Non-Enzymatic Electrochemical Detection of Urine Creatinine Using Cobalt-Gold Bimetallic Nanoparticles

Abstract

This work presents the development of a non-enzymatic electrochemical sensor for creatinine in a neutral medium using a cobalt-gold bimetallic nanoparticles modified platinum electrode. The voltammetric detection of creatinine in a neutral phosphate buffer was based on the formation of a soluble cobalt-creatinine complex. The sensor exhibited good selectivity and a detection limit (S/N = 3) of 2.25 mM with two linear ranges from 6.4 to 83.2 mM. The sensitivity of the sensor was 0.621 and 1.135 μA mM-1 cm-2 at lower (6.4 – 51.2 mM) and higher (51.2 – 83.2 mM) detection ranges, respectively. The sensor performance was validated using urine samples, and creatinine spiked urine samples.