Non-aqueous encapsulation of excipient-stabilized spray-freeze dried BSA into poly(lactide-co-glycolide) microspheres results in release of native protein

Carrasquillo, Karen G.; Stanley, Ann M; Aponte-Carro, Juan C; Jesús, Patricia De; Costantino, Henry R.; Bosques, Carlos J.; Griebenow, Kai

doi:10.1016/s0168-3659(01)00430-8

Cited by 166 publications

(82 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Thus, water-soluble proteins dispersed in the organic solvents should experience denaturation and aggregation during the electrospinning. This subsequently causes irregular dispersion of proteins in the protein microspheres and the overall encapsulation efficiency can be considerably low (43,44). However, we herein proposed the electrospinning of chitosan solution and encapsulated BSA in the aqueous phase for electrospinning.…”

Section: Resultsmentioning

confidence: 98%

Electrospun Chitosan Microspheres for Complete Encapsulation of Anionic Proteins: Controlling Particle Size and Encapsulation Efficiency

et al. 2013

View full text Add to dashboard Cite

Abstract. Electrospinning was employed to fabricate chitosan microspheres by a single-step encapsulation of proteins without organic solvents. Chitosan in acetic acid was electrospun toward a grounded sodium carbonate solution at various electric potential and feeding rates. Electrospun microspheres became insoluble and solidified in the sodium carbonate solution by neutralization of chitosan acetate. When the freeze-dried microspheres were examined by scanning electron microscopy, the small particle size was obtained at higher voltages. This is explained by the chitosan droplet size at the electrospinning needle was clearly controllable by the electric potential. The recovery yield of chitosan microspheres was dependent on the concentration of chitosan solution due to the viscosity is the major factor affecting formation of chitosan droplet during curling of the electrospinning jets. For protein encapsulation, fluorescently labeled bovine serum albumin (BSA) was codissolved with chitosan in the solution and electrospun. At higher concentration of sodium carbonate solution and longer solidification time in the solution, the encapsulation efficiency of the protein was confirmed to be significantly high. The high encapsulation efficiency was achievable by instant solidification of microspheres and electrostatic interactions between chitosan and BSA. Release profiles of BSA from the microspheres showed that the protein release was faster in acidic solution due to dissolution of chitosan. Reversed-phase chromatography of the released fractions confirmed that exposure of BSA to acidic solution during the electrospinning did not result in structural changes of the encapsulated protein.

show abstract

Section: Resultsmentioning

confidence: 98%

Electrospun Chitosan Microspheres for Complete Encapsulation of Anionic Proteins: Controlling Particle Size and Encapsulation Efficiency

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Hence, the 'active' and 'total' release profiles may be compared and the integrity of the released protein may be assessed. In the same way, the three-dimensional conformation may be analyzed by Size Exclusion Chromatography-HPLC (SEC-HPLC) (Wang et al, 2004), Sodium Dodecyl Sulfate-Poly Acrylamide Gel Electrophoresis (SDS-PAGE) (Woo et al, 2001), Circular Dichroism (CD) (Kwon et al, 2004) and Fourier Transform Infrared Spectroscopy (FTIR) (Carrasquillo et al, 2001).…”

Section: Influence Of the Sampling Methodsmentioning

confidence: 99%

“…The lag phase was reduced and a more continuous release than with PLGA microparticles was observed. It is important to notice that the microsphere diameter was increased by the presence of the poloxamer in the s/o/o procedure (Carrasquillo et al, 2001).…”

Section: Preparation Of Porous Microspheresmentioning

confidence: 99%

How to achieve sustained and complete protein release from PLGA-based microparticles?

Giteau

Venier-Julienne

Aubert-Pouëssel

et al. 2008

International Journal of Pharmaceutics

234

184

View full text Add to dashboard Cite

One of the most challenging tasks in the delivery of therapeutic proteins from PLGAbased microparticles is the sustained and complete release of the protein in its native form.The mechanisms responsible for incomplete protein release from these devices are numerous and complex; the beneficial effect of different formulations has often been evaluated in vitro.Strategies employed for overcoming protein destabilization during the release step are reviewed in this paper. Proteins have been protected in the deleterious environment by adding stabilizers to the formulation, or by modifying the protein or the polymer. Alternatively, some strategies have aimed at avoiding the formation of the destabilizing environment. As experimental conditions may influence the results from in vitro release studies, we initially report precautions to avoid adverse effects.

show abstract

“…These issues may result in aggregation of some delicate proteins and low protein encapsulation efficiency. To avoid protein hydration in the microencapsulation process, a solid-in-oil-in-oil process involving an oil continuous phase was used (84). However, choices of the oil are limited to a few PLG solution-immiscible ones such as silicone oil, which need large amount hydrocarbon to clean up in postmicroencapsulation process.…”

Section: Stabilizing Proteins Prior To Microencapsulation Involving Omentioning

confidence: 99%

Polymer-Based Sustained-Release Dosage Forms for Protein Drugs, Challenges, and Recent Advances

Jin

2008

AAPS PharmSciTech

112

View full text Add to dashboard Cite

Abstract. While the concept of using polymer-based sustained-release delivery systems to maintain therapeutic concentration of protein drugs for extended periods of time has been well accepted for decades, there has not been a single product in this category successfully commercialized to date despite clinical and market demands. To achieve successful systems, technical difficulties ranging from protein denaturing during formulation process and the course of prolonged in vivo release, burst release, and incomplete release, to low encapsulation efficiency and formulation complexity have to be simultaneously resolved. Based on this updated understanding, formulation strategies attempting to address these aspects comprehensively were reported in recent years. This review article (with 134 citations) aims to summarize recent studies addressing the issues above, especially those targeting practical industrial solutions. Formulation strategies representative of three areas, microsphere technology using degradable hydrophobic polymers, microspheres made of water soluble polymers, and hydrophilic in vivo gelling systems will be selected and introduced. To better understand the observations and conclusions from different studies for different systems and proteins, physicochemical basis of the technical challenges and the pros and cons of the corresponding formulation methods will be discussed.

show abstract

Non-aqueous encapsulation of excipient-stabilized spray-freeze dried BSA into poly(lactide-co-glycolide) microspheres results in release of native protein

Cited by 166 publications

References 46 publications

Electrospun Chitosan Microspheres for Complete Encapsulation of Anionic Proteins: Controlling Particle Size and Encapsulation Efficiency

Electrospun Chitosan Microspheres for Complete Encapsulation of Anionic Proteins: Controlling Particle Size and Encapsulation Efficiency

How to achieve sustained and complete protein release from PLGA-based microparticles?

Polymer-Based Sustained-Release Dosage Forms for Protein Drugs, Challenges, and Recent Advances

Contact Info

Product

Resources

About