Non‐hybridization single nucleotide polymorphism detection and genotyping assay through direct discrimination of single base mutation by capillary electrophoretic separation of single‐stranded DNA

Takahashi, Toru; Fukagawa, Mayu; Sakurai, Takao; Hoshino, Hitoshi

doi:10.1002/jssc.201900897

Cited by 2 publications

(1 citation statement)

References 15 publications

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“…An advantage that the F-108 CE-SSCP method has over SNaPshot™ is that a purification step is not necessary before electrophoresis, making the method less costly per sample, and also less time consuming [16]. Another CE method distinguished single base-pair mutations in the same length fragments by utilizing the DNA base protonation properties [21,22]. However, in this method, additional reagents, such as a PEO polymer, acidic buffer, and urea, are required.…”

Section: Resultsmentioning

confidence: 99%

Resolving human DNA mixtures with F‐108 polymer and capillary electrophoresis single‐strand conformational polymorphism analysis

Jordan

Mills

2020

J of Separation Science

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Current technologies have increased the sensitivity for analyzing forensic DNA samples, especially those considered “touch samples.” Because of this, there has been an increase in the number of forensic mixtures–two or more contributors within a single sample–submitted to the crime laboratories. Therefore, the need to resolve these mixtures has increased as well. Several technologies are currently utilized, but many of them are time consuming and do not resolve the entire profile. Therefore, CE‐Single‐Strand Conformational Polymorphisms coupled with the Pluronic F‐108 polymer was assessed for its ability to resolve human forensic mixtures. This technique has been able to detect sequence variation, such as single nucleotide polymorphism in short tandem repeat loci, such as D7S820 and vWA. Samples were first analyzed with the Performance Optimized Polymer‐7, and mixtures created from samples that shared alleles. These samples were sequenced to detect single base‐pair mutations and evaluated with the F‐108 and CE‐Single Strand Conformational Polymorphism analysis. Results from this study indicated the method would serve as a valuable screening tool to detect base sequence variation between individuals when they share alleles in a mixture and before using Massive Parallel Sequencing technology to distinguish which bases differ.

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Section: Resultsmentioning

confidence: 99%

Resolving human DNA mixtures with F‐108 polymer and capillary electrophoresis single‐strand conformational polymorphism analysis

Jordan

Mills

2020

J of Separation Science

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Progress in Protein Modification of Selectin and Its Relationship with Chronic Inflammatory Diseases

黄

2023

ACM

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Non‐hybridization single nucleotide polymorphism detection and genotyping assay through direct discrimination of single base mutation by capillary electrophoretic separation of single‐stranded DNA

Abstract: capillary electrophoresis, deoxyribonucleic acid, genotyping, single nucleotide polymorphism J Sep Sci 2020;43:657-662.

Cited by 2 publications

References 15 publications

Resolving human DNA mixtures with F‐108 polymer and capillary electrophoresis single‐strand conformational polymorphism analysis

Resolving human DNA mixtures with F‐108 polymer and capillary electrophoresis single‐strand conformational polymorphism analysis

Progress in Protein Modification of Selectin and Its Relationship with Chronic Inflammatory Diseases

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