ObjectivesThe texture of plant‐based meat substitute is closely related to its microstructure. X‐ray microscopy (XRM) is a non‐destructive imaging technology that can provide high‐resolution three‐dimensional (3D) images of the internal structure of objects. XRM can visualize the 3D structure of meat‐like fibrous structure and realize 3D reconstruction of fibrous structure.MethodsThis research method provides a more comprehensive spatial characterization of the plant protein fibrous structure formation process by 3D analysis through several steps of plant‐based meat substitute preparation, freeze‐drying and XRM measurement of the extrudate.Expected OutcomesThe non‐invasive nature of XRM makes it possible to visualize the internal structure without destroying fibrous structure. The research results provide new research methods and theoretical basis for the study of “plant‐based meat substitute” and other foods.