1995
DOI: 10.1002/j.1460-2075.1995.tb00237.x
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Non-bilayer lipids are required for efficient protein transport across the plasma membrane of Escherichia coli.

Abstract: The construction of a mutant Escherichia coli strain which cannot synthesize phosphatidylethanolamine provides a tool to study the involvement of non‐bilayer lipids in membrane function. This strain produces phosphatidylglycerol and cardiolipin (CL) as major membrane constituents and requires millimolar concentrations of divalent cations for growth. In this strain, the lipid phase behaviour is tightly regulated by adjustment of the level of CL which favours a nonbilayer organization in the presence of specific… Show more

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Cited by 141 publications
(109 citation statements)
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“…Thus, one possibility is that changes in the shape of MGD molecules compensate for disruptive changes in the shape of PG molecules induced by the fab1 mutation, for example by altering the packing relationships between the thylakoid lipids and membrane proteins of the photosynthetic complexes (Gounaris and Barber, 1983;Simidjiev et al, 2000). Changes in the shape of lipid molecules is known to be important for a number of membrane functions including protein trafficking and membrane fusion (Rietveld et al, 1995;Chanturiya et al, 1997;Bruce, 1998). Certainly, the decreased unsaturation of lipids in fab1 fad5-2 plants relative to fab1 means that it is reasonable to consider molecular shape rather than membrane fluidity or phase changes as the basis of the suppressor phenotype.…”
Section: Discussionmentioning
confidence: 99%
“…Thus, one possibility is that changes in the shape of MGD molecules compensate for disruptive changes in the shape of PG molecules induced by the fab1 mutation, for example by altering the packing relationships between the thylakoid lipids and membrane proteins of the photosynthetic complexes (Gounaris and Barber, 1983;Simidjiev et al, 2000). Changes in the shape of lipid molecules is known to be important for a number of membrane functions including protein trafficking and membrane fusion (Rietveld et al, 1995;Chanturiya et al, 1997;Bruce, 1998). Certainly, the decreased unsaturation of lipids in fab1 fad5-2 plants relative to fab1 means that it is reasonable to consider molecular shape rather than membrane fluidity or phase changes as the basis of the suppressor phenotype.…”
Section: Discussionmentioning
confidence: 99%
“…How are integral membrane proteins integrated into the E. coli membrane and does this involve the lateral opening of the translocase to release the membrane segments into the lipid phase. : Other issues to be resolved concern the molecular mechanism of proofreading, how preproteins are released at the trans-side of the translocase, the role of the low-affinity lipid bound SecA in translocation, and the mechanism by which non-bilayer lipids stimulate translocation [49]. Finally; an unresolved aspect concerns the catalytic mechanism by which the Ap drives translocation.…”
Section: Discussionmentioning
confidence: 99%
“…PE adopts a non-bilayer hexagonal II phase conformation (31 ) (32,35). These cations stimulate the bilayer to non-bilayer transition of CL, which comprises 44% of the total phospholipid in this strain (36).…”
mentioning
confidence: 99%
“…It therefore appears that the polymorphic behavior of these lipids is essential for growth, a role normally fulfilled by PE. The requirement for non-bilayer lipids for protein translocation appears less strict than for anionic lipids (35), but the mechanism by which these lipids act on protein translocation is unknown. Non-bilayer lipids only marginally affect the SecA lipid ATPase activity (37) and appear not essential for the functional reconstitution of protein translocation using octyl glucoside (OG)-purified SecYEG complex (11).…”
mentioning
confidence: 99%