Non-contact microdrilling of mouse zona pellucida with an objective-delivered 1.48-μm diode laser

Rink, Klaus; Delacrétaz, G.; Salathé, R. P.; Senn, A.; Nocera, Dorotha; Germond, Marc; Grandi, P. De; Fakan, Stanislav

doi:10.1002/(sici)1096-9101(1996)18:1<52::aid-lsm7>3.0.co;2-q

Cited by 85 publications

(57 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Several murine studies showed that Ca +2 depletion caused remodeling of the cellular cytoskeleton, inevitably affecting compaction [35][36][37][38]. Furthermore, artificial breaching of the zona pellucida by means of the laser system to enable blastomere biopsy may increase local temperature for a few milliseconds and potentially impact subsequent development [39], although Taylor et al had shown that laser pulse does not influence embryo development when the size of the hole is controlled [40][41][42][43]. All these interruptions in culture conditions, including the total longer duration outside the incubator, can cause the observed delay in further development.…”

Section: Discussionmentioning

confidence: 99%

Blastomere biopsy for PGD delays embryo compaction and blastulation: a time-lapse microscopic analysis

Bar-El

Kalma

Malcov

et al. 2016

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose The purpose of the study was to explore the effect of blastomere biopsy for preimplantation genetic diagnosis (PGD) on the embryos' dynamics, further cleavage, development, and implantation. Methods The study group included 366 embryos from all PGD treatments (September 2012 to June 2014) cultured in the EmbryoScope™ time-lapse monitoring system. The control group included all intracytoplasmic sperm injection (ICSI) embryos cultured in EmbryoScope™ until day 5 during the same time period (385 embryos). Time points of key embryonic events were analyzed with an EmbryoViewer™. Results Most (88 %) of the embryos were biopsied at ≥8 cells. These results summarize the further dynamic development of the largest cohort of biopsied embryos and demonstrate that blastomere biopsy of cleavage-stage embryos significantly delayed compaction and blastulation compared to the control non-biopsied embryos. This delay in preimplanation developmental events also affected postimplantation development as observed when the dynamics of non-implanted embryos (known implantation data (KID) negative) were compared to those of implanted embryos (KID positive). Conclusion Analysis of morphokinetic parameters enabled us to explore how blastomere biopsy interferes with the dynamic sequence of developmental events. Our results show that biopsy delays the compaction and the blastulation of the embryos, leading to a decrease in implantation.

show abstract

Section: Discussionmentioning

confidence: 99%

Blastomere biopsy for PGD delays embryo compaction and blastulation: a time-lapse microscopic analysis

Bar-El

Kalma

Malcov

et al. 2016

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

“…Subsequently, the contact approach was used with ultraviolet (UV) wavelength delivered by glass pipette, or infrared (IR) wavelength delivered with a quartz fibre [53]. However, the technical advantages of the non-contact mode and the potential of UV radiation to cause harmful mutagenic effects have led to the general preference for non-contact mode using the IR 1.48 μm (1,480 nm) diode laser [55].…”

Section: Laser-assisted Hatchingmentioning

confidence: 99%

Assisted hatching in assisted reproduction: a state of the art

Hammadeh

Fischer-Hammadeh

Ali

2010

J Assist Reprod Genet

106

View full text Add to dashboard Cite

The World Health Organization estimates that one in six couples experience some delay in conception and an increasing number require treatment by the assisted conception (AC) procedures of in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI).The implantation rate of embryos resulting from in vitro fertilization cycles is generally less than 20%. The exposure of oocytes and embryos to the artificial conditions of in vitro culture may have negative effects on the embryo's ability to undergo normal hatching, resulting in low rates of implantation following IVF and embryo transfer. Human embryos resulting from superovulation develop more slowly in vitro compared to embryos in vivo, manifest a relatively high degree of cytogenetic abnormalities and undergo cellular fragmentation. Artificially disrupting the zona pellucida is known as assisted hatching (AH) and there is some evidence that embryos that have undergone zona manipulation for assisted hatching tend to implant one day earlier than unhatched embryos. A variety of techniques have since been employed to assist embryo hatching, including partial mechanical zona dissection, zona drilling and zona thinning, making use of acid tyrodes, proteinases, piezon vibrator manipulators and lasers. This review will consider the impact of IVF conditions on zona pellucida physiology, zona hardening, different techniques of assisted hatching, who may benefit from assisted hatching and potential hazards.

show abstract

“…The first of the newer generation of lasers to be used in IVF was the 2.9 um pulsed erbium:yttrium-aluminum-garnet laser (Er:YAG) (Feichtinger et al, 1992). This device's use is limited by the need for constant contact with the embryo, as well as limitations due to interactions with the liquid media (Rink et al, 1996). The next development was the holmium:yttrium-scandium-gallium-garnet laser (Ho:YSGG) with 2.1 um emission.…”

Section: History Of Lasers In Ivfmentioning

confidence: 99%