Non-covalent complexes between single-stranded oligodeoxynucleotides and poly(ethylene imine)

Smiljanić, Danijela; Wesdemiotis, Chrys

doi:10.1016/j.ijms.2010.06.035

Cited by 11 publications

(9 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The dissociation energies of polymer ions represent an intrinsic structural property; however, their exact values are difficult to determine because of the numerous competitive and consecutive reactions of such large species and the excess energy needed to cause fragmentation within the short residence time of the ions in the mass spectrometer (kinetic shift) . Information about relative fragmentation energetics can still be gained by energy‐resolved MS 2 experiments . For this, a polymer ion is subjected to CAD at different collision energies and the survival yield (SY), defined as the intensity of the polymer ion divided by the total ion intensity in the MS 2 (CAD) spectrum, is plotted against the corresponding center‐of‐mass collision energy ( E CM ) to construct a SY curve.…”

Section: Tandem Mass Spectrometry (Ms2)mentioning

confidence: 99%

Multidimensional Mass Spectrometry of Synthetic Polymers and Advanced Materials

2017

Self Cite

View full text Add to dashboard Cite

Multidimensional mass spectrometry interfaces a suitable ionization technique and mass analysis (MS) with fragmentation by tandem mass spectrometry (MS ) and an orthogonal online separation method. Separation choices include liquid chromatography (LC) and ion-mobility spectrometry (IMS), in which separation takes place pre-ionization in the solution state or post-ionization in the gas phase, respectively. The MS step provides elemental composition information, while MS exploits differences in the bond stabilities of a polymer, yielding connectivity and sequence information. LC conditions can be tuned to separate by polarity, end-group functionality, or hydrodynamic volume, whereas IMS adds selectivity by macromolecular shape and architecture. This Minireview discusses how selected combinations of the MS, MS , LC, and IMS dimensions can be applied, together with the appropriate ionization method, to determine the constituents, structures, end groups, sequences, and architectures of a wide variety of homo- and copolymeric materials, including multicomponent blends, supramolecular assemblies, novel hybrid materials, and large cross-linked or nonionizable polymers.

show abstract

Section: Tandem Mass Spectrometry (Ms2)mentioning

confidence: 99%

Multidimensional Mass Spectrometry of Synthetic Polymers and Advanced Materials

2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…Die Spaltungsenergie von Polymer‐Ionen ist eine spezifische Struktureigenschaft; ihre genaue Bestimmung ist jedoch schwierig, zum einen wegen der vielen Konkurrenz‐ und Folgereaktionen solcher großer Spezies, zum anderen wegen der Überschussenergie, die benötigt wird, um eine Fragmentierung während der kurzen Verweilzeit der Ionen im Massenspektrometer zu bewirken (kinetische Verschiebung) . Durch energieauflösende MS/MS‐Experimente können jedoch zumindest Informationen über die relative Energetik der Fragmentierung gewonnen werden . Hierzu wird ein Polymer‐Ion der CAD mit unterschiedlichen Kollisionsenergien unterzogen, und der “Überlebensgrad” (SY), definiert als die Intensität des Polymer‐Ions dividiert durch die Gesamtionenintensität im MS/MS(CAD)‐Spektrum, wird gegen die Kollisionsenergie im Schwerpunktsystem ( E CM ) aufgetragen, um eine SY‐Kurve zu erhalten.…”

Section: Tandem‐massenspektrometrie (Ms/ms)unclassified

Mehrdimensionale Massenspektrometrie von synthetischen Polymeren und modernen Materialien

Wesdemiotis

2017

Angewandte Chemie

Self Cite

View full text Add to dashboard Cite

Mehrdimensionale Massenspektrometrie koppelt eine geeignete Ionisationsmethode und Massenanalyse (MS) mit der Fragmentierung mittels Tandem‐Massenspektrometrie (MS/MS) und einem Online‐Verfahren der orthogonalen Trennung. Für die Trennung kommen Flüssigkeitschromatographie (LC) und Ionenmobilitätsspektrometrie (IMS) in Frage, wobei die Trennung vor der Ionisation in der Lösung bzw. nach der Ionisation in der Gasphase erfolgt. Die MS liefert Daten zur Elementzusammensetzung, während die MS/MS Unterschiede der Bindungsstabilitäten eines Polymers nutzt, wodurch Daten zur Konnektivität und Sequenz erhalten werden. Die Bedingungen der LC können so gewählt werden, dass eine Trennung nach Polarität, Endgruppenfunktionalität oder hydrodynamischem Volumen möglich wird, während die IMS zusätzliche Selektivität bezüglich der Makromolekülform und ‐architektur liefert. In diesem Kurzaufsatz wird erörtert, wie ausgewählte Kombinationen der MS‐, MS/MS‐, LC‐ und IMS‐Dimensionen in Verbindung mit der entsprechenden Ionisationsmethode eingesetzt werden können, um die Bestandteile, Endgruppen, Sequenzen und Strukturen eines breiten Spektrums an Homopolymer‐ und Copolymermaterialien zu ermitteln, das Mehrkomponentenmischungen, supramolekulare Aggregate, neuartige Hybridmaterialien und große, vernetzte oder nichtionisierbare Polymere umfasst.

show abstract

“…Recently, IM-MS(/MS) has been successfully used to differentiate defective and ideal structures of low-generation PAMAM [16]; structural information as well as conformation study could be achieved. Otherwise, IM-MS has been an effective technique for the study of DNA structure [17,18] and complex mixtures of [DNA/ligand] complexes such as polyplexes [19,20].…”

Section: Introductionmentioning

confidence: 99%

Investigation of Dendriplexes by Ion Mobility-Mass Spectrometry

et al. 2014

View full text Add to dashboard Cite

Abstract:Highly branched polyamidoamine (PAMAM) dendrimers presenting biological activities have been envisaged as non-viral gene delivery vectors. They are known to associate with nucleic acid (DNA) in non-covalent complexes via electrostatic interactions. Although their transfection efficiency has been proved, PAMAMs present a significant cytotoxicity due to their cationic surface. To overcome such a drawback, different chemical modifications of the PAMAM surface have been reported such as the attachment of hydrophobic residues. In the present work, we studied the complexation of DNA duplexes with different low-generation PAMAM; ammonia-cored G0(N) and G1(N) PAMAM, native or chemically modified with aromatic residues, i.e., phenyl-modified-PAMAM G0(N) and phenylalanine-modified-PAMAM G1(N). To investigate the interactions involved in the PAMAM/DNA complexes, also called dendriplexes, we used electrospray ionization (ESI) coupled to ion mobility spectrometry-mass-spectrometry (IM-MS). ESI is known to allow the study of non-covalent complexes in native conditions while IM-MS is a bidimensional separation technique particularly useful for the characterization of complex mixtures. IM-MS allows the separation of the expected complexes, possible additional non-specific complexes and the free ligands. Tandem mass spectrometry (MS/MS) was also used for the structural characterization. This work highlights the contribution of IM-MS and MS/MS for the study of small dendriplexes. The stoichiometries of the complexes and the

show abstract

Non-covalent complexes between single-stranded oligodeoxynucleotides and poly(ethylene imine)

Cited by 11 publications

References 32 publications

Multidimensional Mass Spectrometry of Synthetic Polymers and Advanced Materials

Multidimensional Mass Spectrometry of Synthetic Polymers and Advanced Materials

Mehrdimensionale Massenspektrometrie von synthetischen Polymeren und modernen Materialien

Investigation of Dendriplexes by Ion Mobility-Mass Spectrometry

Contact Info

Product

Resources

About