Non-dietary therapies for celiac disease

Yoosuf, Shakira; Therrien, Amélie; Leffler, Daniel A.

doi:10.1016/b978-0-12-821571-5.00011-8

Cited by 2 publications

(2 citation statements)

References 181 publications

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“…E40 is a secreted protein obtained from the soil bacterium Actinoallomurus A8, and its active form is produced by Streptomyces lividans TK24 through recombinant technology 22 . Latiglutenase is a combination of two glutenases, EP-B2 (From barley seed endosperm) and SC-PEP (A proline-specific endoprotease from Sphingomonas capsulata ) 23 . Also, E40 has been previously demonstrated to break down the highly immunogenic 33-mer peptide and the entire gliadin proteins, leading to a significant reduction in the release of interferon (IFN)-γ when exposed to T cells isolated from individuals with CeD 24 .…”

Section: Discussionmentioning

confidence: 99%

Bromelain-loaded nanocomposites decrease inflammatory and cytotoxicity effects of gliadin on Caco-2 cells and peripheral blood mononuclear cells of celiac patients

Mousavi Maleki,

Ebrahimi kiasari,

Seyed Mousavi

et al. 2023

Sci Rep

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Enzyme therapy can be an appropriate treatment option for celiac disease (CeD). Here, we developed Bromelain-Loaded Nanocomposites (BLNCs) to improve the stability and retention of bromelain enzyme activity. After the characterization of BLNCs, the cytotoxicity of BLNCs was determined on the Caco-2 cell line. The effect of BLNCs on gliadin degradation and the production of pro-inflammatory cytokines and anti-inflammatory molecules in peripheral blood mononuclear cells (PBMCs) obtained from celiac patients were assessed. Furthermore, the expression of CXCR3 and CCR5 genes was measured in CaCo-2 cells treated with gliadin, gliadin-digested with BLNCs, and bromelain. Our study demonstrated that the Bromelain entrapment efficiency in these nanoparticles was acceptable, and BLNCs have no toxic effect on cells. SDS-PAGE confirmed the digestion effect of bromelain released from nanocomposites. When Caco-2 cells were treated with gliadin digested by free bromelain and BLNCs, the expression of CXCR3 and CCR5 genes was significantly decreased. PBMCs of celiac patients treated with Bromelain and BLNCs decreased inflammatory cytokines (IL-1β, IL-6, TNF-α, and IFN-γ) production compared to untreated PBMCs. This treatment also increased IL-10 and CTLA-4 in PBMCs of CeD patients. According to the promising results of this study, we can hope for the therapeutic potential of BLNCs for CeD.

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Section: Discussionmentioning

confidence: 99%

Bromelain-loaded nanocomposites decrease inflammatory and cytotoxicity effects of gliadin on Caco-2 cells and peripheral blood mononuclear cells of celiac patients

Mousavi Maleki,

Ebrahimi kiasari,

Seyed Mousavi

et al. 2023

Sci Rep

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“…Currently, the most promising treatment is the vaccine Nexvax2, which is an adjuvant-free mixture of tripeptides immunodominant epitopes for gluten-specific CD4-positive T cells. However, it is still in the preliminary stages [160][161][162].…”

Section: Celiac Diseasementioning

confidence: 99%

Autoimmune Diseases of the GI Tract Part II: Emergence of Diagnostic Tools and Treatments

Al-Ruweidi¹,

Khater²,

Alkaabi³

et al. 2022

Immunology of the GI Tract - Recent Advances

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Autoimmune diseases (AD) have emerged as a pandemic in our modern societies, especially after the World War II. In part I, we have reviewed five main diseases and shed light on different aspects from introducing the concept of autoimmunity, the description of the disease’s pathogenesis and the diagnosis, the role of antibodies as markers for the prediction of the disease, the link between the gut and brain through what is known as the gut–brain axis, and the relationship of this axis in GI autoimmune diseases. In this chapter, we review the role of antibodies as markers for the prediction of the disease, artificial intelligence in GI autoimmune diseases, the nutritional role and implications in the five GI autoimmune diseases, and finally the treatment of those diseases.

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Non-dietary therapies for celiac disease

Cited by 2 publications

References 181 publications

Bromelain-loaded nanocomposites decrease inflammatory and cytotoxicity effects of gliadin on Caco-2 cells and peripheral blood mononuclear cells of celiac patients

Bromelain-loaded nanocomposites decrease inflammatory and cytotoxicity effects of gliadin on Caco-2 cells and peripheral blood mononuclear cells of celiac patients

Autoimmune Diseases of the GI Tract Part II: Emergence of Diagnostic Tools and Treatments

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