Non-invasive detection of iron deficiency by fluorescence measurement of erythrocyte zinc protoporphyrin in the lip

Hennig, Georg; Homann, Christian; Teksan, Ilknur; Hasbargen, Uwe; Hasmüller, Stephan; Holdt, Lesca M.; Khaled, N.; Sroka, Ronald; Stauch, Thomas; Stepp, Herbert; Vogeser, Michael; Brittenham, Gary M.

doi:10.1038/ncomms10776

Cited by 41 publications

(32 citation statements)

References 39 publications

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“…Other markers of iron deficiency, such as percent hypochromic red cells and reticulocyte haemoglobin (Ret He), can be used as additional tests to help the diagnosis in inflammatory states, and more sensitive tests are quoted in research studies, including zinc protoporphyrin, or soluble transferrin receptor assays, but these are not readily available for clinical use (Thomas et al, June 2013). A novel non-invasive assay measuring zinc protoporphyrin optically from the lower lip has been proposed and would be of use in resource limited settings but awaits further validation (Hennig et al, 17 February 2016). More recently, serum hepcidin has emerged as a useful adjunct in the assessment of iron status (Fig.…”

Section: Fig 2 Genetic and Acquired Factors Influencing Serum Hepcimentioning

confidence: 99%

The management of anaemia and haematinic deficiencies in pregnancy and post‐partum

Roy

Pavord

2018

Transfusion Medicine

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Anaemia is one of the most common disorders in the world (24·8% of the world population) (de Benoist 2008) and affects patients of all ages and ethnic origins. Underlying causes and prevalences vary by age group and socioeconomic background, but pregnant women everywhere are at high risk of anaemia, the vast majority of cases being due to iron deficiency. One in four pregnant women in Europe are thought to have iron deficiency anaemia (Daru et al., March 2016), whereas in parts of Africa, where hookworm infestation is common, this has been estimated to be as high as 38% (Stevens et al., 2013) to 50% (Bah et al., June 2017). Women of menstruating age are rarely iron replete (Low et al., 18 April 2016) and then enter pregnancy, which carries a major negative iron balance (Bentley, October 1985). Despite a good understanding of the causes of anaemia in pregnancy, there is still uncertainty about how best this should be investigated, prevented and managed. This reflects the limitations of laboratory tests, as well as the poor understanding of how best to replace iron, given the complex physiological mechanisms of iron absorption and distribution. A strategy for iron replacement in a population of anaemic pregnant women needs to be developed not only based on what is biologically and clinically most appropriate but also in the context of each organisation's delivery of care structure, taking into consideration aspects of cost effectiveness. For this reason, management algorithms must be adapted locally, ensuring they meet basic clinical imperatives.

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Section: Fig 2 Genetic and Acquired Factors Influencing Serum Hepcimentioning

confidence: 99%

The management of anaemia and haematinic deficiencies in pregnancy and post‐partum

Roy

Pavord

2018

Transfusion Medicine

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“…If iron is unavailable, divalent zinc is incorporated into PPIX instead of iron, producing zinc protoporphyrin (ZnPP), which is excited by 405 nm beams and emits at approximately 630 nm, similarly to PPIX 33 . ZnPP persists throughout the life span of RBCs as a biochemical indicator of functional iron deficiency 34 , 35 . Because our methodology does not distinguish ZnPP from PPIX, the XN-30 analyser implemented with the RBO channel possibly detected RBCs containing ZnPP but not PPIX in the in vivo studies using mouse and human samples.…”

Section: Discussionmentioning

confidence: 99%

Implementation of a red blood cell-optical (RBO) channel for detection of latent iron deficiency anaemia by automated measurement of autofluorescence-emitting red blood cells

Tougan

Itagaki

Toya

et al. 2020

Sci Rep

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Iron deficiency is the most common and widespread nutritional disorder worldwide. The automated haematology analyser XN-30 (Sysmex, Kobe, Japan) was developed to detect malaria-infected red blood cells (RBCs) in human blood samples using flow cytometry. The optical system of the analyser detects autofluorescence (AF)-emitting RBCs containing iron-deficient haem groups and would aid in the diagnosis of anaemia resulting from iron deficiency. Here, an RBC-optical (RBO) channel was devised and implemented on the analyser. In vitro analyses showed that the analyser detected AF-emitting RBCs treated with 5-aminolevulinic acid. Furthermore, the analyser detected AF-emitting RBCs in mice fed a low iron diet and infected with a rodent malaria parasite; it could also be effectively used in humans. This study demonstrates that the analyser can quantitatively and reproducibly detect AF-emitting RBCs and measure other haematological parameters, suggesting its usefulness for the initial evaluation of latent iron deficiency anaemia in conjunction with the diagnosis of malaria.

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“…Random-walk models can accurately predict the volume of tissue in which neurons are activated or silenced and have been used to refine stimulation parameters to minimize heating ( Bernstein et al, 2008 ; Kahn et al, 2011 ; Liu et al, 2015 ; Stujenske et al, 2015 ; Yona et al, 2016 ). Fluorescence has been simulated by separating illumination and the fluorescence detection into two processes ( Chen et al, 2012 ; Holt et al, 2015 ; Hennig et al, 2016 ) and widefield fluorescence has been studied for columnar arrangements of fluorophores, revealing that the effects of numerical aperture and focal position within the tissue depend on the size of the column ( Tian et al, 2011 ).…”

Section: Introductionmentioning

confidence: 99%

Sources of widefield fluorescence from the brain

Waters

2020

eLife

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Widefield fluorescence microscopy is used to monitor the spiking of populations of neurons in the brain. Widefield fluorescence can originate from indicator molecules at all depths in cortex and the relative contributions from somata, dendrites, and axons are often unknown. Here, I simulate widefield illumination and fluorescence collection and determine the main sources of fluorescence for several GCaMP mouse lines. Scattering strongly affects illumination and collection. One consequence is that illumination intensity is greatest ~300–400 µm below the pia, not at the brain surface. Another is that fluorescence from a source deep in cortex may extend across a diameter of 3–4 mm at the brain surface, severely limiting lateral resolution. In many mouse lines, the volume of tissue contributing to fluorescence extends through the full depth of cortex and fluorescence at most surface locations is a weighted average across multiple cortical columns and often more than one cortical area.

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Non-invasive detection of iron deficiency by fluorescence measurement of erythrocyte zinc protoporphyrin in the lip

Cited by 41 publications

References 39 publications

The management of anaemia and haematinic deficiencies in pregnancy and post‐partum

The management of anaemia and haematinic deficiencies in pregnancy and post‐partum

Implementation of a red blood cell-optical (RBO) channel for detection of latent iron deficiency anaemia by automated measurement of autofluorescence-emitting red blood cells

Sources of widefield fluorescence from the brain

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