Non-enzymatic glycation and oxidative stress in chronic disease and diabetes mellitus

Nawroth, Peter P.; Bierhaus, Angelika; Vogel, Gabriele E.; Hofmann, M.; Zumbach, M.; Wahl, Peter; Ziegler, Reinhard

doi:10.1007/bf03044692

Cited by 23 publications

(13 citation statements)

References 139 publications

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“…RAGE is a primary cell surface receptor for the S100/calgranulin ligand superfamily and EN-RAGE (extracellular newly identified RAGE-binding protein) (Nawroth et al, 1999). Cellular RAGE can bind with EN-RAGE in the endothelium on mononuclear phagocytes or lymphocytes to trigger cellular activation with the generation of key proinflammatory mediators such as TNF-a and CRP .…”

Section: Discussionmentioning

confidence: 99%

Association of Polymorphisms of the Receptor for Advanced Glycation End Products Gene with COPD in the Chinese Population

Cheng²,

Ma³

et al. 2014

DNA and Cell Biology

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The receptor for advanced glycation end products (RAGE) is a cell surface molecule of the immunoglobulin superfamily that binds diverse endogenous ligands involved in the development of chronic diseases and inflammatory damage. A growing body of evidence has suggested that RAGE is involved in the development and progression of chronic obstructive pulmonary disease (COPD). The present study investigated the existence of an association among three polymorphisms (-374T/A, -429T/C, and G82S) of the RAGE gene with the risk of COPD in the Chinese population. The RAGE genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism in 216 patients with COPD and 239 age-matched healthy individuals. Our study demonstrated that the frequencies of the GS genotype and the S allele in the G82S mutation were significantly higher in COPD patients than in controls (odds ratios [OR]=1.70, 95% confidence interval [CI]: 1.15-2.50, p=0.0098 and OR=1.42, 95% CI: 1.06-1.91, p=0.023, respectively). Further stratification analysis by smoking status revealed that the presence of the GS genotype conferred a higher risk of developing COPD in current smokers (p=0.044). In contrast, mutations at -374T/A and -429T/C did not demonstrate any association with COPD, even after taking into account the patients' smoking history. Our study provides preliminary evidence that the G82S polymorphism in the RAGE gene is associated with an increased risk of COPD and that the GS genotype of the G82S variant is a risk factor for COPD in the Chinese population.

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Section: Discussionmentioning

confidence: 99%

Association of Polymorphisms of the Receptor for Advanced Glycation End Products Gene with COPD in the Chinese Population

Cheng²,

Ma³

et al. 2014

DNA and Cell Biology

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“…Although both genetic and environmental factors appear to play a role, the cause of diabetes mellitus is still not clear. A large number of studies have demonstrated that oxidative stress and nonenzymatic protein glycation are closely associated with the development of diabetes mellitus (Nawroth et al, 1999;Vlassara and Palace, 2002). Antioxidants protect against glycationderived free radicals and may have therapeutic potential (Ceriello et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and antiglycation properties of total saponins extracted from traditional Chinese medicine used to treat diabetes mellitus

Hai

Tang

et al. 2007

Phytotherapy Research

123

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Eleven antidiabetic traditional Chinese medicine (TCM) extracts rich in saponins were examined for their antioxidant and antiglycation activities. The antioxidant activities of these extracts were evaluated by studying the inhibition of lipid peroxidation in rat liver microsomes induced by ascorbate/Fe2+, cumine hydroperoxide (CHP) or CCl4/reduced form of nicotinamide-adenine dinucleotide phosphate (NADPH). The antioxidant capacities were also evaluated by studying the scavenging of 2,2'-diphenyl-1-picrylhydrazyl (DPPH) free radical. The antiglycation activities of these extracts were evaluated by hemoglobin-delta-gluconolactone (delta-Glu) assay, bovine serum albumin (BSA)-glucose assay and N-acetyl-glycyl-lysine methyl ester (GK peptide)-ribose assay. Aralia taibaiensis outperformed other extracts in most of the assays except inhibition of early glycation products formation, where Acanthopanax senticosus showed higher activity. Aralia taibaiensis was particularly potent in inhibiting the late glycation and formation of advanced glycation end products (AGEs) on proteins. The antioxidant and antiglycation activities of most extracts were correlated with the saponin content. The results demonstrate that the antidiabetic activities of most extracts could be explained, at least in part, by their combined antioxidant and antiglycation properties.

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“…The majority of studies on non‐enzymatic glycosylation have been carried out with higher eukaryotes, particularly humans, because of the clinical importance of this process and its relation to ageing (Chellan and Nagaraj, 1999; Nawroth et al ., 1999). There are only a few reports on non‐enzymatic glycosylation in lower eukaryotes.…”

Section: Introductionmentioning

confidence: 99%

Evidence for non‐enzymatic glycosylation in Escherichia coli

Mironova

Niwa

Hayashi

et al. 2001

Molecular Microbiology

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SummaryNon-enzymatic glycosylation (glycation) is a chain of chemical reactions affecting free amino groups in proteins of long-living eukaryotes. It proceeds in several steps leading to the consecutive formation of Schiff bases, Amadori products and advanced glycation end-products (AGEs). To our knowledge, this process has not been observed in prokaryotes so far. However, the present study provides clear-cut evidence that glycation takes place in bacteria despite their short life span. We have detected AGEs in recombinant human interferon gamma (rhIFN-g) produced in Escherichia coli as well as in total protein of the same bacterium using three different approaches: (i) Western blotting using two monoclonal antibodies raised against AGEs; (ii) fluorescent spectroscopy; and (iii) investigation of the effect of known AGE inhibitors (such as acetyl salicylic acid and thiamine) on the glycation reaction. Our study shows that nonenzymatic glycosylation is initiated during the normal growth of E. coli and results in AGE formation even after isolation of proteins. This process seems to be tightly associated with some post-translational modifications observed in the cysteineless rhIFN-g, such as covalent dimerization and truncation.

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Non-enzymatic glycation and oxidative stress in chronic disease and diabetes mellitus

Cited by 23 publications

References 139 publications

Association of Polymorphisms of the Receptor for Advanced Glycation End Products Gene with COPD in the Chinese Population

Association of Polymorphisms of the Receptor for Advanced Glycation End Products Gene with COPD in the Chinese Population

Antioxidant and antiglycation properties of total saponins extracted from traditional Chinese medicine used to treat diabetes mellitus

Evidence for non‐enzymatic glycosylation in Escherichia coli

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