Non‐Cell‐Adhesive Substrates for Printing of Arrayed Biomaterials

Appel, Eric A.; Larson, Benjamin L.; Luly, Kathryn M.; Kim, Jinseong D.; Langer, Robert

doi:10.1002/adhm.201400594

Cited by 7 publications

(6 citation statements)

References 37 publications

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“…Stiffness and thermal responses are fundamental properties of hydrogels important for their chemical, biological and medical applications [34] . In addition, the non‐cell adhesive property is required for implanted materials, substrates for cell arrayed biodevices and biocompatible materials [35–37] . This study demonstrates the effectiveness and impact of inserting an alkylene chain at the center of an amphiphilic peptide with an identical amino acid sequence to tune the mechanical and biological properties of peptidic materials.…”

Section: Discussionmentioning

confidence: 95%

Hydrogel‐Stiffening and Non‐Cell Adhesive Properties of Amphiphilic Peptides with Central Alkylene Chains

Yaguchi

Hiramatsu

Ishida

et al. 2021

Chemistry A European J

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Amphiphilic peptides bearing terminal alkyl tails form supramolecular nanofibers that are increasingly used as biomaterials with multiple functionalities. Insertion of alkylene chains in peptides can be designed as another type of amphiphilic peptide, yet the influence of the internal alkylene chains on self-assembly and biological properties remains poorly defined. Unlike the terminal alkyl tails, the internal alkylene chains can affect not only the hydrophobicity but also the flexibility and packing of the peptides. Herein, we demonstrate the supramolecular and biological effects of the central alkylene chain length inserted in a peptide. Insertion of the alkylene chain at the center of the peptide allowed for strengthened β-sheet hydrogen bonds and modulation of the packing order, and consequently the amphiphilic peptide bearing C2 alkylene chain formed a hydrogel with the highest stiffness. Interestingly, the amphiphilic peptides bearing internal alkylene chains longer than C2 showed a diminished cell-adhesive property. This study offers a novel molecular design to tune mechanical and biological properties of peptide materials.

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Section: Discussionmentioning

confidence: 95%

Hydrogel‐Stiffening and Non‐Cell Adhesive Properties of Amphiphilic Peptides with Central Alkylene Chains

Yaguchi

Hiramatsu

Ishida

et al. 2021

Chemistry A European J

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“…The epoxy groups are intended to form covalent linkages with the pHEMA, although they may just act as a compatible surface, and dip-coating is readily achieved by immersion and removal of the slide into a 4% (w/v) pHEMA solution in ethanol, preferably under automation . Agarose dip-coating on aminoalkylsilanated slides is also a widely used strategy, showing negligible background cell attachment and retaining stability throughout the polymer printing and UV sterilization processes. , Another reported polymer is PEG, synthesized by incorporating a small proportion of functionalized PEG molecules into the underlying silane formulation, it generates a substrate that can be used for the covalent binding of peptides, natural polymers, hydrogels or synthetic polymer biomaterials. , Preparations of the nonionic surfactant Pluronic have also been used, typically in combination with biomolecule-based microarrays, as a commercially available and cell-compatible formulation that effectively repels cell binding …”

Section: Microarray Strategies Applied In Biomaterials Screening and ...mentioning

confidence: 99%

High-Throughput Methods in the Discovery and Study of Biomaterials and Materiobiology

et al. 2021

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The complex interaction of cells with biomaterials (i.e., materiobiology) plays an increasingly pivotal role in the development of novel implants, biomedical devices, and tissue engineering scaffolds to treat diseases, aid in the restoration of bodily functions, construct healthy tissues, or regenerate diseased ones. However, the conventional approaches are incapable of screening the huge amount of potential material parameter combinations to identify the optimal cell responses and involve a combination of serendipity and many series of trial-and-error experiments. For advanced tissue engineering and regenerative medicine, highly efficient and complex bioanalysis platforms are expected to explore the complex interaction of cells with biomaterials using combinatorial approaches that offer desired complex microenvironments during healing, development, and homeostasis. In this review, we first introduce materiobiology and its high-throughput screening (HTS). Then we present an in-depth of the recent progress of 2D/3D HTS platforms (i.e., gradient and microarray) in the principle, preparation, screening for materiobiology, and combination with other advanced technologies. The Compendium for Biomaterial Transcriptomics and high content imaging, computational simulations, and their translation toward commercial and clinical uses are highlighted. In the final section, current challenges and future perspectives are discussed. High-throughput experimentation within the field of materiobiology enables the elucidation of the relationships between biomaterial properties and biological behavior and thereby serves as a potential tool for accelerating the development of high-performance biomaterials.

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“…[ 6 ] Alternatively, the array could be directly retained on the non‐cytoadherent substrate by physical interactions. [ 7 ] In both cases, the substrate on which the biomaterial array has to be fabricated should be compatible with multiple factors, for example, fabrication procedures, non‐toxic, different biomaterials, etc. The properties of common substrates used for fabrication of different microarrays are listed in Table 1 .…”

Section: Fabrication Of High‐throughput Biomaterials Platformsmentioning

confidence: 99%

“…The coating with PEG (molecular weight 500 Da) exhibited hydrophilic properties as compared to tissue culture polystyrene plate as measured by water contact angle and negligible cell adhesion. [ 7 ] In another example, 100 nm thick poly(oligo(ethyleneglycol) methacrylate) polymer brush was used as a non‐fouling substrate onto which antibody microarray was constructed by non‐covalent absorption. The array so developed exhibited a long shelf life with no compromise in activity.…”

Section: Fabrication Of High‐throughput Biomaterials Platformsmentioning

confidence: 99%

Recent Advances in Biomaterial‐Based High‐Throughput Platforms

Sarkar

Kumar

2020

Biotechnology Journal

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High‐throughput systems allow screening and analysis of large number of samples simultaneously under same conditions. Over recent years, high‐throughput systems have found applications in fields other than drug discovery like bioprocess industries, pollutant detection, material microarrays, etc. With the introduction of materials in such HT platforms, the screening system has been enabled for solid phases apart from conventional solution phase. The use of biomaterials has further facilitated cell‐based assays in such platforms. Here, the authors have focused on the recent developments in biomaterial‐based platforms including the fabricationusing contact and non‐contact methods and utilization of such platforms for discovery of novel biomaterials exploiting interaction of biological entities with surface and bulk properties. Finally, the authors have elaborated on the application of the biomaterial‐based high‐throughput platforms in tissue engineering and regenerative medicine, cancer and stem cell studies. The studies show encouraging applications of biomaterial microarrays. However, success in clinical applicability still seems to be a far off task majorly due to absence of robust characterization and analysis techniques. Extensive focus is required for developing personalized medicine, analytical tools and storage/shelf‐life of cell laden microarrays.

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Non‐Cell‐Adhesive Substrates for Printing of Arrayed Biomaterials

Cited by 7 publications

References 37 publications

Hydrogel‐Stiffening and Non‐Cell Adhesive Properties of Amphiphilic Peptides with Central Alkylene Chains

Hydrogel‐Stiffening and Non‐Cell Adhesive Properties of Amphiphilic Peptides with Central Alkylene Chains

High-Throughput Methods in the Discovery and Study of Biomaterials and Materiobiology

Recent Advances in Biomaterial‐Based High‐Throughput Platforms

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