Non‐coding RNAs as the bridge between epigenetic mechanisms, lineages and domains of life

Sela, Mor; Kloog, Yoel; Rechavi, Oded

doi:10.1113/jphysiol.2014.273045

Cited by 6 publications

(5 citation statements)

References 28 publications

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“…Non-coding RiboNucleic Acid (small or large nc-RNA) are involved in epigenetic regulation directly silencing chromatin at specific loci by base pairing to nascent transcripts (Cech and Steitz, 2014). Among these ncRNAs, miRNAs are proposed to play a significant role in epigenetic regulation opening new avenues for therapeutic purposes (Sethupathy, 2016) or for assaying exogenous non-coding RNA acquisition on the physiology of host and putative consequences across generations (Sela et al, 2014; Junien et al, 2016). Milk contains a high amount of miRNA which has been proposed for transfer between mother and child for immune regulation (Kosaka et al, 2010), for priming the immune system of the lactating infant when from plant origin (Lukasik et al, 2017) or for trans-species effect on adult consumers through dairy products (Baier et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Oral Delivery of miRNA With Lipidic Aminoglycoside Derivatives in the Breastfed Rat

Beuzelin¹,

Pitard

Kaeffer³

2019

Front. Physiol.

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Context Specific targeting of endogenous miRNAs which are involved in epigenetics, may help understanding homeostasis with therapeutic benefits. We use new biologically inspired vehicles consisting of lipoaminoglycosides to deliver in vivo mir-320-3p, a known human breast milk exosomal miRNA, or its antagomiR. Materials and Methods Four lipoaminoglycosides were screened for cytotoxicity and their biophysical properties. 1-h breast-restricted rats received single-oral treatment of either the lipoaminoglycoside Dioleyl-Succinyl Paromomycin (DOSP) complexed with miRNA or antagomiR, or of control medium at the light on (ZeitGeber Time: ZT-0H) or off (ZT-12H). Glycemia, triglycerides, cholesterol, free-fatty acid were assayed at 0, 4, 8, and 12 h post-treatment. In the stomach, small intestine, liver, plasma, adipose tissue, plexus choroid, and cortex, relevant miRNA with precursors and mRNA (polr3d, hspb6, c-myc, stat1, clock, bmal1, per1, npas2, sirt1-6, and cyclinD1) were quantified by q-PCR. Expression of POLR3D and HSPB6 proteins were analyzed in stomach and liver by Western blot. Immunoprecipitations with anti-AGO1 and 2 were performed on nuclear and cytoplasmic fractions of gastric cells along with detection of miRNA-320-3p in nucleoli. Chromatin ImmunoPrecipitation with anti-Trimethyl-histone-3-Lys-4 and Lys-27 detecting the polr3d promoter and miR-320-3p, were performed for all groups. Results Selected DOSP (diameter: 80–200 nm) did not alter gastric extracellular vesicle secretion a few hours after intake. The miR-320-3p was mainly found in gastric or small intestinal cells, reaching the blood and liver in low amount. We have found significant up-regulation of polr3d mRNA (ANOVA, p < 0.0001) at ZT-20H for the miR-320-3p-supplemented group and a higher expression of POLR3D for antagomiR group (ANOVA, p < 0.05). We had a low accumulation of miR-320-3p at ZT-20H in nucleoli, without stat1 evolution. Delivering a high amount of miRNA or antagomiR disrupts RNA-Induced Silencing Complexes in cytoplasm triggering some transfer of extracellular molecules into nuclei with alteration of immune complexes on the polr3d promoter (with a higher amount found in the K4 histone-3-me3 immune complexes at ZT-20H). Conclusion Extracellular miRNAs embedded in DOSP have a rapid impact on RNAi and on nuclear chromatin complexes depending on the daily rhythm. An integrative view of the impact of extracellular miRNA on physiology will improve assaying epigenetic manipulations following nutritional stress.

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Section: Introductionmentioning

confidence: 99%

Oral Delivery of miRNA With Lipidic Aminoglycoside Derivatives in the Breastfed Rat

Beuzelin¹,

Pitard

Kaeffer³

2019

Front. Physiol.

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“…Noble et al, 2014), and most particularly work focused on trans-generational inheritance mediated by those mechanisms (e.g. Danchin et al, 2011;Dias and Ressler, 2014;Gluckman et al, 2007;Klironomos et al, 2013;Nelson et al, 2012;Rechavi et al, 2011;Sela et al, 2014), has created the need to either extend or replace the Modern (neo-Darwinist) Synthesis (Beurton et al, 2008;Gissis and Jablonka, 2011;Noble et al, 2014;Pigliucci and Müller, 2010). This paper explains why replacement rather than extension is called for.…”

Section: Introductionmentioning

confidence: 99%

Evolution beyond neo-Darwinism: a new conceptual framework

Noble

2015

Journal of Experimental Biology

115

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There was an error published in J. Exp. Biol. 218, 7-13.The quotation from a Nature 2010 editorial on page 10 of the article does not appear in the final published version of the editorial. The correct quotation is given below.'But for all the intellectual ferment of the past decade, has human health truly benefited from the sequencing of the human genome? A startlingly honest response can be found on pages 674 and 676, where the leaders of the public and private efforts, Francis Collins and Craig Venter, both say "not much".'We apologise for any inconvenience this may have caused. 1273

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“…SYNTHÈSE REVUES disciplinaire, qui seule permettra d'imaginer de nouvelles approches, de découvrir des mécanismes encore insoupçonnés et de développer des stratégies de prévention adaptées, cohérentes et efficaces chez les sujets à risque. Quant à la transmission aux générations suivantes de susceptibilité à des maladies chroniques ou d'une rési-lience, par le biais de mécanisme(s) épigénétique(s) qui sont encore mal étayés, elle n'est pas encore démontrée très clairement chez l'humain, mais les preuves s'accumulent chez le rongeur, les nématodes, la drosophile et surtout les plantes [54][55][56][57][58]. Jusqu'à présent un nombre important de revues portent sur les effets inter-ou transgénérationnels [15][16][17]59].…”

Section: Le Principe Des Deux éVénements : Effets Ou Réponses ?unclassified

Le nouveau paradigme de l’origine développementale de la santé et des maladies (DOHaD)

et al. 2016

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Non‐coding RNAs as the bridge between epigenetic mechanisms, lineages and domains of life

Cited by 6 publications

References 28 publications

Oral Delivery of miRNA With Lipidic Aminoglycoside Derivatives in the Breastfed Rat

Oral Delivery of miRNA With Lipidic Aminoglycoside Derivatives in the Breastfed Rat

Evolution beyond neo-Darwinism: a new conceptual framework

Le nouveau paradigme de l’origine développementale de la santé et des maladies (DOHaD)

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