Non-disruptive measurement system of cell viability in bioreactors

Rudek, F.; Nelsen, Bryan; Baselt, Tobias; Berger, Tanja; Wiele, M.; Prade, Ina; Hartmann, Péter

doi:10.1117/12.2211545

SPIE Proceedings

2016

DOI: 10.1117/12.2211545

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Non-disruptive measurement system of cell viability in bioreactors

F. Rudek

Bryan Nelsen

Tobias Baselt

et al.

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2018

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“…12,13 The use of optical radiation as a measurement tool could alter or harm the cell culture, which limits the maximal usable intensity and measuring time. 14 We have developed a nondisruptive fast method of angle-resolved scattering measurement in cell culture plates, which enables the characterization of pathological cell changes during cultivation without influencing their growth. The pseudorandom scattering characteristics were attributed to particular cellular structures and used as a method to determine the state of induced necrosis of the cells.…”

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Supercontinuum-based nondisruptive scattering analyses of mouse fibroblast L929 cells before and after necrosis

et al. 2018

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Supercontinuum-based nondisruptive scattering analyses of mouse fibroblast L929 cells before and after necrosis," J.Abstract. The scattering properties of biological tissue are highly dependent on the structure size, refractive index, and wavelength of the incident light. Furthermore, these scattering characteristics are strongly influenced by movements of the scattering objects. A method is developed to determine the angular-and spectral-resolved scattering properties that enabled the characterization of biological nano-and microscaled cell structures. Nanosecond pulses from a spectrally filtered supercontinuum light source are captured and time-resolved to depress background noise and minimize disruptive effects of the biological cells. The scattering characteristics of a monolayer of mouse fibroblast L929 cells are measured at defined wavelengths in a standard cell culture plate. Because of the size and distribution of the scattering structures, a Fourier transform-based Mie scattering scheme is used to analyze the data. The system is tested to detect structural changes of mouse fibroblast L929 cells before and after poisoning with Triton X100. The final result is the development of a contamination-free method to study pathological changes in cell cultures, necrosis, or other cell-damaging effects.

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Supercontinuum-based nondisruptive scattering analyses of mouse fibroblast L929 cells before and after necrosis

et al. 2018

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Non-disruptive measurement system of cell viability in bioreactors

Cited by 1 publication

References 26 publications

Supercontinuum-based nondisruptive scattering analyses of mouse fibroblast L929 cells before and after necrosis

Supercontinuum-based nondisruptive scattering analyses of mouse fibroblast L929 cells before and after necrosis

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