Non-invasive prediction of blastocyst implantation, ongoing pregnancy and live
            birth, by mass spectrometry lipid fingerprinting

Borges, Edson; Braga, D.P.A.F.; Setti, Amanda Souza; Montanni, Daniela A.; Cabral, Elaine C.; Eberlin, Marcos N.; Turco, E.G. Lo; Iaconelli, Assumpto

doi:10.5935/1518-0557.20160044

Cited by 3 publications

(1 citation statement)

References 38 publications

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“…Lipids are another class of molecules that might have some prediction power in blastulation and pregnancy (Borges et al, 2016), however, the results in this field are still preliminary.…”

Section: Non-invasive Tools: Beyong the Pgtmentioning

confidence: 99%

The embryo non-invasive pre-implantation diagnosis era: how far are we?

del Collado,

Andrade,

Gonçalves

et al. 2023

Anim. Reprod.

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Advancements in assisted reproduction (AR) methodologies have allowed significant improvements in live birth rates of women who otherwise would not be able to conceive. One of the tools that allowed this improvement is the possibility of embryo selection based on genetic status, performed via preimplantation genetic testing (PGT). Even though the widespread use of PGT from TE biopsy helped to decrease the interval from the beginning of the AR intervention to pregnancy, especially in older patients, in AR, there are still many concerns about the application of this invasive methodology in all cycles. Therefore, recently, researchers started to study the use of cell free DNA (cfDNA) released by the blastocyst in its culture medium to perform PGT, in a method called non-invasive PGT (niPGT). The development of a niPGT would bring the diagnostics power of conventional PGT, but with the advantage of being potentially less harmful to the embryo. Its implementation in clinical practice, however, is under heavy discussion since there are many unknowns about the technique, such as the origin of the cfDNA or if this genetic material is a true representative of the actual ploidy status of the embryo. Available data indicates that there is high correspondence between results observed in TE biopsies and the ones observed from cfDNA, but these results are still contradictory and highly debatable. In the present review, the advantages and disadvantages of niPGT are presented and discussed in relation to tradition TE biopsy-based PGT. Furthermore, there are also presented some other possible non-invasive tools that could be applied in the selection of the best embryo, such as quantification of other molecules as quality biomarkers, or the use artificial intelligence (AI) to identify the best embryos based on morphological and/or morphokitetic parameters.

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“…Lipids are another class of molecules that might have some prediction power in blastulation and pregnancy (Borges et al, 2016), however, the results in this field are still preliminary.…”

Section: Non-invasive Tools: Beyong the Pgtmentioning

confidence: 99%

The embryo non-invasive pre-implantation diagnosis era: how far are we?

del Collado,

Andrade,

Gonçalves

et al. 2023

Anim. Reprod.

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The long noncoding RNA PTENP1 regulates human endometrial epithelial adhesive capacity in vitro: implications in infertility

Takamura

Zhou

Rombauts

et al. 2019

Biology of Reproduction

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There is general consensus that the synchronous development of the embryo and endometrium is absolutely essential for successful implantation. Recent studies have strongly suggested that embryo-secreted factors are able to deliver into the endometrial cavity/endometrium and alter its protein profile in preparation for implantation. However, there is limited research focusing on long noncoding RNA (lncRNA) changes in the endometrium that brought about by the embryonic derived factors. It has been suggested that lncRNA has intricate interplay with microRNA (miR), small (~19–22 nucleotides), non-protein-coding RNA, to regulate protein production in the endometrium, thus controlling adhesive capacity. Here through microarray assays, we compare the lncRNA profile of the primary human endometrial epithelial cells (HEECs) that have been precultured with blastocyst-conditioned media (BCM) from embryos that implanted versus nonimplanted. Our data indicate a substantial change of lncRNA expression in HEECs, including 9 up-regulated and 12 down-regulated lncRNAs after incubation with implanted BCM. Selective knockdown of PTENP1, the most increased lncRNA after implanted BCM treatment in the HEECs, compromised the spheroid adhesion (P < 0.001). Characterization of PTENP1 confirmed its expression in the luminal epithelium with staining appeared most intense in the midsecretory phase. Furthermore, we have recorded a substantial change of miR profile upon PTENP1 knockdown in HEECs. Overexpression of miR-590-3p, a novel predicted target of PTENP1, impaired spheroid adhesion (P < 0.001). Collectively, these data have supported a novel regulation system that lncRNAs were able to participate in the regulation of implantation through association with miRs.

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OMICS technologies in reproductive medicine: assessment of quality of oocytes and embryos

2018

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One of the main factors of success of the procedure art (assisted reproductive technology) is the selection of the most "high-quality” gametes for further manipulation and obtaining a viable embryo for implantation. The majority of modern techniques based on morphokinetic predictors of quality (i. e. assessment of embryo morphology and rate of division of the blastomeres), which allowed to achieve some success in increasing the percentage of successful pregnancies and reduce the number of multiple pregnancies, but their accuracy is currently insufficient. Thus, the development of objective, reliable, fast and affordable test systems to determine the quality of oocytes and the development potential of the embryo - one of the challenges of reproductive medicine. The purpose of this review was to describe the advantages and limitations obecnych technologies, the application of which will allow to deepen our understanding of the physiology of the embryo, as well as set criteria for non-invasive selection of gametes and embryos. In this regard, recently in assisted reproduction are applied the studies of genomic, proteomic, transcript, and metabolomic profiles of oocytes, granulosa and Cumulus cells, embryos, of conditioned media.

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Non-invasive prediction of blastocyst implantation, ongoing pregnancy and live birth, by mass spectrometry lipid fingerprinting

Cited by 3 publications

References 38 publications

The embryo non-invasive pre-implantation diagnosis era: how far are we?

The embryo non-invasive pre-implantation diagnosis era: how far are we?

The long noncoding RNA PTENP1 regulates human endometrial epithelial adhesive capacity in vitro: implications in infertility

OMICS technologies in reproductive medicine: assessment of quality of oocytes and embryos

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