Sacred natural sites are the oldest form of protection for non-human species and landscapes, and remain significant for conservation and development. This paper critically interrogates the role of spiritual worldviews and witchcraft in protected areas. Drawing on research in Mbozi District, Tanzania, we discuss the entanglements between spiritual worldviews, witchcraft, political leadership, religion and non-humans. Adopting assemblage as a conceptual framework offers possibilities to examine the agencies that tangible and intangible forms non-humans and human organisations have in conservation. Employing assemblage concepts including gradients, territorialisation and deterritorialisation, this paper argues for recognition of the complex assemblages between development, conservation and rationality.