Non‐electrolyte permeability of trout gills: effect of temperature and adrenaline

Isaia, J.

doi:10.1113/jphysiol.1979.sp012625

Cited by 12 publications

(6 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results presented indicate the existence of different pathways across the gills as a function of the physico-chemical properties of the non-electrolyte, and thus support the original hypothesis (Isaia, 1979) concerning different pathways for the various molecules: (a) a secondary lamellar pathway for small molecules, (b) a paracellular pathway for molecules the size of mannitol and (c) an extrusion pathway for hexose polymers in primary lamellae.…”

Section: Non-electrolyte Pathways In Trout Gillssupporting

confidence: 83%

“…Two results point to the complexity of the relationship between structure and function in the bronchial epithelium. (1) The gills possess a very impermeable epithelium compared to other tight epithelia (Isaia et al 1978 a) especially in sea water where shallow junctions exist between chloride cells (Isaia, 1979). (2) In the course of transfer from fresh water to sea water the bronchial permeability decreases for water, (Isaia, Payan & Girard, 1979), increases for mannitol and remains constant for dextran (Tables 2 and 3).…”

Section: Non-electrolyte Pathways In Trout Gillsmentioning

confidence: 99%

See 1 more Smart Citation

Effects of environmental salinity on branchial permeability of rainbow trout, Salmo gairdneri.

Isaia¹

1982

The Journal of Physiology

View full text Add to dashboard Cite

SUMMARY1. The gill fluxes of various non-electrolytes were measured in fresh-water-and sea-water-adapted trout (Salmo gairdneri). The studies were performed in vitro with a 'perfused-head' preparation.2. The results allow one to specify different transepithelial pathways according to the physico-chemical characteristics of the permeant molecules: (1) for hydrophilic and lipophilic molecules of small molecular radius, a transcellular pathway in the respiratory cells of the secondary lamellae, (2) for hexose, all paracellular pathways in the gill epithelium, and (3) for hexose polymers (inulin, dextran), a transcellular pathway in the chloride cells of the primary lamellae.3. The selectivity of the respiratory cells as a function of the liposolubility of the molecules tested is low. The decrease of this selectivity in the course of salt-water adaptation taken together with the modification of lipid composition of membranes and the effect of adrenaline on the bronchial permeability suggests that non-electrolytes diffuse through a lipid phase able to form hydrogen bonds.4. The high permeability of gills to hexose polymers of high molecular weight suggests a vesicular transport, especially in fish adapted to fresh water.

show abstract

Section: Non-electrolyte Pathways In Trout Gillssupporting

confidence: 83%

Section: Non-electrolyte Pathways In Trout Gillsmentioning

confidence: 99%

Effects of environmental salinity on branchial permeability of rainbow trout, Salmo gairdneri.

Isaia¹

1982

The Journal of Physiology

View full text Add to dashboard Cite

show abstract

“…Therefore, the decrease of PCP uptake rates with increasing salinity (Table 2), which is most prominent in the low salinity range, may be due to the decline of water flux accompanied by the increase of environmental salinity. During seawater acclimation, the gill permeability to small molecules such as ethanol and buthanol decreased, that to molecules the size of mannitol increased, and that to large molecules such as dextran stayed constant (Isaia 1979(Isaia , 1982. The developing chloride cell and its arm allows the tubular reticulum and extracellular space to communicate with the external milieu via leaky junctions (Sardet et al 1979).…”

Section: Discussionmentioning

confidence: 98%

The effects of salinity on pentachlorophenol accumulation and elimination by killifish (Oryzias latipes)

Tachikawa

Sawamura

1994

Arch. Environ. Contam. Toxicol.

View full text Add to dashboard Cite

Abstract. Fresh water and seawater acclimated (FWA and SWA) killifish (Oryzias latipes) were exposed to pentachlorophenol (PCP) at various salinities (1.2-18.7'/oo). The PCP accumulation in the fish decreased with increase of salinity. As the salinity increased [Woo (freshwater) -18.67o0], the PCP clearance rate increased from 0.0097 to 0.0170 (h-l). A substantial increase of the clearance rate was observed when the salinity was over 9.3?o0. The PCP uptake rate decreased from 20.8 to 10.6 (m1-1 g-1 fish h -1) with the increase of salinity. The uptake rate decreased at a lower salinity (~<4.7%o). The excretion of PCP glucuronide (PCP-G) increased markedly at the salinity of 9.3%o, although that of PCP sulfate (PCP-S) did not change. The increase of PCP-G excretion may correspond to the increase of clearance rate. Abrupt transfer of FWA and SWA killifish to a PCP solution with a different salinity decreased the PCP accumulation and increased the PCP metabolite excretion with the increase of the salinity. However, the amount of PCP accumulated by FWA was greater than that by SWA killifish.Aquatic habitats are divided into fresh, brackish and seawater environment. Many chemical pollutants are widely detected in these areas. Differences between seawater and freshwater accli= mated (SWA and FWA) fishes in accumulation and elimination of chemical pollutants have been reported (Canton et al. 1975(Canton et al. , 1978Tsuda et al. 1990;Tachikawa et al. 1987aTachikawa et al. , 1987bTachikawa et al. , 1991. These findings indicated that salinity can affect the accumulation and elimination of environmental pollutants in fish. Some studies have indicated that osmotic regulation in euryhaline fish is dependent on the salinity of the environment (Assem and Hanke 1979;Vislie and Fugelli 1984; PlazaYglesias et al. 1988;Hutchinson and Hawkins 1990). Examination of the uptake and clearance of chemicals associated with salinity changes in the environment may elucidate the effects of osmotic regulation in the accumulation and elimination of chemicals.Correspondence to: M. Tachikawa Previously, we reported the differences between FWA and SWA killifish Oryzias latipes in the accumulation and elimination of pentachlorophenol (PCP) (Tachikawa et al. 1991). Acclimation to seawater lowered the estimated bioconcentration factor (BCF) of PCP for killifish from 1680 to 370. The smaller uptake rate and the faster clearance rate resulted in the lower BCF of PCP for killifish in seawater. The greater excretion of PCP glucuronide by seawater killifish may be responsible for the rapid elimination of PCP.Herein, PCP accumulation, PCP uptake and clearance rates and PCP conjugates excretion by killifish acclimated to different salinities (1.2-18.7%o) were determined. Materials and Methods WaterFreshwater was reconstituted fresh water containing 26.1 mg CaC12 • 2H20, 18.2 mg MgSO 4 • 7H20, 1.1 nag K2SO4, and 25.3 mg NaHCO 3 in 1 L of deionized water. Seawater (salinity: 35%0) was reconstituted seawater by Lyman and Fleming (1940). The pH of the fresh w...

show abstract

“…Isaia etal. (1978b) and Isaia (1979) speculate that branchial permeability may be similarly increased to the respiratory gases oxygen, carbon dioxide and un-ionized ammonia. In freshwater fish, epinephrine also stimulates excretion of ionized ammonia, uptake of sodium and maintenance of acid-base balance (Girard & Pavan 1980).…”

Section: Qualitative Studies: Pathogenesis Of Branchial Lesionsmentioning

confidence: 97%

“…Stress-mediated release of epinephrine may be the key element in compensating for loss of surf ace area. Isaia, Girard & Payan (1978a), Isaia,Maetz& Haywood (1978b) and Isaia (1979) have shown that epinephrine increases branchial permeability to the lipophilic substance butanol and to water and urea. Isaia etal.…”

Section: Qualitative Studies: Pathogenesis Of Branchial Lesionsmentioning

confidence: 98%

Histological effects of the inert suspended clay kaolin on the gills of juvenile rainbow trout, Salmo gairdneri Richardson

Goldes

Ferguson

Moccia

et al. 1988

Journal of Fish Diseases

View full text Add to dashboard Cite

Juvenile rainbow trout were exposed to 36, 171, 1017 and 4887 mg/1 of suspended kaolin clay for 64 days. Gills were analysed quahtatively and quantitatively using histology. Branchial pathology was absent at all sample times in fish exposed to 36,171 and 1017 mg/! kaolin, althoughintracellularkaotinwas visible within filament and lamellar epithelium in gills exposed to 171, 1017 and 4887 mg/1 kaolin at 4, 8, 16, 32 and 64 days. Lesions were found in gills exposed to 4887 mg/I kaolin at 16 and 32 days but the marked lamellar proliferation was associated with moderate to large numbers of protozoa identified as Ichthyobodo necator. Gills regained normal architecture by 64 days despite continued exposure to kaolin. It is suggested that while the gill lesions were probably a direct result of protozoan infection, kaolin was indirectly responsible for creating a favourable environment for protozoan colonization. The recovery of normal architecture and reduction of protozoan numbers between 32 and 64 days was probably a function of increased mucus secretion, immune response and turnover of branchial epithelium. The authors conclude that exposure to prevailing levels of this inert clay particle, even for periods as long as 64 days, probably has little direct effect on gill structure at the light microscope level. The action of noninert and organic particles remains to be investigated.

show abstract

Non‐electrolyte permeability of trout gills: effect of temperature and adrenaline

Cited by 12 publications

References 32 publications

Effects of environmental salinity on branchial permeability of rainbow trout, Salmo gairdneri.

Effects of environmental salinity on branchial permeability of rainbow trout, Salmo gairdneri.

The effects of salinity on pentachlorophenol accumulation and elimination by killifish (Oryzias latipes)

Histological effects of the inert suspended clay kaolin on the gills of juvenile rainbow trout, Salmo gairdneri Richardson

Contact Info

Product

Resources

About