Non-invasive diagnostic and monitoring of periodontal disease through optical coherence tomography: validation of the technique with animal model and patients

Mota, Cláudia Cristina Brainer de Oliveira; Fernandes, Luana Osório; Neves, José Kayque; Oliveira, Hugo O.; Melo, Letícia Santos Alves de; Dias, Tereza Januária Costa; Pires, Natalia S. M.; Santiago, Leógenes Maia; Gomes, Anderson S. L.

doi:10.1364/cleo_at.2017.jw2a.50

Cited by 1 publication

(2 citation statements)

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“…Other studies have attempted to characterize periodontal tissues through unilateral modalities. [22][23][24] Chifor et al concluded that periodontal ultrasonography can accurately visualize the dentino-enamel junction, cortical bone, and gingival tissues to diagnose periodontal status. 25 Similarly, Tattan et al reports that ultrasonic imaging can provide accurate periodontal diagnosis without concerns about ionizing radiation when compared with clinical and CBCT readings.…”

Section: Discussionmentioning

confidence: 99%

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Raman microspectroscopy/micro‐optical coherence tomography approach for chairside diagnosis of periodontal diseases: A pilot study

Saggu

Maguluri

Grimble

et al. 2022

Journal of Periodontology

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Background Our objective was to develop and test a combined Raman microspectroscopy (RMS) and micro‐optical coherence tomography (μOCT) approach for chairside quantification of gingival collagen, DNA, epithelium, and connective tissue. We hypothesized that a high‐resolution RMS/μOCT can characterize healthy and inflamed periodontal tissues for diagnosis and disease activity monitoring. Methods A prototype instrument was developed, tested ex vivo on gingival specimens and optimized for in vivo intraoral use. The primary outcome measures were the ratios of oral epithelium to connective tissue thickness (OE:CT) and the amount of DNA to collagen type I (DNA/Col 1), and the thickness of sulcular epithelium (SE). For ex vivo testing, eight subjects with healthy periodontal tissues or with Stage II to IV periodontitis were included in the study and underwent crown‐lengthening or periodontal surgical procedures, respectively. Gingival biopsies were scanned by RMS/μOCT and histometric analyses were performed. The proof‐of‐concept study included OE/CT, DNA/Col 1, and SE assessed in six volunteers with or without signs of gingival inflammation (n = 3/group). Results The spatially co‐registered RMS spectra revealed opposing changes in the collagen and DNA peaks of inflamed compared with healthy tissues (P <0.05). Combined RMS/μOCT analysis showed that OE/CT, DNA/Col, and SE are significantly different between healthy and inflamed sites (P <0.05). Histological assessments confirmed the differences detected by RMS/μOCT. Qualitative analysis of DNA/Col 1 ratios indicated Col I content as the main distinguishing feature for health and DNA content for periodontitis. Conclusion Results suggest that combined RMS/μOCT chairside imaging may distinguish between healthy and diseased sites by evaluating marginal periodontal morphological and biochemical features.

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Section: Discussionmentioning

confidence: 99%

“…To the best of the authors’ knowledge, this is the first study to combine RMS and μOCT into a single optical device for measurement of periodontal activity status. Other studies have attempted to characterize periodontal tissues through unilateral modalities 22–24 . Chifor et al.…”

Section: Discussionmentioning

confidence: 99%