Non-invasive <i>in vivo</i> molecular imaging of intra-articularly transplanted immortalized bone marrow stem cells for osteoarthritis treatment

Ningrum

et al. 2018

IJMS

Self Cite

Recent years have witnessed an increased prevalence of knee osteoarthritis (KOA) among diabetes mellitus (DM) patients—conditions which might share common risk factors such as obesity and advanced aging. Therefore, we conducted dry-to-wet lab research approaches to assess the correlation of type 1 DM (T1DM) and type 2 DM (T2DM) with KOA among all age and genders of Taiwanese population. The strength of association (odds ratio: OR) was analyzed using a phenome-wide association study portal. Populations of 37,353 T1DM and 1,218,254 T2DM were included. We observed a significant association of KOA with T1DM (OR: 1.40 (1.33–1.47), p< 0.0001) and T2DM (OR: 2.75 (2.72–2.78), p< 0.0001). The association between T1DM and KOA among the obese (OR: 0.99 (0.54–1.67), p = 0.0477) was insignificant compared to the non-obese (OR: 1.40 (1.33–1.48), p < 0.0001). Interestingly, a higher association between T2DM and KOA among non-obese persons (OR: 2.75, (2.72–2.79), p < 0.0001) compared to the obese (OR: 1.71 (1.55–1.89), p < 0.0001) was noted. Further, histopathologic and Western blot studies of diabetic mice knee joints revealed enhanced carboxymethyl lysine (advanced glycation end product), matrix metalloproteinase-1, and reduced cartilage-specific proteins, including type II collagen (Col II), SOX9, and aggrecan (AGN), indicating deteriorated articular cartilage and proteoglycans. Results indicate that DM is strongly associated with KOA, and obesity may not be a confounding factor.

Section: Discussionmentioning

confidence: 99%

Correlation between Diabetes Mellitus and Knee Osteoarthritis: A Dry-To-Wet Lab Approach

Ningrum

et al. 2018

IJMS

Self Cite

Osteoarthritis and Cartilage

“…Luciferase reporters can also be used in transplanted cell tracking experiments using the same optical imaging systems to demonstrate the presence of exogenous cells in repairing tissues of OA rats following ACLT 55,56 and persistence of transplanted senescent auricular chondrocytes when injected into mouse joints to cause OA like disease 57 .…”

Section: Optical Imaging -Bioluminescencementioning

confidence: 99%

Molecular and structural imaging in surgically induced murine osteoarthritis

Lim

Wen

Vincent

2020

Preclinical imaging in osteoarthritis is a rapidly growing area with three principal objectives: to provide rapid, sensitive tools to monitor the course of experimental OA longitudinally; to describe the temporal relationship between tissue-specific pathologies over the course of disease; and to use molecular probes to measure disease activity in vivo. Research in this area can be broadly divided into those techniques that monitor structural changes in tissues (microCT, microMRI, ultrasound) and those that detect molecular disease activity (positron emission tomography (PET), optical and optoacoustic imaging). The former techniques have largely evolved from experience in human joint imaging and have been refined for small animal use. Some of the latter tools, such as optical imaging, have been developed in preclinical models and may have translational benefit in the future for patient stratification and for monitoring disease progression and response to treatment. In this narrative review we describe these methodologies and discuss the benefits to animal research, understanding OA pathogenesis, and in the development of human biomarkers.

“…MSCs are capable to differentiate into osteoblasts and also secrete factors such as VEGF and BMP-4 to promote bone cell regeneration [ 167 , 168 ]. Studies have also used immortalized BMSCs in osteoarthritic recovery [ 169 ]. These studies showed the potential of MSC therapy in bone-associated disorders.…”

Section: Stem Cells In Macrovascular and Other Complicationsmentioning

confidence: 99%

Addressing Stem Cell Therapeutic Approaches in Pathobiology of Diabetes and Its Complications

Peng

Journal of Diabetes Research

Mishra³

et al. 2018

Self Cite

High morbidity and mortality of diabetes mellitus (DM) throughout the human population is a serious threat which needs to be addressed cautiously. Type 1 diabetes mellitus (T1DM) and type 2 diabetes mellitus (T2DM) are most prevalent forms. Disruption in insulin regulation and resistance leads to increased formation and accumulation of advanced end products (AGEs), which further enhance oxidative and nitrosative stress leading to microvascular (retinopathy, neuropathy, and nephropathy) and macrovascular complications. These complications affect the normal function of organ and tissues and may cause life-threatening disorders, if hyperglycemia persists and improperly controlled. Current and traditional treatment procedures are only focused on to regulate the insulin level and do not cure the diabetic complications. Pancreatic transplantation seemed a viable alternative; however, it is limited due to lack of donors. Cell-based therapy such as stem cells is considered as a promising therapeutic agent against DM and diabetic complications owing to their multilineage differentiation and regeneration potential. Previous studies have demonstrated the various impacts of both pluripotent and multipotent stem cells on DM and its micro- and macrovascular complications. Therefore, this review summarizes the potential of stem cells to treat DM and its related complications.