Non-Invasive Measurement of Skin Microvascular Response during Pharmacological and Physiological Provocations

Iredahl, Fredrik; Löfberg, Andreas; Sjöberg, Folke; Farnebo, Simon; Tesselaar, Erik

doi:10.1371/journal.pone.0133760

Cited by 57 publications

(45 citation statements)

References 44 publications

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“…Skin perfusion is estimated based on the reduction of the local speckle contrast in the reflected image. The technique has previously been described in detail . Perfusion images were acquired by averaging data from 42 images taken in rapid succession (acquisition time 2 seconds) and then further analyzed by calculating mean perfusion levels in regions of interest (ROI) using PIMsoft 1.3 (Perimed AB, Järfälla, Sweden).…”

Section: Methodsmentioning

confidence: 99%

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Skin glucose metabolism and microvascular blood flow during local insulin delivery and after an oral glucose load

et al. 2016

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Skin glucose metabolism and microvascular blood flow during local insulin delivery and after an oral glucose load, Microcirculation, 2016. 23 (7) Grants:The study has been financially supported by ALF grants, Region Östergötland, by Sinnescentrum, Region Östergötland, and by The Grönberg Foundation. Keywords:Insulin, Skin, Metabolism, Microcirculation, Microdialysis AbstractInsulin causes capillary recruitment in muscle and adipose tissue, but the metabolic and microvascular effects of insulin in the skin have not been studied in detail. The aim of this study was to measure glucose metabolism and microvascular blood flow in the skin during local insulin delivery and after an oral glucose load using intracutaneous microdialysis and laser speckle contrast imaging (LSCI).Within 15 minutes of local insulin delivery, microvascular blood flow in the skin increased (urea clearance: p=0.047, LSCI: p=0.002) paralleled by increases in pyruvate (p=0.01) and lactate (p=0.04), indicating an increase in glucose uptake. Thus, local delivery of insulin to the skin via microdialysis resulted in rapid vasodilatation, paralleled by an increased glucose uptake. An oral glucose load increased urea clearance from the catheters, indicating an increase in skin perfusion, although no perfusion changes were detected with LSCI. The time course of the effects of insulin on glucose uptake in the skin differed markedly depending on whether insulin is delivered directly to the interstitium via microdialysis, or via a systemic route. These results show that insulin has specific metabolic and vasodilatory effects in the skin both when given locally and after systemic delivery through an oral glucose load.

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Section: Methodsmentioning

confidence: 99%

“…The technique has previously been described in detail. 24,25 Perfusion images were acquired by averaging data from 42 images taken in rapid succession…”

Section: Study Protocolmentioning

confidence: 99%

Skin glucose metabolism and microvascular blood flow during local insulin delivery and after an oral glucose load

et al. 2016

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“…Laser Doppler flowmetry (LDF) and laser Doppler perfusion imaging (LDPI) are commonly used, non‐invasive methods to measure skin microcirculation . Another established technology for assessing skin microcirculation is Tissue Viability (TiVi) . Laser speckle contrast imaging (LSCI) is a relatively new promising non‐contact technology for assessing microcirculation of the skin .…”

Section: Introductionmentioning

confidence: 99%

Perfusion of the skin’s microcirculation after cold‐water immersion (10°C) and partial‐body cryotherapy (−135°C)

Hohenauer

Deliens

Clarys

et al. 2019

Skin Research and Technology

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BackgroundInvestigations of the perfusion of the skin's microcirculation with laser speckle contrast imaging (LSCI) after cold treatments are rare. Therefore, the aim of this study was to compare the effects between cold‐water immersion (CWI) conduction and partial‐body cryotherapy (PBC) convection on perfusion of the microcirculation and skin temperature on the thigh.Materials and MethodsTwenty healthy males were randomly allocated to CWI (10°C for 10 minutes) or PBC (−60°C for 30 seconds, −135°C for 2 minutes). Perfusion and skin temperature measurements were conducted on the anterior thigh region up to 60 minutes post‐treatment.ResultsCold‐water immersion decreased perfusion of the microcirculation significantly compared to baseline values between 10 minutes (P = 0.003) and 30 minutes (P = 0.01) post‐treatment. PBC increased perfusion of the microcirculation and decreased skin temperature only at the first measurement interval (0 minute, both P = 0.01) post‐treatment. Additionally, local skin temperature was significantly decreased compared to baseline values only after CWI up to 30 minutes (P = 0.04) post‐treatment.ConclusionCold‐water immersion reduced local skin microcirculation and skin temperature while PBC only slightly increased the perfusion of the microcirculation immediately after the treatment. For cooling purposes, the conduction method seems superior compared to the convection method, assessed with a LSCI device.

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“…Such a control would allow improvement of repeatability and fidelity of the measured data. Moreover, further research, which for example may include the study of BPA response on the reactive hyperemia with a local heating after occlusion of the brachial artery [32,33], is needed to determine the range of the BPA dynamics in terms of biological zero and maximal blood flow. Study of the dynamics of the PPG waveform in these conditions is an important step for development of reliable indicators of the cutaneous blood flow and its variability in changeable physiological and pathological conditions.…”

Section: Discussionmentioning

confidence: 99%

Local Thermal Impact on Microcirculation Assessed by Imaging Photoplethysmography

Kamshilin¹,

Belaventseva²,

Romashko³

et al. 2016

Biol Med (Aligarh)

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Laser Doppler Flowmetry and Laser Speckle Contrast Imaging are applied usually for assessment of parameters of the cutaneous blood flow during thermoregulation. Alternatively, this work explores the feasibility of blood pulsation imaging under incoherent green illumination for measuring the response of human body on local thermal impact. The proposed technique allows assessment of the cutaneous blood flow changes during thermoregulation simultaneously in different areas of the body. The preliminary experiments show that the blood pulsation amplitude (BPA) is sufficiently reliable index, which could characterize the relative change of the cutaneous blood flow similarly to the parameter measured with the Laser Doppler Flowmetry technique. It is shown that BPA grows up proportionally to the skin temperature increase in the preliminary cooled finger, whilst it is in the steady state in another finger having the constant temperature. The rate of BPA increase is individual characteristic of a subject, which could serve as a parameter of the subject's vasomotor reactivity on the temperature changes. High quality of visualized distribution of blood pulsations, good repeatability of the BPA, and revealed dependencies of its response on the skin-temperature change offer the prospect for development new system of studying microcirculation.

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Non-Invasive Measurement of Skin Microvascular Response during Pharmacological and Physiological Provocations

Cited by 57 publications

References 44 publications

Skin glucose metabolism and microvascular blood flow during local insulin delivery and after an oral glucose load

Skin glucose metabolism and microvascular blood flow during local insulin delivery and after an oral glucose load

Perfusion of the skin’s microcirculation after cold‐water immersion (10°C) and partial‐body cryotherapy (−135°C)

Local Thermal Impact on Microcirculation Assessed by Imaging Photoplethysmography

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