Non-classical effects of prolactin on the innate immune response of bovine mammary epithelial cells: Implications during Staphylococcus aureus internalization

Medina-Estrada, Ivan; Alva-Murillo, Nayeli; López‐Meza, Joel E.; Ochoa‐Zarzosa, Alejandra

doi:10.1016/j.micpath.2015.08.018

Cited by 13 publications

(38 citation statements)

References 46 publications

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“…As we reported previously, S. aureus decreases the α5β1 integrin MA and induces the TLR2 MA in bMECs, but does not alter the CD36 MA (Alva-Murillo et al, 2015; Medina-Estrada et al, 2015). The α5β1 integrin MA was increased (~2.5-fold) by 0.25 mM NaO treatment, and this induction did not change after bacterial stimulus (Figure 2A).…”

Section: Resultssupporting

confidence: 73%

“…In addition, it is known that α5β1 integrin, TLR2, and CD36 play an important role in the S. aureus internalization into bMECs (Alva-Murillo et al, 2015; Medina-Estrada et al, 2015). However, the participation of these receptors in the NaO mediated-internalization process is unknown.…”

Section: Resultsmentioning

confidence: 99%

“…Epithelial cells (ECs) participate in this defense through pattern recognition of conserved molecules associated with microorganisms (PAMPs) by means of various families of germ-line-encoded pattern-recognition receptors, including the Toll-like receptors (TLRs) (Bulek et al, 2010). The production of innate immune effectors (enzymes, AP, cytokines, chemokines) is due to the stimulation of ECs through diverse receptors, which in turn leads to the activation of MAPK family proteins (p38, JNK and ERK1/2) and transcription factors (e.g., NF-κB, AP-1, E2F-1, EGR, FAST-1) (Akira et al, 2006; Chiu et al, 2009; Alva-Murillo et al, 2015; Medina-Estrada et al, 2015). …”

Section: Introductionmentioning

confidence: 99%

“…This chronicity is related to the ability of S. aureus to internalize into professional and NPPCs, such as bMECs (Garzoni and Kelley, 2011; Fraunholz and Sinha, 2012; Scali et al, 2015). In NPPCs, the internalization process depends primarily on the host α5β1 integrin (Hauck et al, 2012; Medina-Estrada et al, 2015). However, other host cell receptors are involved in this process (Alva-Murillo et al, 2014a).…”

Section: Introductionmentioning

confidence: 99%

“…However, other host cell receptors are involved in this process (Alva-Murillo et al, 2014a). In this sense, the blockage of TLR2 in bMECs, the most relevant receptor for S. aureus recognition, with neutralizing antibodies decreases the number of internalized bacteria (Alva-Murillo et al, 2015; Medina-Estrada et al, 2015). On the other hand, CD36, a scavenger receptor and a fatty acid translocase, plays a role in S. aureus recognition and internalization mainly in professional phagocytic cells by interacting with TLR2 (Hoebe et al, 2005; Stuart et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

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Sodium Octanoate Modulates the Innate Immune Response of Bovine Mammary Epithelial Cells through the TLR2/P38/JNK/ERK1/2 Pathway: Implications during Staphylococcus aureus Internalization

Alva-Murillo

Ochoa‐Zarzosa

López‐Meza

2017

Front. Cell. Infect. Microbiol.

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Bovine mammary epithelial cells (bMECs) contribute to mammary gland defense against invading pathogens, such as Staphylococcus aureus (intracellular facultative), which is recognized by TLR2. In a previous report, we showed that sodium octanoate [NaO, a medium chain fatty acid (C8)] induces (0.25 mM) or inhibits (1 mM) S. aureus internalization into bMECs and differentially regulates the innate immune response (IIR). However, the molecular mechanisms have not been described, which was the aim of this study. The results showed that α5β1 integrin membrane abundance (MA) was increased in 0.25 mM NaO-treated cells, but TLR2 or CD36 MA was not modified. When these receptors were blocked individually, 0.25 mM NaO-increased S. aureus internalization was notably reduced. Interestingly, in this condition, the IIR of the bMECs was impaired because MAPK (p38, JNK, and ERK1/2) phosphorylation and the activation of transcription factors related to these pathways were decreased. In addition, the 1 mM NaO treatment induced TLR2 MA, but neither the integrin nor CD36 MA was modified. The reduction in S. aureus internalization induced by 1 mM NaO was increased further when TLR2 was blocked. In addition, the phosphorylation levels of the MAPKs increased, and 13 transcriptional factors related to the IIR were slightly activated (CBF, CDP, c-Myb, AP-1, Ets-1/Pea-3, FAST-1, GAS/ISRE, AP-2, NFAT-1, OCT-1, RAR/DR-5, RXR/DR-1, and Stat-3). Moreover, the 1 mM NaO treatment up-regulated gene expression of IL-8 and RANTES and secretion of IL-1β. Notably, when 1 mM NaO-treated bMECs were challenged with S. aureus, the gene expression of IL-8 and IL-10 increased, while IL-1β secretion was reduced. In conclusion, our results showed that α5β1 integrin, TLR2 and CD36 are involved in 0.25 mM NaO-increased S. aureus internalization in bMECs. In addition, 1 mM NaO activates bMECs via the TLR2 signaling pathways (p38, JNK, and ERK1/2), which improves IIR before S. aureus invasion. Additionally, NaO (1 mM) might exert anti-inflammatory effects after bacterial internalization.

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Section: Resultssupporting

confidence: 73%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Sodium Octanoate Modulates the Innate Immune Response of Bovine Mammary Epithelial Cells through the TLR2/P38/JNK/ERK1/2 Pathway: Implications during Staphylococcus aureus Internalization

Alva-Murillo

Ochoa‐Zarzosa

López‐Meza

2017

Front. Cell. Infect. Microbiol.

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Puerarin Exerts an Antiinflammatory Effect by Inhibiting NF-kB and MAPK Activation inStaphylococcus aureus-Induced Mastitis

Zhao

Jiang

et al. 2016

Phytother. Res.

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Mastitis is defined as the inflammation of the mammary gland. There is generally no effective treatment for mastitis in animals. Puerarin, extracted from Radix puerariae, has been proven to possess many biological activities. The present study aims to reveal the potential mechanism that is responsible for the antiinflammatory action of puerarin in Staphylococcus aureus (S. aureus)-induced mastitis in mice. Histopathological changes showed that puerarin ameliorated the inflammatory injury induced by S. aureus. Quantitative real-time polymerase chain reaction and ELISA analysis indicated that puerarin not only suppressed the production of pro-inflammatory cytokines such as TNF-α, IL-1β, and IL-6 but also promoted the secretion of IL-10. Toll-like receptor 2 (TLR2) is important in the immune defense against S. aureus infection. Research in molecular biology has shown that the expression of TLR2 was inhibited with administration of puerarin. Further studies were performed on NF-kB and mitogen-activated protein kinase signaling pathways using western blot. The results demonstrated that puerarin suppressed phosphorylated IkBα, p65, p38, extracellular signal-regulated kinase 1and 2 (ERK), and c-Jun N-terminal kinase (JNK) in a dose-dependent manner. All of the results suggested that puerarin may be a potential therapy for treating mastitis. Copyright © 2016 John Wiley & Sons, Ltd.

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Minthostachys verticillata essential oil modulates cytokine synthesis and Staphylococcus aureus internalization in MAC-T cells at least through TLR4/MyD88/NFkB pathway

Arsaute,

Reinoso,

Cecchini

et al. 2024

Vet Res Commun

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Non-classical effects of prolactin on the innate immune response of bovine mammary epithelial cells: Implications during Staphylococcus aureus internalization

Cited by 13 publications

References 46 publications

Sodium Octanoate Modulates the Innate Immune Response of Bovine Mammary Epithelial Cells through the TLR2/P38/JNK/ERK1/2 Pathway: Implications during Staphylococcus aureus Internalization

Sodium Octanoate Modulates the Innate Immune Response of Bovine Mammary Epithelial Cells through the TLR2/P38/JNK/ERK1/2 Pathway: Implications during Staphylococcus aureus Internalization

Puerarin Exerts an Antiinflammatory Effect by Inhibiting NF-kB and MAPK Activation inStaphylococcus aureus-Induced Mastitis

Minthostachys verticillata essential oil modulates cytokine synthesis and Staphylococcus aureus internalization in MAC-T cells at least through TLR4/MyD88/NFkB pathway

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