Non-Invasive Diagnosis of Helicobacter pylori: Evaluation of Two Enzyme Immunoassays, Testing Serum IgG and IgA Response in the Anand District of Central Gujarat, India

Pandya, Himani; Patel, Jagdish; Agravat, Harihar; Singh, Navneet kumar Ramdayal

doi:10.7860/jcdr/2014/7578.4480

Cited by 11 publications

(12 citation statements)

References 19 publications

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“…Compared to the stool Ag test, a sensitivity, specificity and agreement of 98.18, 97.78 and 98%, respectively were recorded for serum IgG Ab test (Table 2) These results were in accordance with the findings of Pandya et al, (Pandya et al, 2014), who reported that the IgG Ab test evaluated in their study, had a sensitivity of 100%, which permits the safe use of this test in screening surveys. Unlikely, Shah et al, (Shah et al, 2014), reported that serum IgG sensitivity ranged from 90 to 97%, but the specificity ranged from 50 to 96%.…”

Section: Resultssupporting

confidence: 89%

Screening for Helicobacter pylori Infection among Asymptomatic University Students in Alexandria, Egypt, Using Non Invasive Laboratory Techniques

dir¹,

Kady²

2018

Int.J.Curr.Microbiol.App.Sci

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Section: Resultssupporting

confidence: 89%

Screening for Helicobacter pylori Infection among Asymptomatic University Students in Alexandria, Egypt, Using Non Invasive Laboratory Techniques

dir¹,

Kady²

2018

Int.J.Curr.Microbiol.App.Sci

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“…It was found that serum IgG Ab was positive in 19 of the 21 enrolled mothers (90%) with sensitivity, specificity and agreement of 94, 50 and 90%, respectively) ( Table 6). These results were in concordance with the findings of Pandya et al (2014) who reported that the IgG Abs evaluated in their study, had a sensitivity of 100% in adults, which permits the safe use of the test in epidemiologic surveys. Also, they recorded a very low specificity, indicating that the ELISA test must be validated for different populations (Pandya et al, 2014).…”

Section: Discussionsupporting

confidence: 93%

“…These results were in concordance with the findings of Pandya et al (2014) who reported that the IgG Abs evaluated in their study, had a sensitivity of 100% in adults, which permits the safe use of the test in epidemiologic surveys. Also, they recorded a very low specificity, indicating that the ELISA test must be validated for different populations (Pandya et al, 2014). In addition, Shah et al (2014) reported that serum IgG sensitivity ranged between 90 and 97%, but the specificity ranged between 50 and 96%.…”

Section: Discussionsupporting

confidence: 93%

Diagnosis of Helicobacter pylori infection in children and their mothers using some non-invasive techniques

Mohamed¹,

Zalabany²,

Abaza³

et al. 2016

Afr. J. Microbiol. Res.

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Helicobacter pylori (H. pylori) is an important global public health concern. It infects approximately one half of the world's population. Considering the broad spectrum of available H. pylori diagnostic methods and since a reliable diagnosis is mandatory both before and after eradication therapy; thus it is crucial to study the different methods to be able to select the highly accurate laboratory tests that could be used to efficiently diagnose H. pylori infection in clinical practice. In this descriptive-cross sectional study we aimed to evaluate some non-invasive techniques for the diagnosis of H. pylori infection in children and their mothers. Forty children and their 21 mothers who had gastrointestinal symptoms were included. Stool, serum and saliva samples were collected from each enrolled child and mother to detect H. pylori antigens (Ags) in stool using chromatographic immunoassay and IgG antibodies (Abs) in serum and saliva by enzyme linked immune sorbent assay (ELISA). Collected data were analyzed using IBM SPSS software package version. Thirty (75%) children were positive for stool Ags, 10 (25%) were positive for serum Abs and only 5.4% were positive for saliva Abs. H. pylori stool Ag test was the most reliable test in all studied individuals and testing H. pylori IgG Abs in serum was more useful in adults than in children.

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“…In this study, (88.8%) have H. pylori -specific IgG antibodies (˃ 30-EIU) which reflect the high level of immune response to H. pylori as the mean of H. pylori -specific IgG antibodies (107.61 ± 52) EIU, which come in agreement with [ 20 ] and higher than [ 21 ] [ 22 ], reporting H. pylori seropositivity of 56.3%; 57% of Indian and Saudi Arabia patients respectively. The negative H. pylori -specific IgG (˂ 30-EIU) was detected in (11.3%) but, histologically the infection proved through detection of Cag A gene expression in gastric tissue.…”

Section: Discussionsupporting

confidence: 83%

“…H. pylori infection provokes both local and systemic antibody responses. The systemic response typically comprises a transient rise in IgM, followed by a rise in specific IgA and IgG maintained throughout infection [ 20 ].…”

Section: Discussionmentioning

confidence: 99%

Immunopathological and Modulatory Effects of Cag A+ Genotype on Gastric Mucosa, Inflammatory Response, Pepsinogens, and Gastrin-17 Secretion in Iraqi Patients infected with H. pylori

Al-Ezzy¹

2018

Open Access Maced J Med Sci

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OBJECTIVE:To determine the immunopathological correlation between Cag A+ H. pylori-specific IgG; pepsinogen I&II (PI&PII); gastrin-17 (G-17); status of gastric and duodenal mucosa and inflammatory activities on different gastroduodenal disorders.METHODOLOGY:Eighty gastroduodenal biopsies were taken from patients with gastroduodenal disorders for histopathological evaluation and H. pylori diagnosis. Serum samples were used for evaluation of gastric hormones and detection of H. pylori-specific IgG antibodies. The tissue expression of H. pylori Cag A gene was detected by in situ hybridisation.RESULTS:H. pylori IgG antibodies were detected in (88.8%) of enrolled patients. According to Cag A gene expression, Significant difference (P value ˂ 0.05) was detected in levels of PG I; PGII, PG I/PG II among patients with gastric disorders. Serum G-17 level was negatively correlated with Cag A gene expression (P-value = 0.04). There was a significant correlation between H. pylori IgG and PG I; PG II; G-17. The current study revealed that corpus atrophic gastritis was diagnosed histologically with (5%) gastric ulcer cases; (3.75%) of duodenal ulcer cases; (3.75%) of duodenitis cases; (1.25%) of gastropathy cases and (8.75%) of gastritis cases. At the same time H. pylori gastritis diagnosed concurrently with (8.75%) of gastric ulcer cases; (11.25%) of duodenal ulcer cases; (17.5%) of gastropathy cases; (3.75%) of duodenitis cases and (2.5%) of prepyloric ulcer cases. A significant correlation was reported between the Immunopathological status of gastric mucosa and endoscopic mucosal finding among duodenal ulcer cases and gastritis cases only. A positive correlation was reported between serum levels of PGI; PGII; PGI/PGII; G-17; PMNs grade and Immunopathological status of the gastroduodenal mucosa of H. pylori Infected patients. A significant difference was reported in lymphocyte grades among gastric disorders without correlation with immunohistopathological changes in the mucosa (P-value = 0.002). A significant difference was reported in lymphocyte grades among different disorders according to H. pylori IgG. A significant difference was reported in serum level of PG I; PG II; PG I/PG II; G-17 according to PMN and lymphocyte grades (P-value ˂ 0.01). PMNs grades positively correlated with gastric Cag A expression; H. pylori IgG; PG II; G-17 levels. PG I; PG I/ PG II correlated with lymphocyte grades (P-value ˂ 0.05); while PGII has a negative correlation (P-value = 0.039).CONCLUSION:Endoscopic mucosal finding does not reflect exactly the actual immunopathological changes of gastric mucosa during H. pylori infection. Secretion of gastrin was not affected by the presence of Cag A in gastric tissue. Instead, the fluctuation in the hormone level appears to be due to the presence of H. pylori infection in gastric tissue. Gastric tissue infiltration with PMNs & lymphocytes inflammatory infiltrates has a direct effect on PGs and gastrin levels in serum of infected patients. The level of PG I; PG II; G-17 secretion correlated with the d...

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Non-Invasive Diagnosis of Helicobacter pylori: Evaluation of Two Enzyme Immunoassays, Testing Serum IgG and IgA Response in the Anand District of Central Gujarat, India

Abstract: Context: Validation of an accurate and less cumbersome noninvasive method to detect current Helicobacter pylori infection is a requisite for any laboratory.

Cited by 11 publications

References 19 publications

Screening for Helicobacter pylori Infection among Asymptomatic University Students in Alexandria, Egypt, Using Non Invasive Laboratory Techniques

Screening for Helicobacter pylori Infection among Asymptomatic University Students in Alexandria, Egypt, Using Non Invasive Laboratory Techniques

Diagnosis of Helicobacter pylori infection in children and their mothers using some non-invasive techniques

Immunopathological and Modulatory Effects of Cag A+ Genotype on Gastric Mucosa, Inflammatory Response, Pepsinogens, and Gastrin-17 Secretion in Iraqi Patients infected with H. pylori

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