Non-destructive egg breed separation using advanced VOC analytical techniques HSSE-GC-MS, PTR-TOF-MS, and SIFT-MS: Assessment of performance and systems’ complementarity

Corion, Matthias; Portillo-Estrada, Miguel; Santos, Simão; Lammertyn, Jeroen; De Ketelaere, Bart; Hertog, Maarten

doi:10.1016/j.foodres.2023.113802

Cited by 7 publications

(2 citation statements)

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“…The GC–MS was equipped with a 30 μm × 250 μm × 0.25 μm HP-5MS column (Agilent Technologies). The device conditions for the desorption and the subsequent analysis are described in detail in ref .…”

Section: Methodsmentioning

confidence: 99%

“…Volatile organic compounds (VOCs) from avian hatching eggs are known to carry biological information concerning breed origin, , sex, − and fertility. , Particularly, acquiring details about both the sex and fertilization status would result in significant improvements in commercial incubation procedures. In specific sectors where only one sex is preferred for commercial purposes, in ovo sex determination would offer distinct advantages .…”

Section: Introductionmentioning

confidence: 99%

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Noninvasive Assessment of Chicken Egg Fertility during Incubation Using HSSE–GC–MS VOC Profiling

Corion,

Monteiro Belo dos Santos,

Everaert

et al. 2024

J. Agric. Food Chem.

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Volatile organic compounds (VOCs) carry crucial information about chicken egg fertility. Assessing the fertility before incubation holds immense potential for poultry industry efficiency. Our study used headspace sorptive extraction−gas chromatography−mass spectrometry to analyze egg VOCs before and during the initial 12 incubation days. A total of 162 VOCs were identified. Hexanal was significantly higher in unfertilized eggs, whereas compounds such as propan-2-ol, propan-2-one, and carboxylic acids were higher in fertilized eggs. Furthermore, the obtained multiple logistic regression model outperformed the partial least-squares-discriminant analysis (PLS-DA) model, demonstrating lower complexity and superior performance. Fertile eggs were accurately identified in the validation set in 68−75% of the cases during the initial 4 days, to 85 and 100% on days 6 and 8. Finally, hierarchical cluster analysis in fertilized eggs revealed the clustering of VOCs of the same chemical class, indicative of their shared biochemical origin. This suggests a promising direction for future research aimed at understanding the biological information embedded in VOCs and their relationship to biochemical processes during embryo development.

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Section: Methodsmentioning

confidence: 99%