Non-coding RNAs change their expression profile after Retinoid induced differentiation of the promyelocytic cell line NB4

Abstract

BackgroundThe importance of non-coding RNAs (ncRNAs) as fine regulators of eukaryotic gene expression has emerged by several studies focusing on microRNAs (miRNAs). miRNAs represent a newly discovered family of non coding-RNAs. They are thought to be crucial players of human hematopoiesis and related tumorigenesis and to represent a potential tool to detect the early stages of cancer. More recently, the expression regulation of numerous long ncRNAs has been linked to cell growth, differentiation and cancer although the molecular mechanism of their function is still unknown.NB4 cells are promyelocytic cells that can be induced to differentiation upon retinoic acid (ATRA) treatment and represent a feasible model to study changes of non coding RNAs expression between cancer cells and their terminally differentiated counterpart.Findingswe screened, by microarray analysis, the expression of 243 miRNAs and 492 human genes transcribing for putative long ncRNAs different from miRNAs in NB4 cells before and after ATRA induced differentiation. Our data show that 8 miRNAs, and 58 long ncRNAs were deregulated by ATRA induced NB4 differentiation.Conclusionour data suggest that ATRA-induced differentiation lead to deregulation of a large number of the ncRNAs that can play regulatory roles in both tumorigenesis and differentiation.

Massive Effect on LncRNAs in Human Monocytes During Fungal and Bacterial Infections and in Response to Vitamins A and D

“…In addition to micro RNAs (miRNAs) being regulated by atRA1314, several lncRNAs have been reported to be influenced by atRA in neuronal and cancer cells. In rats, atRA is involved in hippocampal functions by up-regulating LINC_RBE15 and is able to restore NEAT1 expression in NB4 leukemia cell differentiation16.…”

MicroRNAs as targets for dietary and pharmacological inhibitors of mutagenesis and carcinogenesis

“…In APL cells, RA upregulated the expression of miR-186, miR-215 and miR-223 , while it downregulated the expression of miR-17, miR-25, miR-193, miR-195 [29]. In another study using the same cells, RA was found to upregulate the expression of miR-15a, miR-15b, miR-16-1, let-7a-3, let-7c, let-7d, miR-107, miR-223, and miR-342 , whereas miR-181b was downregulated [30].…”

The role of vitamins and minerals in modulating the expression of microRNA

“…Furthermore, the recruitment of NF-kB on the let-7a-3/let-7b cluster promoter upon treatment with retinoic acid suggests that this is the mechanism of regulation of miRNA expression in this case (125) . Conversely, authors of a later study of ATRA differentiation in NB4 cells reported that let-7a was down-regulated following ATRA treatment (126) . This apparent contradiction is probably due to the differences in culture time used in the two experiments (48 h (125) , compared with 6 d (126) ).…”

“…Conversely, authors of a later study of ATRA differentiation in NB4 cells reported that let-7a was down-regulated following ATRA treatment (126) . This apparent contradiction is probably due to the differences in culture time used in the two experiments (48 h (125) , compared with 6 d (126) ). This highlights the importance of the temporal specificity of the relationships between nutrients, miRNA expression and physiological consequences and this needs to be considered in furthering these studies.…”