2010
DOI: 10.2174/156802610791384234
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Non-Invasive Cell Tracking in Cancer and Cancer Therapy

Abstract: Cell-based therapy holds great promise for cancer treatment. The ability to non-invasively track the delivery of various therapeutic cells (e.g. T cells and stem cells) to the tumor site, and/or subsequent differentiation/proliferation of these cells, would allow better understanding of the mechanisms of cancer development and intervention. This brief review will summarize the various methods for non-invasive cell tracking in cancer and cancer therapy. In general, there are two approaches for cell tracking: di… Show more

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Cited by 82 publications
(63 citation statements)
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“…9,[21][22][23] Standardized protocols used for labeling stem cells with SPIONs were previously compiled by us, 15,24 and other direct-labeling reagents were reviewed by Marks and Nolan 25 and Progatzky et al 26 Indirect labeling is a considerably different method, which includes genetic modification in order to either produce an appropriate signal-generating molecule or increase the affinity of cells to contrast agents. 9,21,[27][28][29][30][31][32] Transient expression of reporter proteins by DNA vector transfection is often included in this set of cell labeling. 9 Another alternative is stable expression of the reporter protein by transduction of the cells with a virus.…”
Section: Labeling Stem Cells and Molecular Imaging Methodsmentioning
confidence: 99%
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“…9,[21][22][23] Standardized protocols used for labeling stem cells with SPIONs were previously compiled by us, 15,24 and other direct-labeling reagents were reviewed by Marks and Nolan 25 and Progatzky et al 26 Indirect labeling is a considerably different method, which includes genetic modification in order to either produce an appropriate signal-generating molecule or increase the affinity of cells to contrast agents. 9,21,[27][28][29][30][31][32] Transient expression of reporter proteins by DNA vector transfection is often included in this set of cell labeling. 9 Another alternative is stable expression of the reporter protein by transduction of the cells with a virus.…”
Section: Labeling Stem Cells and Molecular Imaging Methodsmentioning
confidence: 99%
“…Differently from transient expression, stably transformed cells will continue to produce the protein of interest for long periods and allow us to monitor not only its biodistribution and cell fate but also cell proliferation, considering that the daughter cells will also produce the marker. 21,32 Indirect and direct methods might serve the purpose of successfully labeling stem cells for noninvasive tracking in vivo. Table 1 summarizes the advantages and disadvantages of each method.…”
Section: Labeling Stem Cells and Molecular Imaging Methodsmentioning
confidence: 99%
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“…These methods have not been fully developed for the study of sex hormone signal in vivo; however, we expect that BRET and FRET, when combined with intravital microscopy or optical detection systems, will significantly improve the spatio-temporal analysis of hormonal pathways from single cell to a whole body resolution. More information are also expected to come from the application of imaging onto cancer stem cell field that already gave promising results (Hong et al 2010); imaging might help answering several open questions about the role of sex hormones on the fate of cancer stem cells (LaMarca & Rosen 2008). This is a rather important issue, which needs clarification to understand the mechanisms underlying the early events occurring during hormonal transformation and in the establishment of resistance.…”
Section: Discussionmentioning
confidence: 99%