Non-invasive delivery of nanoparticles to hair follicles: A perspective for transcutaneous immunization

Mittal, Ankit; Raber, Anne S.; Schaefer, Ulrich F.; Weißmann, Sebastian; Ebensen, Thomas; Schulze, Kai; Guzmán, Carlos A.; Lehr, Claus‐Michael; Hansen, Steffi

doi:10.1016/j.vaccine.2012.12.048

Cited by 77 publications

(53 citation statements)

References 52 publications

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“…This change in TSLP level may influence DC and T cell activation (Kinoshita et al, 2009;Spadoni et al, 2012). At the same time, since DCs can potentially be exposed directly to changes of sebum, chemical milieu and microbiota of the skin, the disruption of the non-inflammatory milieu can also be initiated by DCs or T cells (Mittal et al, 2013;Naik et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Sebaceous Gland-Rich Skin Is Characterized by TSLP Expression and Distinct Immune Surveillance Which Is Disturbed in Rosacea

Dajnoki

Béke

Kapitány

et al. 2017

Journal of Investigative Dermatology

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The microbial community exhibits remarkable diversity on topographically distinct skin regions, which may be accompanied by differences in skin immune characteristics. Our aim was to compare the immune milieu of healthy sebaceous gland rich (SGR) and sebaceous gland poor (SGP) skin areas, and to analyze its changes in an inflammatory disease of SGR skin. For this purpose, immunohistochemical, immunocytochemical and quantitative real-time PCR analyses of thymic stromal lymphopoietin (TSLP) and other cytokines, phenotypic immune cell markers and transcription factors were carried out in samples from SGP, SGR skin and from papulopustular rosacea (PPR). TSLP mRNA and protein production was also studied in cultured keratinocytes. In SGR skin, higher TSLP expression, dendritic cell (DC) appearance without prominent activation and T cell presence with interleukin (IL)-17/IL-10 cytokine milieu were detected compared to SGP skin. Linoleic acid, a major sebum component, was found to induce TSLP expression dose-dependently in keratinocytes. In PPR, significantly decreased TSLP level and influx of inflammatory DCs and T cells with IL-17/interferon-γ cytokine milieu were observed. According to our results, SGR skin is characterized by a distinct, non-inflammatory immune surveillance, which may explain the preferred localization of inflammatory skin diseases, and can influence future barrier repair therapeutic concepts.3

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Section: Discussionmentioning

confidence: 99%

Sebaceous Gland-Rich Skin Is Characterized by TSLP Expression and Distinct Immune Surveillance Which Is Disturbed in Rosacea

Dajnoki

Béke

Kapitány

et al. 2017

Journal of Investigative Dermatology

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“…We observed 2-3 times higher antigen amounts in the hair follicles of excised pig ear skin, when the antigen had been encapsulated in NPs as compared to antigen applied as solution. 5 However, TF vaccination is usually performed after pre-treating the skin area by plucking hairs, waxing or cyanoacrylate (superglue) stripping. [6][7][8] Pre-treatment not only permeabilizes the skin by removing the upper layers of the SC, but also activates the innate immune system.…”

Section: Introductionmentioning

confidence: 99%

“…As a proof of concept, we showed the successful delivery of antigen via the TF route using antigen-loaded NPs in an adoptive transfer mouse model by measuring the proliferation of antigen-specific CD4+ T cells. 5 Although the adoptive transfer model demonstrated the viability of this vaccination route, the experimental setting does not resemble a true vaccination. The proliferation of ovalbumin (OVA)-specific CD4+ T cells provides a hint on the feasibility of this approach.…”

Section: Introductionmentioning

confidence: 99%

Efficient nanoparticle-mediated needle-free transcutaneous vaccination via hair follicles requires adjuvantation

Mittal

Schulze

Ebensen

et al. 2015

Nanomedicine: Nanotechnology, Biology and Medicine

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In vaccine development, activating the immune system is the major challenge. In this perspective, transcutaneous immunization (TCI) offers an attractive approach due to the presence of abundant antigen presenting cells (APCs) such as Langerhans cells and dendritic cells. In particular transfollicular vaccination aims to deliver antigens to the abundant perifollicular APCs without compromising the SC barrier function. The present work clearly demonstrates the potential of transfollicular immunization as an approach to deliver antigens across intact skin following co-administration of nanoparticles with an adjuvant on intact skin as well as stimulating and modulating efficient humoral and cellular immune response according to the specific clinical needs. Conflict of interest:Claus Prior presentations:Parts of this study were presented on international conferences as listed below. There is no conflict of copy rights with these abstracts or proceedings.

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“…The use of hair follicle as selective targeting tool for dermatotherapy represents also a remarkable opportunity for the developments of novel strategies for dermocosmetics, and immunotherapy (Mittal et al, 2013;Vogt et al, 2005). The diffusion of polymeric nanoparticles into the hair follicle has been well described, as well as, its storage into the follicle even for ten days (Lademann et al, 2007).…”

Section: Skinmentioning

confidence: 99%

Polymeric Nanoparticles in Dermocosmetic

et al. 2015

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SUMMARY: Recent advances in the fields of biomaterials and nanotechnology have allowed the development of advanced nanoparticles for biomedical applications. Despite a vast number of nanostructures such as liposomes, solid-lipid nanocapsules, polymeric and hybrid lipid-polymer nanoparticles have been studied as carriers for drug delivery for different pathologies with remarkable promising results; the use of polymeric nanoparticles in dermocosmetic still has not been widely explored. The evolution of cosmetic into the care skin and dermatology represents novel technological challenges. Also, the increasing knowledge about normal skin physiology and advances in nanotechnology provide an attractive environment for the creation of innovative dermocosmetic formulations. In this work, we discuss the state of the art of polymeric nanoparticles formulated for dermocosmetics, its mechanisms of action, and diffusion into the skin.

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Non-invasive delivery of nanoparticles to hair follicles: A perspective for transcutaneous immunization

Cited by 77 publications

References 52 publications

Sebaceous Gland-Rich Skin Is Characterized by TSLP Expression and Distinct Immune Surveillance Which Is Disturbed in Rosacea

Sebaceous Gland-Rich Skin Is Characterized by TSLP Expression and Distinct Immune Surveillance Which Is Disturbed in Rosacea

Efficient nanoparticle-mediated needle-free transcutaneous vaccination via hair follicles requires adjuvantation

Polymeric Nanoparticles in Dermocosmetic

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