Abstract:Yeast surface display represents a commonly used platform suitable for the generation and screening of antibodies as well as the selection of high-producer clones. The methods of yeast display rely on the genetic fusion of recombinant antibodies to an abundant cell wall protein of yeast. Here, the study of proof of concept showed that the conventional strategy of expression of fusion antibodies was replaced by non-covalent binding to antibodies for yeast surface display. The use of cell surface display of an e… Show more
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