Non-Invasive Microelectrode Ion Flux Measurements In Plant Stress Physiology

Role of proline under changing environments

“…2). 89 Reports indicate that proline is responsible for scavenging the ROS and other free radicals. 35,36,[90][91][92][93][94] Proline, when applied exogenously to roots of Arabidopsis, resulted in a reduced level of ROS, indicating the ROS scavenging potential of proline.…”

Pathways of cadmium fluxes in the root of the halophyte Suaeda salsa

“…Due to methodological limitations (relatively poor spatial and temporal resolution), these experiments failed to provide answers about the specific ionic uptake mechanisms involved. given us a new opportunity to successfully address the issues raised above (Newman, 2001;Newman et al, 1987;Shabala, 2003Shabala, , 2006Kunkel et al, 2001Kunkel et al, , 2005Xu et al, 2006). The technique now allows us to measure specific ion fluxes from different regions of the root under practical physiological conditions (Newman, 2001;Pineros et al, 1998).…”

Ion flux profiles and plant ion homeostasis control under salt stress

“…17 It is suggested that NaCl stress induces a stimulation of H + -ATPases, which provides a driving force for PM Na + /H + antiporters to move Na + from the cytoplasm into the apoplast, thereby making an important contribution to the maintenance of low Na + in the cytosol. 11 Using the MIFE, a significant increase in net H + efflux were recorded from the NaCl-shocked mesophyll tissues of a broad bean species (Vacia faba, salt-sensitive). 13 The increased H + efflux was inhibited by orthovanadate (a specific inhibitor of the PM H + pump), indicating that PM H + -ATPase is involved in the cellular responses to salt stress.…”

“…In recent years, the non-invasive measurement of ion fluxes is becoming an important approach to elucidate the dynamic changing of ion relations that caused by salinity. 11,12 Up to the present, there are two typical but similar systems that designed to measure ion fluxes across membranes, i.e., the SIET (Scanning Ion-selective Electrode Technique) and the MIFE (Microelectrode Ion Flux Estimation) technique. 11,12 These systems are proposed to measure electrochemical potential differences between two positions close to the tissues or cells by moving the ion-selective microelectrodes in corresponding measuring solutions.…”

“…11,12 Up to the present, there are two typical but similar systems that designed to measure ion fluxes across membranes, i.e., the SIET (Scanning Ion-selective Electrode Technique) and the MIFE (Microelectrode Ion Flux Estimation) technique. 11,12 These systems are proposed to measure electrochemical potential differences between two positions close to the tissues or cells by moving the ion-selective microelectrodes in corresponding measuring solutions. Then the net fluxes of target elements are calculated according to the ion gradients which are converted from the measured electrochemical potentials (reviewed in refs.…”