Non-destructive assessment of spore germination of VAM fungi and production of pot cultures from single spores

Brundrett, Mark; Juniper, Sato

doi:10.1016/0038-0717(94)00135-n

Cited by 36 publications

(18 citation statements)

References 15 publications

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“…Germinated spores were defined as those with a germ tube extended from the spore for a distance greater than the diameter of the spore (9). Statistical significance of germination outcomes was calculated by using Fisher's exact test (Prism GraphPad 2.01).…”

Section: Methodsmentioning

confidence: 99%

Invasion of Spores of the Arbuscular Mycorrhizal Fungus Gigaspora decipiens by Burkholderia spp

Levy

Chang

Abbott³

et al. 2003

Appl Environ Microbiol

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Burkholderia species are bacterial soil inhabitants that are capable of interacting with a variety of eukaryotes, in some cases occupying intracellular habitats. Pathogenic and nonpathogenic Burkholderia spp., including B. vietnamiensis, B. cepacia, and B. pseudomallei, were grown on germinating spores of the arbuscular mycorrhizal fungus Gigaspora decipiens. Spore lysis assays revealed that all Burkholderia spp. tested were able to colonize the interior of G. decipiens spores. Amplification of specific DNA sequences and transmission electron microscopy confirmed the intracellular presence of B. vietnamiensis. Twelve percent of all spores were invaded by B. vietnamiensis, with an average of 1.5 ؋ 10 6 CFU recovered from individual infected spores. Of those spores inoculated with B. pseudomallei, 7% were invaded, with an average of 5.5 ؋ 10 5 CFU recovered from individual infected spores. Scanning electron and fluorescence microscopy provided insights into the morphology of surfaces of spores and hyphae of G. decipiens and the attachment of bacteria. Burkholderia spp. colonized both hyphae and spores, attaching to surfaces in either an end-on or side-on fashion. Adherence of Burkholderia spp. to eukaryotic surfaces also involved the formation of numerous fibrillar structures.

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Section: Methodsmentioning

confidence: 99%

Invasion of Spores of the Arbuscular Mycorrhizal Fungus Gigaspora decipiens by Burkholderia spp

Levy

Chang

Abbott³

et al. 2003

Appl Environ Microbiol

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“…In the first experiment, spores in soil cores collected from Auscott in 1994 that had been stored dry for 1 yr were compared with spores from freshly collected soil . Spores were extracted from soil, and collected on 100-/im and 50-/im sieves because of the suggestion that large spores are more likely to be viable than small spores (Brundrett & Juniper, 1995). For comparison, spores were also extracted from a fresh pot culture of Glomus mosseae (Nicol.…”

Section: Viability Of Sporesmentioning

confidence: 99%

Survival of propagules of arbuscular mycorrhizal fungi in soils in eastern Australia used to grow cotton

et al. 1997

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SUMMARYSoil-borne spores and hj^phae of arbuscular mycorrhizal fungi are important propagules in cracking clay soils of northern NSW, Australia. In these soils, senescent roots were uncommon. Although c. 4-200 spores g~^ soil were found, less than 6 % established arbuscular mycorrhizas in trap plants, and this percentage declined over 24 months. Using tetrazolium red as a vital stain, 16-21 % of spores from field soils were found to be viable in fresh soil and 6-7 % after 24 months of storage. Using fluorescein diacetate, the length of stained hyphae of c. 0-5 m g~^ soil was shown to be halved over 32 wk. The density of viable propagules of arbuscular mycorrhizal fungi in soil declined over time and was reduced by severe disturbance. The fungi that survived to 12 months included a species thought to form dormant spores, while those initiating infection after 24 months, did not.

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“…Voucher samples determined as G. intraradices growing in the culture were made on 9 August 2002 (W4064), 17 March 2003 (W4344), and 3 March 2004 (W4598). From the 2004 sampling, a single spore was germinated on a filter fragment (Brundrett & Juniper, 1995) and successfully used to establish Att1102‐7 with P. lanceolata . Spores formed in this culture corresponded broadly to the type material of G. intraradices .…”

mentioning

confidence: 99%

‘Glomus intraradices DAOM197198’, a model fungus in arbuscular mycorrhiza research, is not Glomus intraradices

2009

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Summary• Glomus intraradices-like fungi are the most intensely studied arbuscular mycorrhizal (AM) fungi. However, there are several AM fungi named as G. intraradices that may not be conspecific. Therefore, the hypothesis was tested that DAOM197198 and similar AM fungi, such as BEG195, correspond to the type of G. intraradices.• The G. intraradices isotype material, a descendant (INVAM FL208) of the type culture, and a morphologically corresponding AM fungus (MUCL49410) isolated from the type locality were studied and compared with several cultures of DAOM197198 and BEG195.• Phylogenetic analyses of the partial small subunit (SSU), complete internal transcribed spacer (ITS) and partial large subunit (LSU) nuclear rDNA regions revealed two clades, one including G. intraradices FL208 and MUCL49410, the other containing DAOM197198 and BEG195.• The two clades were clearly separated by sequence analyses, despite the high intraspecific and intrasporal ITS region sequence divergence of up to > 23%. We conclude that the AM fungi with the identifiers DAOM197198 and BEG195 are not G. intraradices, but fall in a clade that contains the recently described species G. irregulare.

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Non-destructive assessment of spore germination of VAM fungi and production of pot cultures from single spores

Cited by 36 publications

References 15 publications

Invasion of Spores of the Arbuscular Mycorrhizal Fungus Gigaspora decipiens by Burkholderia spp

Invasion of Spores of the Arbuscular Mycorrhizal Fungus Gigaspora decipiens by Burkholderia spp

Survival of propagules of arbuscular mycorrhizal fungi in soils in eastern Australia used to grow cotton

‘Glomus intraradices DAOM197198’, a model fungus in arbuscular mycorrhiza research, is not Glomus intraradices

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