Non-enzymatic detection of glucose in fruits using TiO2–Mn3O4 hybrid nano interface

Abstract

Consumption of fruits leads to increase in glucose level in blood for diabetic patients, which in turn leads to peripheral, vascular, ocular complications and cardiac diseases. In this context, a non-enzymatic hybrid glucose biosensor was fabricated for the first time to detect glucose by immobilizing titanium oxide-manganese oxide (TiO 2 -Mn 3 O 4 ) nanocomposite and chitosan membrane on to the surface of Pt working electrode (Pt/TiO 2 -Mn 3 O 4 /chitosan). TiO 2 -Mn 3 O 4 nanocomposite catalyzed the oxidation of glucose to gluconolactone in the absence of glucose oxidase enzyme with high electron transfer rate, good biocompatibility and large surface coverage. Electrochemical measurements revealed the excellent sensing response of the developed biosensor towards glucose with a high sensitivity of 7.073 lA mM -1 , linearity of 0.01-0.1 mM, low detection limit of 0.01 lM, reproducibility of 1.5% and stability of 98.8%. The electrochemical parameters estimated from the anodic process were subjected to linear regression models for the detection of unknown concentration of glucose in different fruit samples.

Guide to Selecting a Biorecognition Element for Biosensors

“…25,50,56 The nanostructured surface can have high sensitivity but poor specificity, as multiple bioanalytes can be simultaneously catalytically activated. 57–59 Even though progress has been made to improve the selectivity of biorecognition element mimics with nanozymes, this sensor paradigm remains less selective than the other discussed paradigms within this review. 25 One promising application of nanostructured surfaces includes integrating multiple catalytic surfaces to create a cross-reactive (e.g.…”

“…Another approach to detecting bioanalytes is to use synthetic surface nanostructures to catalytically activate a bioanalyte without the need of a natural biorecognition element. − As an example, nanozymes mimic enzymatic catalytic function by interfacing with a recognition element mimic and an inorganic catalysts. ,, The nanostructured surface can have high sensitivity but poor specificity, as multiple bioanalytes can be simultaneously catalytically activated. − Even though progress has been made to improve the selectivity of biorecognition element mimics with nanozymes, this sensor paradigm remains less selective than the other discussed paradigms within this review . One promising application of nanostructured surfaces includes integrating multiple catalytic surfaces to create a cross-reactive (e.g., profile-based) sensors. − However, clinicians struggle with profile-based sensing because it does not identify specific analytes in the traditional biosensor paradigm and definition …”

“Nano”: An Emerging Avenue in Electrochemical Detection of Neurotransmitters

“…Single nanomaterials have earlier been explored as interface materials. Recently, combinations of these nanomaterials have been used as hybrid interfaces − that serve to further enhance the sensing properties in terms of sensitivity and help to increase the dynamic range of detection. These parameters become invaluable in the field of clinical diagnostics.…”

Synthesis and Application of Graphdiyne Oxide-Polyurethane Nanocomposite Yield a Highly Sensitive Non-Enzyme Glucose Sensor

“…[4][5][6][7] Various membranes, polymers and nanocomposites, that exhibit large electroactive surface areas and high electrocatalytic activities, can serve as sensitive recognition units in non-enzymatic glucose sensors. 8,9 Carbon nanomaterials with structural uniformity, such as reduced graphene oxide, noble metal (Au, Ag, Pt, Pd, etc) carbon nanocomposite, [10][11][12][13] transition metal oxides (Cu 2 O, NiO, SiO 2 , etc.) [14][15][16][17][18][19] or conducting polymers (polyaniline, polyacrylamide, etc) have been the widely studied towards electrochemical sensing.…”