Non-coding RNAs in endothelial cell signalling and hypoxia during cardiac regeneration

Peters, Marijn M C; Sampaio-Pinto, Vasco; Martins, Paula

doi:10.1016/j.bbamcr.2019.07.010

Cited by 11 publications

(5 citation statements)

References 110 publications

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“…In fact, corresponding changes in the transcriptomic signature of the cardiac myocytes, CPC-derived EVs have demonstrated an ability to decrease tissue stiffness and BNP release and exhibited beneficial effects with regard to post-STEMI remodeling (49). Additionally, CPC-derived EVs contain a distinct repertoire of biologically active miRNAs, such as microRNA-373 and microRNA-21, that have strongly yielded anti-fibrotic effects and ameliorated fibrosis in the infarcted area targeting key pro-fibrogenic genes, i.e., TGF-β, GDF-11, and ROCK-2 (51,52). Interestingly, EVs significantly inhibited microRNA-21 degradation and thereby mediate the anti-apoptotic effect in cardiac myocytes and endothelial cells (53).…”

Section: Cardiomyocyte Progenitor Cell-derived Evsmentioning

confidence: 99%

“…There are four epigenetic mechanisms: histone acetylation, histone methylation, DNA hyper-and hypo-methylation, and non-coding RNA regulation. Multiple pre-clinical and clinical studies have shown that non-coding RNAs transferred by EVs are the most important epigenetic regulators of cell differentiation, proliferation, survival, development, regeneration, and neovascularization (52,105,106). Interestingly, some subsets of free cell non-coding RNAs, such as mi-RNAs, are normally derived to the target cells by high-density lipoproteins (107), however, the majority of long non-coding RNAs and short chains of mi-RNAs are enriched and stable in EVs and can be delivered by EVs acting as gene regulators (108).…”

Section: Ev-derived Non-coding Rnas In Cardiac and Vascular Remodelinmentioning

confidence: 99%

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Extracellular Endothelial Cell-Derived Vesicles: Emerging Role in Cardiac and Vascular Remodeling in Heart Failure

Berezin

2020

Front. Cardiovasc. Med.

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Extracellular vesicles play a pivotal role in numerous physiological (immune response, cell-to-cell cooperation, angiogenesis) and pathological (reparation, inflammation, thrombosis/coagulation, atherosclerosis, endothelial dysfunction) processes. The development of heart failure is strongly associated with endothelial dysfunction, microvascular inflammation, alteration in tissue repair, and cardiac and vascular remodeling. It has been postulated that activated endothelial cell-derived vesicles are not just transfer forms of several active molecules (such as regulatory peptides, coagulation factors, growth factors, active molecules, hormones that are embedded onto angiogenesis, tissue reparation, proliferation, and even prevention from ischemia/hypoxia), but are instead involved in direct myocardial and vascular damage due to regulation of epigenetic responses of the tissue. These responses are controlled by several factors, such as micro-RNAs, that are transferred inside extracellular vesicles from mother cells to acceptor cells and are transductors of epigenetic signals. Finally, it is not a uniform opinion whether different phenotypes of heart failure are the result of altered cardiac and vascular reparation due to certain epigenetic responses, which are yielded by co-morbidities, such as diabetes mellitus and obesity. The aim of the review is to summarize knowledge regarding the role of various types of extracellular endothelial cell-derived vesicles in the regulation of cardiac and vascular remodeling in heart failure.

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Section: Cardiomyocyte Progenitor Cell-derived Evsmentioning

confidence: 99%

Section: Ev-derived Non-coding Rnas In Cardiac and Vascular Remodelinmentioning

confidence: 99%

Extracellular Endothelial Cell-Derived Vesicles: Emerging Role in Cardiac and Vascular Remodeling in Heart Failure

Berezin

2020

Front. Cardiovasc. Med.

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“…The indirect pro-angiogenic activity is the transport of miR-210 through exosomes secreted by tumor cells [151,152]. Under hypoxic conditions, miR-210 has the ability to inhibit ephrin-A3 receptor tyrosine kinase expression [153][154][155]. As a result of this interaction, increased secretion of VEGF occurs, which stimulates the formation of new blood vessels [156,157].…”

Section: Exosomesmentioning

confidence: 99%

Important players in carcinogenesis as potential targets in cancer therapy: an update

2020

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The development of cancer is a problem that has accompanied mankind for years. The growing number of cases, emerging drug resistance, and the need to reduce the serious side effects of pharmacotherapy are forcing scientists to better understand the complex mechanisms responsible for the initiation, promotion, and progression of the disease. This paper discusses the modulation of the particular stages of carcinogenesis by selected physiological factors, including: acetylcholine (ACh), peroxisome proliferator-activated receptors (PPAR), fatty acid-binding proteins (FABPs), Bruton's tyrosine kinase (Btk), aquaporins (AQPs), insulin-like growth factor-2 (IGF-2), and exosomes. Understanding their role may contribute to the development of more effective and safer therapies based on new binding sites.

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“…On the other hand, ECs have been found to play important roles in maintaining homeostasis by promoting blood vessel formation and building blood vessel barriers [ 11 ]. Neovascularization is a key process in cardiac regeneration [ 12 ]. According to previous studies on the angiogenesis of ECs, E2F1 inhibits VEGF and PlGF upregulation through p53 dependent and independent mechanisms, respectively, thereby limiting apoptosis and neovascularization of cardiac ECs after MIn [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Single-cell RNA sequencing reveals hub genes of myocardial infarction-associated endothelial cells

Wang,

Dou

2024

BMC Cardiovasc Disord

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Background Myocardial infarction (MI) is a cardiovascular disease that seriously threatens human health. Dysangiogenesis of endothelial cells (ECs) primarily inhibits recovery from MI, but the specific mechanism remains to be further elucidated. Methods In this study, the single-cell RNA-sequencing data from both MI and Sham mice were analyzed by the Seurat Package (3.2.2). The number of ECs in MI and Sham groups were compared by PCA and tSNE algorithm. FindMarkers function of Seurat was used to analyze the DEGs between the MI and Sham groups. Then, the ECs was further clustered into 8 sub-clusters for trajectory analysis. The BEAM was used to analyze the branch point 3 and cluster the results. In addition, the DEGs in the microarray data set of MI and Sham mice were cross-linked, and the cross-linked genes were used to construct PPI networks. The key genes with the highest degree were identified and analyzed for functional enrichment. Finally, this study cultured human umbilical vein endothelial cells (HUVECs), established hypoxia models, and interfered with hub gene expression in cells. The impact of hub genes on the migration and tube formation of hypoxic-induced HUVECs were verified by Wound healing assays and tubule formation experiments. Results The number and proportion of ECs in the MI group were significantly lower than those in the Sham group. Meantime, 225 DEGs were found in ECs between the MI and Sham groups. Through trajectory analysis, EC4 was found to play an important role in MI. Then, by using BEAM to analyze the branch point 3, and clustering the results, a total of 495 genes were found to be highly expressed in cell Fate2 (mainly EC4). In addition, a total of 194 DEGs were identified in Micro array dataset containing both MI and Sham mice. The hub genes (Timp1 and Fn1) with the highest degree were identified. Inhibiting Timp1 and Fn1 expression promoted the migration and tube formation of HUVECs. Conclusions Our data highlighted the non-linear dynamics of ECs in MI, and provided a foothold for analyzing cardiac homeostasis and pro-angiogenesis in MI.

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Non-coding RNAs in endothelial cell signalling and hypoxia during cardiac regeneration

Cited by 11 publications

References 110 publications

Extracellular Endothelial Cell-Derived Vesicles: Emerging Role in Cardiac and Vascular Remodeling in Heart Failure

Extracellular Endothelial Cell-Derived Vesicles: Emerging Role in Cardiac and Vascular Remodeling in Heart Failure

Important players in carcinogenesis as potential targets in cancer therapy: an update

Single-cell RNA sequencing reveals hub genes of myocardial infarction-associated endothelial cells

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