Non-diarrheagenic and diarrheagenic E. coli carrying supplementary virulence genes (SVG) are associated with diarrhea in children from Mexico

Angulo-Zamudio, Uriel A.; Gutiérrez-Jiménez, Javier; Monroy-Higuera, Luis; Flores-Villaseñor, Héctor; León-Sicairos, Nidia; Velázquez-Román, Jorge; Vidal, Jorge E.; Tapia-Pastrana, Gabriela; Canizalez-Roman, Adrián

doi:10.1016/j.micpath.2021.104994

Cited by 10 publications

(16 citation statements)

References 59 publications

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“…E. coli and Klebsiella spp. were resistant to multiple antibiotics, as observed in other similar studies in Mexico [32,35,36]. A study conducted in Egypt also reported a high resistance to antibiotics in E. coli and in K. pneumoniae isolated from patients with CRC, where more than 71% of those strains were resistant to 10 antibiotics.…”

Section: Discussionsupporting

confidence: 75%

“…Notably, cdt was the most prevalent cyclomodulin in this study; despite the relationship between cdt and DNA damage, this toxin has also been related to serious cases of diarrhea in Mexican subjects due to E. coli [30,31]. However, we found an association of cdt with non-DECs, but recent work indicates that non-DECs with supplementary virulence genes have high levels of virulence, and are capable of producing diarrhea [32]. The high prevalence of diarrheas from E. coli in Mexico could explain the amount of cdt in our study population [33].…”

Section: Discussioncontrasting

confidence: 69%

“…More evidence is needed to establish a possible relationship between antibiotic resistance and cyclomodulins; nevertheless, we cannot rule out the resistance of the present microbiome in subjects from other countries. In the case of Mexico, bacteria isolated from humans, water, or foods have a high level of resistance to antimicrobials [32,35,36].…”

Section: Discussionmentioning

confidence: 99%

“…To obtain genomic DNA, 10 colonies were taken per sample, and inoculated into LB broth media incubated at 37 • C for 18 h. Each bacterial pellet was collected and suspended in 200 µL of molecular biology grade water, and then the boiling method was applied [32]. The DNA was stored at −20 • C for future experiments.…”

Section: Preparation Of Template Dnamentioning

confidence: 99%

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Prevalence of Cyclomodulin-Positive E. coli and Klebsiella spp. Strains in Mexican Patients with Colon Diseases and Antimicrobial Resistance

et al. 2021

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Colon diseases, such as colorectal cancer (CRC), are multifactor diseases that affect more than one million people per year; recently, the microbiota has been associated with an etiologic factor, specifically bacterial cyclomodulin positivity (CM+). Unfortunately, there are no studies from Mexico that detail the presence of bacterial CM+ in patients with colon diseases. We therefore performed a comprehensive study to investigate the associations and prevalence of cyclomodulin-positive Diarrheagenic E. coli (DEC), non-DEC, and Klebsiella spp. strains isolated from Mexican subjects with colon diseases. In this work, we analyzed 43 biopsies, 87 different bacteria were isolated, and E. coli was the most frequently noted, followed by Klebsiella spp., and Enterococcus spp. E. coli, non-DEC, and EPEC belonging to phylogroup B2 were the most prevalent. More than 80% of E. coli and Klebsiella were CM+. pks, cdt, cnf, and cif were identified. cdt was associated with non-DEC, cif and its combinations with EPEC, as well as cdt and psk with Klebsiella. Lastly, all the CM+ bacteria were resistant to at least one antibiotic (34% were MDR, and 48% XDR). In conclusion, the high prevalence of bacterial CM+ in colon disease patients suggests that these bacteria play an important role in the genesis of these diseases.

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Section: Discussionsupporting

confidence: 75%

Section: Discussioncontrasting

confidence: 69%

Section: Discussionmentioning

confidence: 99%

Section: Preparation Of Template Dnamentioning

confidence: 99%

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Prevalence of Cyclomodulin-Positive E. coli and Klebsiella spp. Strains in Mexican Patients with Colon Diseases and Antimicrobial Resistance

et al. 2021

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“…The presence of DEC pathotypes forming biofilms can cause a serious threat because it can cause difficulties in medical treatments [27,28]. DEC pathotypes, especially ETEC, either make biofilms or attach to already existing biofilms.…”

Section: Introductionmentioning

confidence: 99%

Detection of Virulence Genes and Biofilm Forming Capacity of Diarrheagenic E. coli Isolated from Different Water Sources

et al. 2021

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Diarrheagenic Escherichia coli (DEC) are associated with frequent incidences of waterborne infections and pose health risk to individuals who contact water for domestic or recreational uses. Detection of DEC pathotypes in drinking water can be used as an indicator of fecal contamination. This study aimed to investigate the occurrence of DEC pathotypes and their capacity to form biofilms in drinking water samples collected from different water sources. In this study, PCR analysis was used to determine the occurrence of four clinically significant virulence genes of diarrheagenic E. coli, eaeA (Enteropathogenic E. coli), stx1, stx2 (Enterohemorrhagic E. coli) and sth (Enterotoxigenic E. coli), in drinking water samples (n = 35) by using specific primers and conditions. PCR amplicons were visualized by using agarose gel electrophoresis. A total of 12/35 (34%) samples were detected as positive for at least one of the four DEC virulence genes and 11/12 (91%) E. coli isolates harbored virulence gene while 1/12 (8%) E. coli isolates harbored none. The eaeA and sth genes were the most detected genes (75%), while stx1 and stx2 genes were least detected genes (66%). Biofilm assay confirmed that ETEC pathotypes can cause damage in enteric walls by attaching and effacing to persist diarrheal conditions. This study indicated that drinking water of different sources is contaminated with potential DEC pathotypes and it can be a source of diarrheal diseases. The amplification of four virulence genes associated with DEC pathotypes (EPEC, EHEC and ETEC) in drinking water demonstrates that potentially virulent DEC pathotypes are distributed in water sources and may be a cause of health concern. There is, therefore, an urgent need to monitor DEC pathotypes in drinking water.

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Potentially Pathogenic Bacteria in Nesting Olive Ridley Turtles in Northwestern Mexico

Jorge,

Uriel,

Nidia

et al. 2023

EcoHealth

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Non-diarrheagenic and diarrheagenic E. coli carrying supplementary virulence genes (SVG) are associated with diarrhea in children from Mexico

Cited by 10 publications

References 59 publications

Prevalence of Cyclomodulin-Positive E. coli and Klebsiella spp. Strains in Mexican Patients with Colon Diseases and Antimicrobial Resistance

Prevalence of Cyclomodulin-Positive E. coli and Klebsiella spp. Strains in Mexican Patients with Colon Diseases and Antimicrobial Resistance

Detection of Virulence Genes and Biofilm Forming Capacity of Diarrheagenic E. coli Isolated from Different Water Sources

Potentially Pathogenic Bacteria in Nesting Olive Ridley Turtles in Northwestern Mexico

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