Non-invasive measurement of cell membrane associated proton gradients by ion-sensitive field effect transistor arrays for microphysiological and bioelectronical applications

Lehmann, Mirko; Baumann, Werner; Brischwein, M.; Ehret, Ralf; Kraus, Michael; Schwinde, Anne; Bitzenhofer, Matthias; Freund, Ingo; Wolf, Bernhard

doi:10.1016/s0956-5663(00)00065-8

Cited by 98 publications

(69 citation statements)

References 17 publications

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“…After that it becomes saturated for both the normal cells and the NSCLC cells. The ΔI saturation indicates that 24 h is needed for a cell to completely adhere to the PSW surface, which is consistent with the data reported in (Baumann et al 1999) (Lehmann et al 2000) (Otto et al 2003) (Cai et al 2009). The other item we observed from Fig.…”

Section: Single Cell Characterizationsupporting

confidence: 91%

“…Generally, it takes 1~2 days for a cell to completely adhere to a substrate (Baumann et al 1999) (Lehmann et al 2000) (Otto et al 2003) (Yu et al 2009). We therefore measured the current changes ΔI of the PSW at V DS =5V for the single normal cells and the NSCLC cells from the very beginning of seeding the single cell into the isolation window as a function of time.…”

Section: Single Cell Characterizationmentioning

confidence: 99%

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Electrical characterization of single cells using polysilicon wire ion sensor in an isolation window

Hsu

et al. 2011

Biomed Microdevices

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A polysilicon wire (PSW) sensor can detect the H(+) ion density (pH value) of the medium coated on its surface, and different cells produce different extracellular acidification and hence different H(+) ion densities. Based on this, we used a PSW sensor in combination with a mold-cast polydimethylsiloxane (PDMS) isolation window to detect the adhesion, apoptosis and extracellular acidification of single normal cells and single cancer cells. Single living human normal cells WI38, MRC5, and BEAS-2B as well as non-small-cell lung cancer (NSCLC) cells A549, H1299, and CH27 were cultivated separately inside the isolation window. The current flowing through the PSW channel was measured. From the PSW channel current change as a function of time, we determined the cell adhesion time by observing the time required for the current change to saturate, since a stable extracellular ion density was established after the cells were completely adhered to the PSW surface. The apoptosis of cells can also be determined when the channel current change drops to zero. We found that all the NSCLC cells had a higher channel current change and hence a lower pH value than the normal cells anytime after they were seeded. The corresponding average pH values were 5.86 for A549, 6.00 for H1299, 6.20 for CH27, 6.90 for BEAS-2B, 6.96for MRC5, and 7.02 for WI38, respectively, after the cells were completely adhered to the PSW surface. Our results show that NSCLC cells have a stronger cell-substrate adhesion and a higher extracellular acidification rate than normal cells.

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Section: Single Cell Characterizationsupporting

confidence: 91%

Section: Single Cell Characterizationmentioning

confidence: 99%

Electrical characterization of single cells using polysilicon wire ion sensor in an isolation window

Hsu

et al. 2011

Biomed Microdevices

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“…The sensor chip enables continuous measurement of oxygen consumption using oxygen-sensitive electrodes [27], pH changes of the medium by employing ionsensitive field-effect transistors [28], and the impedance between two interdigitated electrode structures [29] to register the impedance under and across the cell layer on the chip surface. Before measurement, cells were seeded on the sensor chip (SC 1000) in DMEM (PAA, E15-883) with penicillin/streptomycin and 10% (v/v) FCS (PAA) and incubated in a standard tissue culture incubator at 37°C/5% CO 2 and 95% humidity for 24 h until 80-90% confluency was reached.…”

Section: Cellular Metabolismmentioning

confidence: 99%

“…The impact of complexes 1 and 2 and 11 and 12 on the metabolism of adherent MCF-7 cells was monitored using a Bionas biosensor chip system, whose silicon chip contains two interdigitated electrode structures that register the cellular impedance [29], miniature Clarke-type oxygen electrodes for recording the cellular oxygen uptake [27], and ion-sensitive field-effect transistors to monitor pH changes of the medium [28]. As the cells were plated at high density in the Bionas system, a much lower proliferation rate was observed than in the crystal violet assay, where MCF-7 cell duplication took about 30 h. The impedance value reflects the integrity of the cellular layer but not the total number of cells and its decrease therefore provides a measure of the extent of cell death rather than the level of antiproliferative activity.…”

Section: Metabolism Of Mcf-7 Cellsmentioning

confidence: 99%

Cellular impact and selectivity of half-sandwich organorhodium(III) anticancer complexes and their organoiridium(III) and trichloridorhodium(III) counterparts

et al. 2012

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Half-sandwich organorhodium(III) complexes and their trichloridorhodium(III) counterparts are potent anticancer agents that enhance the formation of reactive oxygen species and invoke a strong induction of apoptosis in leukemia cells. The antiproliferative activity towards human MCF-7 and HT-29 adenocarcinoma cells of novel nonintercalating complexes containing the 5-substituted phenanthroline ligands 5,6-dimethylphenanthroline, 5-chlorophenanthroline, and 5-nitrophenanthroline (phen*) increases dramatically in the order [(η(5)-C(5)Me(5))IrCl(phen*)](CF(3)SO(3)) < [(η(5)-C(5)Me(5))RhCl(phen*)](CF(3)SO(3)) < mer-[RhCl(3)(DMSO)(phen*)] (DMSO is dimethyl sulfoxide). Improved activity was also achieved by attaching a cell-penetrating peptide to the dipyrido[3,2-a:2',3'-c]phenazine (dppz) ligand of an organorhodium(III) complex. Whereas 5-substitution led to significant improvements in the activity of the organoiridium(III) and trichloridorhodium(III) compounds in comparison with the parent phenanthroline complex, the IC(50) values of their organorhodium(III) counterparts remained effectively invariable. The high activities of the trichloridorhodium(III) complexes (IC(50) = 0.06-0.13 μM) were accompanied by pronounced selectivity towards human cancer cells in comparison with immortalized HEK-293 cells. In contrast, [(η(5)-C(5)Me(5))RhCl(5,6-Me(2)phen)](CF(3)SO(3)) (phen is phenanthroline) was markedly more active towards BJAB lymphoma cells than ex vivo healthy leukocytes and caused an immediate decrease in cellular adhesion possibly associated with interactions with membrane proteins. Its dppz analogue invoked an initial increase in glycolysis to compensate for reduced respiration before inducing a delayed onset of cell death. Strong antimitochondrial activity with respiration impairment and release of cytochrome c was established for both complexes.

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“…It is illuminating to contrast the previous application with another physiological application of pH ISFET, in which changes of local pH have been measured with a wellpassivated transistor [9]. In this case the signal provided true chemical information.…”

Section: Introductionmentioning

confidence: 99%

Thirty Years of CHEMFETs – A Personal View

Janata

2004

Electroanalysis

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Non-invasive measurement of cell membrane associated proton gradients by ion-sensitive field effect transistor arrays for microphysiological and bioelectronical applications

Cited by 98 publications

References 17 publications

Electrical characterization of single cells using polysilicon wire ion sensor in an isolation window

Electrical characterization of single cells using polysilicon wire ion sensor in an isolation window

Cellular impact and selectivity of half-sandwich organorhodium(III) anticancer complexes and their organoiridium(III) and trichloridorhodium(III) counterparts

Thirty Years of CHEMFETs – A Personal View

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