Non-Invasive Analysis of Human Liver Metabolism by Magnetic Resonance Spectroscopy

Jones, John G.

doi:10.3390/metabo11110751

Cited by 6 publications

(5 citation statements)

References 112 publications

(191 reference statements)

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“…Проблемы и потенциальные ограничивающие факторы широкого применения фосфорной МР-спектроскопии в клинической практике включают разработку и внедрение радиочастотного оборудования для оптимального наблюдения за печеночными метаболитами, нивелирования смешанных сигналов от других тканей, находящихся в тесном контакте с печенью, таких как фосфатидилхолин из желчного пузыря и фосфокреатин из окружающих мышц. В научных публикациях встречаются исследования печени, которые объединяют результаты фосфорной и протонной МР-спектроскопии, тем самым предоставляя коррелированную информацию о фосфометаболитах и липидном спектре [26].…”

Section: радионуклидный методunclassified

Methods of radiology in the diagnostics of chronic liver diseases

Savchenkov,

Trufanov,

Fokin

et al. 2024

vmirvz

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Background. Chronic liver disease is one of the most common diseases. In many countries, liver disease is among the top five causes of death. The liver is one of the main organs responsible for basic metabolic functions, protein and hormone synthesis, detoxification and waste elimination. In chronic liver disease, there is a continuous process of inflammation, destruction and regeneration, ultimately leading to severe dysfunction, causing the development of fibrosis and cirrhosis. The main task of the radiation diagnosis of chronic liver disease is the development and introduction into clinical practice of new noninvasive biomarkers for a comprehensive assessment of the structure of the liver parenchyma in order to choose further treatment tactics.Aim a comprehensive analysis of the modern possibilities of radiation imaging methods in the diagnosis of chronic liver disease.Materials and methods. The analysis of 107 modern publications of domestic and foreign literature devoted to the diagnosis of chronic liver disease of various etiologies was carried out.Conclusion. the review reflects the most common modern and promising methods of radiodiagnosis for chronic liver disease, which in most cases make it possible to avoid invasive interventions in the process of establishing a diagnosis and monitoring the response to treatment

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Section: радионуклидный методunclassified

Methods of radiology in the diagnostics of chronic liver diseases

Savchenkov,

Trufanov,

Fokin

et al. 2024

vmirvz

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“…[1][2][3][4] Due to limited accessibility or need for invasive procedures, metabolic imaging is a promising method for in vivo investigations of liver metabolism in humans, in particular the dynamic monitoring of intrahepatic lipids, hepatic glucose and its storage in the form of glycogen. In this context, MRI and MRS [5][6][7] using proton, carbon, and deuterium MRS are ideally suited. To maximize convenience and overcome the challenge of reproducing metabolic situations (e.g., influence of nutrition and activity), investigations of lipid and glucose metabolism are ideally integrated within a single-session examination.…”

Section: Introductionmentioning

confidence: 99%

“…8 1 H-MRS is also especially useful for determination of lipid composition. 5 Hepatic lipid content exhibits a significant correlation with insulin resistance in vivo, which serves as the best predictor for the development of Type 2 diabetes (T2DM) and is a hallmark of metabolic dysfunction-associated fatty liver disease (MAFLD) or nonalcoholic fatty liver disease (NAFLD). 9 Hepatic lipid content quantification with 1 H-MRS thus provides data that closely correlate with biochemical and histologic hepatic lipid storage analysis and has therefore unparalleled sensitivity.…”

Section: Introductionmentioning

confidence: 99%

“…Single‐voxel 1 H‐MRS has become the gold standard for noninvasive quantification of hepatic lipid content, covering the whole range from very low lipid content encountered in young lean healthy subjects all the way to full‐blown hepatic steatosis, while Dixon‐type MRI is most useful for the pathologic range of lipid content 8 . 1 H‐MRS is also especially useful for determination of lipid composition 5 . Hepatic lipid content exhibits a significant correlation with insulin resistance in vivo, which serves as the best predictor for the development of Type 2 diabetes (T2DM) and is a hallmark of metabolic dysfunction‐associated fatty liver disease (MAFLD) or nonalcoholic fatty liver disease (NAFLD) 9 .…”

Section: Introductionmentioning

confidence: 99%

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Interleaved trinuclear MRS for single‐session investigation of carbohydrate and lipid metabolism in human liver at 7T

Poli,

Emara,

Lange

et al. 2024

NMR in Biomedicine

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The liver plays a central role in metabolic homeostasis, as exemplified by a variety of clinical disorders with hepatic and systemic metabolic disarrays. Of particular interest are the complex interactions between lipid and carbohydrate metabolism in highly prevalent conditions such as obesity, diabetes, and fatty liver disease. Limited accessibility and the need for invasive procedures challenge direct investigations in humans. Hence, noninvasive dynamic evaluations of glycolytic flux and steady‐state assessments of lipid levels and composition are crucial for basic understanding and may open new avenues toward novel therapeutic targets. Here, three different MR spectroscopy (MRS) techniques that have been combined in a single interleaved examination in a 7T MR scanner are evaluated. 1H‐MRS and 13C‐MRS probe endogenous metabolites, while deuterium metabolic imaging (DMI) relies on administration of deuterated tracers, currently 2H‐labelled glucose, to map the spatial and temporal evolution of their metabolic fate. All three techniques have been optimized for a robust single‐session clinical investigation and applied in a preliminary study of healthy subjects. The use of a triple‐channel 1H/2H/13C RF coil enables interleaved examinations with no need for repositioning. Short‐echo‐time STEAM spectroscopy provides well resolved spectra to quantify lipid content and composition. The relative benefits of using water saturation versus metabolite cycling and types of respiratory synchronization were evaluated. 2H‐MR spectroscopic imaging allowed for registration of time‐ and space‐resolved glucose levels following oral ingestion of 2H‐glucose, while natural abundance 13C‐MRS of glycogen provides a dynamic measure of hepatic glucose storage. For DMI and 13C‐MRS, the measurement precision of the method was estimated to be about 0.2 and about 16 mM, respectively, for 5 min scanning periods. Excellent results were shown for the determination of dynamic uptake of glucose with DMI and lipid profiles with 1H‐MRS, while the determination of changes in glycogen levels by 13C‐MRS is also feasible but somewhat more limited by signal‐to‐noise ratio.

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“…The liver is the central organ involved in fatty acid metabolism. The liver stores fatty acids as triglycerides in hepatocytes and metabolizes fatty acids through beta-oxidation in the mitochondria of hepatocytes [ 5 , 6 ]. The expression of SGLT2 has been observed in various human hepatoma cell lines [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Hepatic expression of sodium–glucose cotransporter 2 (SGLT2) in patients with chronic liver disease

et al. 2022

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Sodium–glucose cotransporter 2 (SGLT2) occurs in the proximal renal tubule cells. We investigate the hepatic expression of SGLT2 and its related factors in patients with chronic liver disease. This is a retrospective human study. The liver tissues were biopsied from patients with chronic liver disease (n = 30). The expression levels of SGLT2 were evaluated by immunostaining. Furthermore, the undirected graphical model was used to identify factors associated with hepatic expression levels of SGLT2. The SGLT2 expression was observed in not only the kidney, but also the liver in immunostaining (SGLT2 intensity: kidney 165.8 ± 15.6, liver 114.4 ± 49.0 arbitrary units, P < 0.01) and immunoblotting. There was no significant difference in hepatic expression of SGLT2 in the stratified analysis according to age, sex, BMI, and the severity of the liver disease. In the undirected graphical model, SGLT2 directly interacted with various factors such as sex, fatty change, neutrophil-to-lymphocyte ratio, triglyceride, hemoglobin A1c, creatinine, and albumin (partial correlation coefficient 0.4–0.6 for sex and 0.2–0.4 for others). The expression of SGLT2 was observed in the hepatocytes of patients with chronic liver disease. The undirected graphical model demonstrated the complex interaction of hepatic expression levels of SGLT2 with gender, inflammation, renal function, and lipid/glucose/protein metabolisms.

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Non-Invasive Analysis of Human Liver Metabolism by Magnetic Resonance Spectroscopy

Cited by 6 publications

References 112 publications

Methods of radiology in the diagnostics of chronic liver diseases

Methods of radiology in the diagnostics of chronic liver diseases

Interleaved trinuclear MRS for single‐session investigation of carbohydrate and lipid metabolism in human liver at 7T

Hepatic expression of sodium–glucose cotransporter 2 (SGLT2) in patients with chronic liver disease

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