein) at 245 pnd among the experimental groups. In conclusion, (-)-epicatechin treatment increased Fndc5/irisin mRNA expression and Pgc-α protein levels in the gastrocnemius muscle of offspring at postnatal days 110 and 245. Furthermore, it is suggested that the flavonoid effect in a model of obesity and its impact on thermogenesis in skeletal muscle are regulated by a different pathway than Fndc5/irisin.

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Section: Discussionmentioning

confidence: 99%

(-)-epicatechin treatment did not modify the thermogenic pathway in the gastrocnemius muscle of male rat offspring obeses by programming

Tejeda,

De los Santos,

Coral-Vázquez

et al. 2024

J Dev Orig Health Dis

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“…TAAR1 also stimulates cAMP-dependent calcium flux, leading to CaMKII-dependent activation of MEK/ERK signaling. Given that augmented Ca 2+ -handling is associated with skeletal muscle thermogenesis 28 , it is possible that T1AM could also induce thermogenesis in the heart via enhanced Ca 2+ -handling by the activation of PKA or MAPK signaling. However, in the present study, we did not see any significant effects of PKA inhibitor on the actions of T1AM (Supplementary Figs.…”

Section: Discussionmentioning

confidence: 99%

Substantial impact of 3-iodothyronamine (T1AM) on the regulations of fluorescent thermoprobe-measured cellular temperature and natriuretic peptide expression in cardiomyocytes

Nagoshi

Kimura

et al. 2022

Sci Rep

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There is growing interest in 3-iodothyronamine (T1AM), an active thyroid hormone metabolite, that induces negative inotropic and chronotropic actions in the heart and exerts systemic hypothermic action. We explored the direct impact of T1AM on cardiomyocytes with a focus on the regulation of the intracellular temperature and natriuretic peptide (NP) expression. A thermoprobe was successfully introduced into neonatal rat cardiomyocytes, and the temperature-dependent changes in the fluorescence intensity ratio were measured using a fluorescence microscope. After one-hour incubation with T1AM, the degree of change in the fluorescence intensity ratio was significantly lower in T1AM-treated cardiomyocytes than in equivalent solvent-treated controls (P < 0.01), indicating the direct hypothermic action of T1AM on cardiomyocytes. Furthermore, T1AM treatment upregulated B-type NP (BNP) gene expression comparable to treatment with endothelin-1 or phenylephrine. Of note, ERK phosphorylation was markedly increased after T1AM treatment, and inhibition of ERK phosphorylation by an MEK inhibitor completely cancelled both T1AM-induced decrease in thermoprobe-measured temperature and the increase in BNP expression. In summary, T1AM decreases fluorescent thermoprobe-measured temperatures (estimated intracellular temperatures) and increases BNP expression in cardiomyocytes by activating the MEK/ERK pathway. The present findings provide new insight into the direct myocardial cellular actions of T1AM in patients with severe heart failure.

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“…We found two CpG sites, in the gene CLK2 and near the gene TOR4 , that were associated with SaO2. CDC like kinase 2 or CLK2 suppresses PPARGC1A transcriptional activity on gluconeogenic genes ( Sahu et al, 2019 ) and thus downregulates hepatic gluconeogenesis and glucose output. We found CLK2 methylation to be positively associated with SaO 2 , suggesting that CLK2 expression is potentially decreased in hypoxic conditions, given methylation is linked to gene repression.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide DNA Methylation Changes Associated With High-Altitude Acclimatization During an Everest Base Camp Trek

et al. 2021

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The individual physiological response to high-altitude hypoxia involves both genetic and non-genetic factors, including epigenetic modifications. Epigenetic changes in hypoxia factor pathway (HIF) genes are associated with high-altitude acclimatization. However, genome-wide epigenetic changes that are associated with short-term hypoxia exposure remain largely unknown. We collected a series of DNA samples from 15 participants of European ancestry trekking to Everest Base Camp to identify DNA methylation changes associated with incremental altitude ascent. We determined genome-wide DNA methylation levels using the Illumina MethylationEPIC chip comparing two altitudes: baseline 1,400 m (day 0) and elevation 4,240 m (day 7). The results of our epigenome-wide association study revealed 2,873 significant differentially methylated positions (DMPs) and 361 significant differentially methylated regions (DMRs), including significant positions and regions in hypoxia inducible factor (HIF) and the renin–angiotensin system (RAS) pathways. Our pathway enrichment analysis identified 95 significant pathways including regulation of glycolytic process (GO:0006110), regulation of hematopoietic stem cell differentiation (GO:1902036), and regulation of angiogenesis (GO:0045765). Lastly, we identified an association between the ACE gene insertion/deletion (I/D) polymorphism and oxygen saturation, as well as average ACE methylation. These findings shed light on the genes and pathways experiencing the most epigenetic change associated with short-term exposure to hypoxia.

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Non and Epigenetic Mechanisms in Regulation of Adaptive Thermogenesis in Skeletal Muscle

Cited by 9 publications

References 68 publications

(-)-epicatechin treatment did not modify the thermogenic pathway in the gastrocnemius muscle of male rat offspring obeses by programming

(-)-epicatechin treatment did not modify the thermogenic pathway in the gastrocnemius muscle of male rat offspring obeses by programming

Substantial impact of 3-iodothyronamine (T1AM) on the regulations of fluorescent thermoprobe-measured cellular temperature and natriuretic peptide expression in cardiomyocytes

Genome-Wide DNA Methylation Changes Associated With High-Altitude Acclimatization During an Everest Base Camp Trek

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