Non invasive jacketed telemetry in socially-housed rats for a combined assessment of respiratory system, electrocardiogram and activity using the DECRO system

Fares, Raafat; Flénet, Timothé; Vial, Jonathan; Ravaz, Marine; Roger, Virginie; Bory, Christophe; Baudet, Stéphane

doi:10.1016/j.vascn.2022.107195

Cited by 7 publications

(6 citation statements)

References 27 publications

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“…Although not all parameters measured by telemetry can be analyzed using beam-based tracking and visual object tracking, activity can be monitored using non-invasive methods. Moreover, non-invasive alternatives such as jacketed telemetry could be applied for assessing respiration, collecting an electrocardiogram, or measuring activity [ 106 ].…”

Section: Discussionmentioning

confidence: 99%

A systematic review of the development and application of home cage monitoring in laboratory mice and rats

Kahnau,

Mieske,

Wilzopolski

et al. 2023

BMC Biol

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Background Traditionally, in biomedical animal research, laboratory rodents are individually examined in test apparatuses outside of their home cages at selected time points. However, the outcome of such tests can be influenced by various factors and valuable information may be missed when the animals are only monitored for short periods. These issues can be overcome by longitudinally monitoring mice and rats in their home cages. To shed light on the development of home cage monitoring (HCM) and the current state-of-the-art, a systematic review was carried out on 521 publications retrieved through PubMed and Web of Science. Results Both the absolute (~ × 26) and relative (~ × 7) number of HCM-related publications increased from 1974 to 2020. There was a clear bias towards males and individually housed animals, but during the past decade (2011–2020), an increasing number of studies used both sexes and group housing. In most studies, animals were kept for short (up to 4 weeks) time periods in the HCM systems; intermediate time periods (4–12 weeks) increased in frequency in the years between 2011 and 2020. Before the 2000s, HCM techniques were predominantly applied for less than 12 h, while 24-h measurements have been more frequent since the 2000s. The systematic review demonstrated that manual monitoring is decreasing in relation to automatic techniques but still relevant. Until (and including) the 1990s, most techniques were applied manually but have been progressively replaced by automation since the 2000s. Independent of the year of publication, the main behavioral parameters measured were locomotor activity, feeding, and social behaviors; the main physiological parameters were heart rate and electrocardiography. External appearance-related parameters were rarely examined in the home cages. Due to technological progress and application of artificial intelligence, more refined and detailed behavioral parameters have been investigated in the home cage more recently. Conclusions Over the period covered in this study, techniques for HCM of mice and rats have improved considerably. This development is ongoing and further progress as well as validation of HCM systems will extend the applications to allow for continuous, longitudinal, non-invasive monitoring of an increasing range of parameters in group-housed small rodents in their home cages.

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Section: Discussionmentioning

confidence: 99%

A systematic review of the development and application of home cage monitoring in laboratory mice and rats

Kahnau,

Mieske,

Wilzopolski

et al. 2023

BMC Biol

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“…Additionally, all of these systems are most likely to be used during people’s normal working hours (which is the resting phase of mice) and acquisition during night (the active phase of mice) is more challenging. Newly developed non-invasive telemetry jackets are available but so far only for larger animals, rats and hamsters (Fares et al 2022 ; Flenet et al 2020 ).…”

Section: Non-invasive Methods For Ecg Acquisitionmentioning

confidence: 99%

What to consider for ECG in mice—with special emphasis on telemetry

Calvet

Seebeck

2023

Mamm Genome

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Genetically or surgically altered mice are commonly used as models of human cardiovascular diseases. Electrocardiography (ECG) is the gold standard to assess cardiac electrophysiology as well as to identify cardiac phenotypes and responses to pharmacological and surgical interventions. A variety of methods are used for mouse ECG acquisition under diverse conditions, making it difficult to compare different results. Non-invasive techniques allow only short-term data acquisition and are prone to stress or anesthesia related changes in cardiac activity. Telemetry offers continuous long-term acquisition of ECG data in conscious freely moving mice in their home cage environment. Additionally, it allows acquiring data 24/7 during different activities, can be combined with different challenges and most telemetry systems collect additional physiological parameters simultaneously. However, telemetry transmitters require surgical implantation, the equipment for data acquisition is relatively expensive and analysis of the vast number of ECG data is challenging and time-consuming. This review highlights the limits of non-invasive methods with respect to telemetry. In particular, primary screening using non-invasive methods can give a first hint; however, subtle cardiac phenotypes might be masked or compensated due to anesthesia and stress during these procedures. In addition, we detail the key differences between the mouse and human ECG. It is crucial to consider these differences when analyzing ECG data in order to properly translate the insights gained from murine models to human conditions.

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Section: Use Of Novel Techniques Allows For Monitoring Sophisticated ...mentioning

confidence: 99%

Development and Application of Home Cage Monitoring in Laboratory Mice and Rats: a Systematic Review

Kahnau

Mieske

Wilzopolski

et al. 2023

Preprint

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Traditionally, in biomedical animal research, laboratory rodents are individually examined in test apparatuses outside their home cages at selected time points. However, the outcome of such tests can be influenced by the novel environment, the time of day, separation from the social group, or the presence of an experimenter. Moreover, valuable information may be missed when the animals are only monitored in short periods. These issues can be overcome by longitudinal monitoring mice and rats in their home cages. To shed light on the development of home cage monitoring (HCM) and the current state of the art, a systematic review was carried out on 521 publications retrieved through PubMed and Web of Science. Both the absolute (~ x26) and relative (~ x7) number of HCM-related publications increased from 1974 to 2020. In both mice and rats, there was a clear bias towards males and individually housed animals, but during the past decade (2011-2020), an increasing number of studies used both sexes and group housing. More than 70% of the studies did not involve a disease model, but the percentage of studies using disease models increased since the 2000s. In most studies, animals were kept for short (up to 4 weeks) length periods in the HCM systems; intermediate length periods (4-12 weeks) increased in frequency in the years between 2011 and 2020. Before the 2000s, HCM techniques were predominantly applied for less than 12 hours, while 24-hour measurements have been more frequently since the 2000s. The systematic review demonstrated that manual monitoring is decreasing but still relevant. Until (and including) the 1990s, most techniques were applied manually but have been progressively replaced by automation since the 2000s. Independent of the publication year, the main behavioral parameters measured were locomotor activity, feeding, and social behaviors; the main physiological parameters were heart rate and electrocardiography. External appearance-related parameters were rarely examined in the home cages. Due to technological progress and application of artificial intelligence, more refined and detailed behavioral parameters could be investigated in the home cage in recent times. Over the period covered in this study, techniques for HCM of mice and rats has improved considerably. This development is ongoing and further progress and validation of HCM systems will extend the applications to allow for continuous, longitudinal, non-invasive monitoring of an increasing range of parameters in group-housed small rodents in their home cages.

show abstract

Non invasive jacketed telemetry in socially-housed rats for a combined assessment of respiratory system, electrocardiogram and activity using the DECRO system

Cited by 7 publications

References 27 publications

A systematic review of the development and application of home cage monitoring in laboratory mice and rats

A systematic review of the development and application of home cage monitoring in laboratory mice and rats

What to consider for ECG in mice—with special emphasis on telemetry

Development and Application of Home Cage Monitoring in Laboratory Mice and Rats: a Systematic Review

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