Non-bacterial diarrhoea in children in the Philippines

Paje-Villar, E.; Co, Baughn; Caradang, E. H.; Raymundo, A. D.; Lagamayo, Evelina; Lavadia, E.; Rigor, E.

doi:10.1080/00034983.1994.11812835

Cited by 13 publications

(3 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ward, unpublished data, 1996), and G serotyped and P genotyped by standard methods [Taniguchi et al, 1987;Gentsch et al, 1992]. Strains from Thailand [Hendricks et al, 1995], the Philippines [Paje-Villar et al, 1994], and Uganda (C.A. Hart, unpublished data, 1996) were collected during epidemiologic studies of pediatric gastroenteritis.…”

Section: Rotavirus Strainsmentioning

confidence: 99%

Sequence analysis of NSP4 gene of human rotavirus allows classification into two main genetic groups

et al. 1997

View full text Add to dashboard Cite

The rotavirus nonstructural glycoprotein NSP4 may represent the first identified viral enterotoxin. We have sequenced reverse transcription-polymerase chain reaction (RT-PCR)-generated fragments of 16 NSP4 genes of human rotavirus (HRV) strains from six different countries, representing seven different G and P type combinations. Based on the amount of sequence divergence between these and 11 previously sequenced NSP4 genes of human and animal rotaviruses, three distinct genetic groups could be recognized. Most strains within a group were closely related to each other at the nucleotide (nt) and amino acid (aa) levels (usually <10% divergence) but more distantly related (maximum 30.0% nt divergence and 24.7% aa divergence) to members of the other groups. Intergroup variation occurred in two highly variable regions of NSP4 (aa 16-34 and aa 131-148). The NSP4 "toxic peptide" (aa 114-135) exhibited aa variation at its carboxy terminus both within and between genetic groups. The largest group (genetic group II) contained HRV strains of subgroup II specificity (including genotypes P[8]G1, P[8]G3, P[6]G3, and P[8]G5 and serotype P8[11]G9), and the smaller group (genetic group I) contained HRV strains of subgroup I specificity (genotype P[4]G2). The NSP4 sequence of the rhesus rotavirus vaccine strain was distinct from all other strains and formed the third group (genetic group III). The NSP4 genes of animal rotaviruses UK, NCDV, and SA11 (genetic group I) and YM (genetic group II) and two possible human-animal rotavirus reassortant strains, Brazilian P[8]G5 and Indian P[11]G9 (genetic group II), could also be classified into one of these groups, suggesting a close evolutionary relationship between human and animal NSP4 genes. These results will facilitate studies of the host immune response to NSP4, which may be relevant to future HRV vaccine design.

show abstract

Section: Rotavirus Strainsmentioning

confidence: 99%

Sequence analysis of NSP4 gene of human rotavirus allows classification into two main genetic groups

et al. 1997

View full text Add to dashboard Cite

show abstract

“…Infection rates in young children and infants living in developing countries may have been underestimated in previous studies that sought to identify viral particles in stool specimens by electron microscopy [Singh et al, 1989;Timenetsky et al, 1993;Paje-Villar et al, 1994]. The development of a recombinant antigen enzymelinked immunosorbent assay (ELISA) has enabled studies of the sero-epidemiology of pediatric NLV infection in developing countries, and has led to an accumulation of evidence that these viruses commonly infect young children and infants.…”

Section: Introductionmentioning

confidence: 97%

Genomic diversity of ?Norwalk like viruses? (NLVs): Pediatric infections in a Brazilian shantytown

et al. 1999

View full text Add to dashboard Cite

"Norwalk-like viruses" (NLVs) are a common cause of epidemic gastroenteritis in adults and children in developed countries. However, little is known about the role of NLVs in endemic pediatric gastroenteritis in developing countries. We sequenced Genogroup I and II NLV reverse transcription-polymerase chain reaction (RT-PCR) products from an 81-nucleotide region of the viral RNA polymerase gene to examine the molecular epidemiology of NLV infection in children younger than 5 years of age in Forteleza, Ceará, Brazil. NLV-positive PCR products were obtained from stool specimens collected over a 16-month period (1990-1991) from diarrhea cases and controls in a cohort of 120 children in an urban shantytown and from a study in the same city of hospitalized children with persistent diarrhea. Eight unique strains were detected in 15 specimens from 10 cohort children and in two hospital specimens. Nucleotide identity between the strains (5 Genogroup I, 3 Genogroup II) ranged from 63% to 88%. We designated these strains BraV1-8, for Brazil virus 1-8. The degree of genomic diversity of NLV strains we identified in this cohort during a short time period suggests multiple foci of infection within the community. Furthermore, sequence analysis of strains from two children with multiple symptomatic NLV infections indicates that infection with one strain was not protective against subsequent infection with a different strain in the same genogroup. These findings have implications for vaccine development and the prevention of pediatric gastroenteritis in developing countries.

show abstract

“…In fact, no reports on the incidence of the disease in the country were published since 1994 (Menorca et al 1994;Paje-Villar et al 1994). Moreover, it is not routinely diagnosed in the country's medical institutions.…”

mentioning

confidence: 97%

Serological detection of cryptosporidiosis among Filipino cancer patients

2005

View full text Add to dashboard Cite

Information on cryptosporidiosis in the Philippines is limited. To date, the disease is not routinely diagnosed in the country's medical institutions. To this end, a total of 53 Filipino cancer patients were surveyed for cryptosporidiosis using an indirect immunofluorescent antibody test. Fifteen patients (28.3%) were found to be positive for antibodies against Cryptosporidium. This study contributes to a better understanding of the incidence of cryptosporidiosis in the country.

show abstract

Non-bacterial diarrhoea in children in the Philippines

Cited by 13 publications

References 14 publications

Sequence analysis of NSP4 gene of human rotavirus allows classification into two main genetic groups

Sequence analysis of NSP4 gene of human rotavirus allows classification into two main genetic groups

Genomic diversity of ?Norwalk like viruses? (NLVs): Pediatric infections in a Brazilian shantytown

Serological detection of cryptosporidiosis among Filipino cancer patients

Contact Info

Product

Resources

About