Non-autofluorescence Detection of H5N1 Virus Using Photochemical Aptamer Sensors Based on Persistent Luminescent Nanoparticles

Abstract

Fluorescence sensing is limited in practical applications owing to multiple autofluorescent substances in complex biological samples such as serum. In this paper, the luminescence decay effect of persistent luminescent nanoparticles (PLNPs) was used to avoid the interference of autofluorescence in complex biological samples, and a non-autofluorescence molecularly imprinted polymer aptamer sensor (MIP-aptasensor) was designed to detect H5N1 virus. The proposed MIP-aptasensor consists of a magnetic MIP and aptamer-functionalized persistent luminescent nanoparticle Zn2GeO4:Mn2+-H5N1 aptamer (ZGO-H5N1 Apt). Upon simultaneous recognition of H5N1 virus, strong persistent luminescent signal changes were produced. Using the unique luminescent characteristics of PLNPs and the high selectivity of imprinted polymers and aptamers, the designed MIP-aptasensor effectively eliminates the autofluorescence background interference of serum samples and realizes the non-autofluorescence detection of H5N1 virus with high sensitivity (a limit of detection of 0.0128 HAU mL–1, 1.16 fM) and selectivity (the imprinting factor for the target H5N1 virus was 6.72). This tool provides a strategy for the design of sensors and their application in complex biological samples.

Programmable Nanostructures Based on Framework-DNA for Applications in Biosensing

“…Recently, association with the latest techniques and other materials has been another trend in this area. A non-self-fluorescent molecularly imprinted polymeric aptasensor (MIP-aptasensor) was designed to detect the H5N1 virus [ 147 ]. Aptamer-functionalized persistent luminescent nanoparticle Zn 2 GeO 4 :Mn 2+ -H5N1 coupled with a magnetic MIP can produce an intense, constant luminescent signal after identifying the H5N1 virus, which has the highly selective and sensitive ability.…”

Near-Infrared Rechargeable Persistent Luminescence Nanoparticles for Biomedical Implants In Vivo Noninvasive Bioimaging

“…Persistent luminescence nanoparticles (PLNPs), also known as long-lasting luminescence or afterglow particles, can continuously emit luminescence for hours or even days after being excited. This special feature allows detection and imaging without external illumination, which can effectively eliminate the interference of autofluorescence on imaging signals. − Therefore, PLNPs exhibit great potential in biological applications ranging from biosensing/bioimaging to theranostics. − However, current research on PLNPs mainly focuses on cancer tissue-targeted biosensing/imaging, , in vivo drug tracking, , and imaging-guided photodynamic therapy, − and few studies in the field of biomedical implants have been reported.…”

Distance-Regulated Photoelectrochemical Sensor “Signal-On” and “Signal-Off” Transitions for the Multiplexed Detection of Viruses Exposed in the Aquatic Environment

“…Water-borne viruses pose a serious threat to public health and safety. , Among them, pathogens such as Norovirus and Rotavirus are well-known causative factors that can lead to nonbacterial gastroenteritis in humans. , The World Health Organization has estimated that millions of people worldwide die every year due to infections caused by Norovirus or Rotavirus. , However, due to the low recognition sensitivity of biological antibodies, traditional immune antigen analysis methods were difficult to meet the detection requirements for multiple types and low loads of exposed viruses in the aqueous environment. , Similarly, polymerase chain reaction (PCR)-based nucleic acid detection methods were equally difficult to satisfy the demand for high-throughput detection of multiplex exposed virus due to their low detection throughput. , Although multiplex PCR techniques have been developed for the simultaneous detection of multiplex nucleic acid sequences, the competition and interference between primer pairs make them highly susceptible to cross-interference when dealing with complex environmental media. , In addition, the high technical requirements of PCR methods, and the complex and time-consuming amplification process make it difficult to meet the demand for immediate detection in the field of environmental analysis . Therefore, there are severe barriers to the application of traditional antigen analysis and nucleic acid detection methods for the detection of viruses exposed to the aqueous environment.…”

“…W ater-borne viruses pose a serious threat to public health and safety. 1,2 Among them, pathogens such as Norovirus and Rotavirus are well-known causative factors that can lead to nonbacterial gastroenteritis in humans. 3,4 The World Health Organization has estimated that millions of people worldwide die every year due to infections caused by Norovirus or Rotavirus.…”